Co-delivery of antibiotic and baicalein by using different polymeric nanoparticle cargos with enhanced synergistic antibacterial activity

Guo, Qianqian; Guo, Honglei; Lan, Tianyu; Chen, Yi; Chen, Xueyun; Feng, Yakai; Luo, Yongjun; Yao, Yifang; Li, Yafei; Pan, Xiuhao; Xu, Yini; Tao, Ling; Liu, Yujia; Shen, Xiangchun

doi:10.1016/j.ijpharm.2021.120419

Cited by 10 publications

(6 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The charge of the complex remains positive and makes it easier to internalize into cells based on the interaction with the negatively charged cell membranes ( Lo C.-W. et al, 2015 ; Demirer et al, 2019 ). Poly (2-( N,N -dimethylamino) ethyl methacrylate) (PDMAEMA) and polyethylenimine (PEI) are two typical cationic polymers with a high density of positively charged amine groups, and have both been used for DNA delivery in animal cells ( Wu et al, 2011 ; Yu et al, 2012 ; Lo C.-W. et al, 2015 ; Guo et al, 2021 ; Richter et al, 2021 ). Yet, their applications in plant cells are rarely investigated.…”

Section: Introductionmentioning

confidence: 99%

Efficient Transient Expression of Plasmid DNA Using Poly (2-(N,N-Dimethylamino) Ethyl Methacrylate) in Plant Cells

Cao

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Nanomaterials have been widely studied for their potential to become the new generation of nanocarriers in gene transfection, yet it remains still difficult to apply them efficiently and succinctly to plant cells. Poly (2-(N,N-dimethylamino) ethyl methacrylate) (PDMAEMA), which possesses temperature and pH dual-sensitivity, has largely been applied in animal cells, but it is rarely involved in plant cells. As a proof of concept, PDMAEMA as a gene carrier is incubated with plasmid GFP (pGFP) to explore its transfection ability in plants, and cationic polymer polyethylenimine (PEI) is used as a control. pGFP was efficiently condensed into the nanostructure by electrostatic interactions at an N/P (amino group from cationic polymers/phosphate group from plasmid DNA (pDNA)) ratio of 15; after complexation into nanocarriers, pGFP was protected from endonuclease degradation according to the DNase I digestion assay. After incubation with protoplasts and leaves, GFP was observed with confocal microscopy in plant cells. Western blot experiments confirmed GFP expression at the protein level. Toxicity assay showed PDMAEMA had a lower toxicity than PEI. These results showed that transient expression of pGFP was readily achieved in Arabidopsis thaliana and Nicotiana benthamiana. Notably, PDMAEMA showed lower cytotoxicity than PEI upon incubation with Nicotiana benthamiana leaves. PDMAEMA exhibited great potency for DNA delivery in plant cells. This work provides us with new ideas of more concise and more effective methods for plant transformation.

show abstract

Section: Introductionmentioning

confidence: 99%

Efficient Transient Expression of Plasmid DNA Using Poly (2-(N,N-Dimethylamino) Ethyl Methacrylate) in Plant Cells

Cao

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…BE has been included in several herbal preparations to treat widespread health conditions (Rossi et al, 2001). Currently, BE is being considered for its antiviral, antibacterial, anti‐inflammatory, anti‐tumor, and anti‐allergic effects (Guo et al, 2021; Phan et al, 2020; Rui et al, 2020; Susmitha et al, 2020; Xu et al, 2019; Zandi et al, 2012).…”

Section: Baicaleinmentioning

confidence: 99%

Baicalein: A review on its anti‐cancer effects and mechanisms in lung carcinoma

Naveenkumar¹,

Anandakumar

2022

Journal of Food Biochemistry

View full text Add to dashboard Cite

Plant-derived flavonoids are reported to function as potential anti-cancer agents against different types of cancer. Baicalein (BE) is an important flavonoid found in the roots of Scutellaria baicalensis that is popularly used in Chinese medicine as an ingredient in herbal tea preparations to promote wellness. BE has been studied for its several biological effects including antioxidant, anti-inflammatory, anti-hepatotoxic, antiviral, and anti-tumor properties. BE has now been discovered to be an effective agent against lung neoplasm. The molecular factors supporting baicalein's anti-cancer activity against lung cancer and its value to human health are discussed in this article. This would help in identifying BE as a promising competent drug against lung carcinoma. Practical applicationsBaicalein is a flavonoid obtained from the roots of Scutellaria baicalensis. It has been widely used as an antioxidant, anti-inflam5matory, anti-hepatotoxic, antiviral, and anti-cancer agent. Lung cancer is one of the most common malignancies in the world with a high fatality rate. Several studies have found that Baicalein is an important candidate for treating lung cancer. Its mechanism of action includes regulation of cell proliferation, metastasis, apoptosis, autophagy, and so on. Baicalein could be used as a novel anti-cancer drug for the treatment of lung carcinoma.

show abstract

“…An immense number of antibacterial drugs have been developed, and the subsequent emergence of antimicrobial-resistant bacteria has since become a serious global issue. , With this in mind, novel antibacterial materials have attracted significant attention including low-molecular-weight peptides, , metal nanoparticles and nanocomposites, , and cationic polymers. − Antibacterial (or antimicrobial) materials are categorized as either release-killing agents or contact-killing agents. , More than 2000 research articles on antibacterial polymer nanoparticles have been published; typically, the polymers are conjugated with inorganic nanoparticles, such as Ag, Au, ZnO, Cu, and Fe. In addition, polymer nanoparticles are also used as a cargo for antibacterial drugs. , Therefore, a synergy effect can be achieved for antibacterial nanoparticles by utilizing cationic polymers. However, the antibacterial activity of polymers themselves has not been discussed sufficiently.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, polymer nanoparticles are also used as a cargo for antibacterial drugs. 13,14 Therefore, a synergy effect can be achieved for antibacterial nanoparticles by utilizing cationic polymers. However, the antibacterial activity of polymers themselves has not been discussed sufficiently.…”

Section: ■ Introductionmentioning

confidence: 99%

Surface Characteristics of Antibacterial Polystyrene Nanoparticles Synthesized Using Cationic Initiator and Comonomers

Suga

Murakami

Nakayama

et al. 2022

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Polymer nanoparticles have attracted attention as antibacterial materials, but the function of the polymer itself has not yet been clarified sufficiently. To estimate the essential surface properties of antibacterial polymer nanoparticles, herein, we synthesized cationic polystyrene (PSt) nanoparticles via soap-free emulsion polymerization using 2,2′-azobis-[2-(1,3-dimethyl-4,5-dihydro-1H-imidazol-3-ium-2-yl)]propane triflate (ADIP) as initiator. The conversion of total monomers was drastically increased through the addition of the commoner (vinylbenzyl)trimethylammonium chloride (VBTMAC), where unimodal size distributions (C v ≤ 10%) were obtained at comonomer molar ratios between 0.0083 and 0.0323. The adsorption behavior of a solvatochromic anionic fluorescent dye revealed the surface charge density (σ) and affinity with anionic molecules (K) of PSt nanoparticles. The PSt nanoparticles with increased K values exhibited antibacterial activity against Staphylococcus epidermidis, with a minimum inhibitory concentration of at least 0.69 mg/mL. To determine a plausible mechanism for the antibacterial activity, the membrane damage induced by PSt nanoparticles was evaluated using an assay utilizing polydiacetylene vesicles as the model for negatively charged bilayer membranes. The PSt nanoparticles exhibiting large K values disturbed the bilayer structure of the model membrane system, suggesting that the synthesized PSt nanoparticles could be utilized as a contact-killing antibacterial agent.

show abstract

Co-delivery of antibiotic and baicalein by using different polymeric nanoparticle cargos with enhanced synergistic antibacterial activity

Cited by 10 publications

References 33 publications

Efficient Transient Expression of Plasmid DNA Using Poly (2-(N,N-Dimethylamino) Ethyl Methacrylate) in Plant Cells

Efficient Transient Expression of Plasmid DNA Using Poly (2-(N,N-Dimethylamino) Ethyl Methacrylate) in Plant Cells

Baicalein: A review on its anti‐cancer effects and mechanisms in lung carcinoma

Surface Characteristics of Antibacterial Polystyrene Nanoparticles Synthesized Using Cationic Initiator and Comonomers

Contact Info

Product

Resources

About