Co-development of naive CD4+ cells towards T helper Type 1 or T helper type 2 cells induced by a combination of IL.-12 and IL-4

Palm, Norbert; Germann, Tieno; Goedert, Sigrid; Hoehn, Petra; Roelsch, Stephan; Rüde, Erwin; Schmitt, Edgar

doi:10.1016/s0171-2985(97)80064-2

Cited by 13 publications

(6 citation statements)

References 22 publications

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“…The work of Emery et al (1996) have shown that a sequential and combined Th1/ Th2 cytokine profile is associated with prolonged metacestode growth and survival, as well as with partial protection of the host. This mixed Th1 (IL-12) and Th2 (IL-10) response may be due to the simultaneous development of Th1 and Th2 cells, which are not necessarily mutually exclusive (Palm et al 1996). Consequently, the results taken together indicate that the antigens of Echinococcus, in the in vitro model, generated a response which was associated with a mixed Th1/ Th2 response.…”

Section: Discussionmentioning

confidence: 85%

An in vitro model to evaluate the cytokine response in Echinococcus infections

Fraize

Sarciron

Saboulard

et al. 2004

Parasitology Research

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With the aim of proposing an alternative model to animal experimentation, we investigated cytokine production in response to antigens in an in vitro system. This is a co-culture system of healthy human leukocytes and enterocyte-like Caco-2 cells. The antigens tested, EgA31, EgTrp, and FABP1, are candidates for vaccines in infections caused by Echinococcus spp. in the gut. All three have previously been described in the protoscolex stage and belong to protein families which confer protective immunity against several helminths. In this study, we evaluate the Th1/Th2 profile (Th1: IL-12, IFN-gamma; Th2: IL-6, IL-10) in response to protoscoleces, EgA31 and the mixture of EgA31, EgTrp and FABP1. No cytokine production was detected in response to protoscoleces. Neither IFN-gamma nor IL-6, but a significant IL-10 and IL-12 concentration was detected in response to both types of antigens. These findings suggest that EgA31 and the mixture EgA31/EgTrp/FABP1 generated an immunogenic response associated with a mixed Th1/Th2 cytokine.

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Section: Discussionmentioning

confidence: 85%

An in vitro model to evaluate the cytokine response in Echinococcus infections

Fraize

Sarciron

Saboulard

et al. 2004

Parasitology Research

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“…The Th1/Th2 balance plays a crucial role in the maintenance of immune homeostasis. IL-12 is a potent differentiation factor for Th1 cells, whereas IL-4 promotes the development of Th2 cells (22). Some studies have reported that LAB exert antiallergic activity by modulating the Th1/Th2 balance toward a Th1-predominant condition and suppressing the serum total IgE level when administered orally to BALB/c mice (23,24).…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Lactic Acid Bacteria Isolated from Traditional South Asian Fermented Milk `Dahi' Inhibits the Development of Atopic Dermatitis in NC/Nga Mice

Watanabe

Hamada

Tategaki

et al. 2009

J Nutr Sci Vitaminol

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SummaryWe investigated the suppressive effect of lactic acid bacteria (LAB) isolated from traditional South Asian fermented milk 'dahi' on the development of atopic dermatitis (AD) using NC/Nga AD model mice. In the initial evaluation, we confirmed the effect of LAB on serum total IgE using ovalbumin (OVA)-induced type 1 allergy model mice. Forty-one bacterial strains isolated from dahi were evaluated for their ability to induce interleukin (IL)-12 production and suppress IL-4 production in splenocytes obtained from OVA-sensitized mice. Of the 41 strains tested, Lactobacillus delbrueckii subsp. lactis R-037 exhibited the greatest IL-12 induction, suggesting that it is a potent Th1 inducer. Oral administration of heattreated R-037 significantly suppressed the elevation of serum total IgE in OVA-induced type 1 allergy model mice. In NC/Nga AD model mice, oral administration of heat-treated R-037 reduced inflammatory auricular thickness and alleviated the AD clinical score although the effect on serum total IgE level was unclear. Histopathological findings showed a tendency toward improvement of inflammation. Hyperkeratosis in particular showed improvement in dermatitis skin lesions. These results suggest that oral administration of R-037 may alleviate AD.

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“…Segregation of T cells to Th1͞Th2 is based only on the level of cytokine secretion because intracellular determination of cytokines show that the dichotomy is not necessarily absolute (2)(3)(4).…”

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confidence: 99%