Co-digestion of wheat and rye bread suspensions with source-sorted municipal biowaste

Li, Chaoran; Mörtelmaier, Christoph; Winter, Josef; Gallert, Claudia

doi:10.1016/j.wasman.2015.03.024

Cited by 6 publications

(5 citation statements)

References 33 publications

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“…In the present study Methanoculleus sp. were not specially enumerated, but a positive correlation between Pelotomaculum and the number of Methanomicrobiales (Pearson correlation 0.79, [ 6 ]) was seen, which was in accordance with data from earlier work with the same source of biowaste [ 7 , 26 ]. There are, however, also examples of both high numbers of Pelotomaculum sp.…”

Section: Discussionsupporting

confidence: 88%

“…Co-digestion of propionate seems to be a way to stabilize and increase the number of POB. Compared with the POB numbers in investigations with biowaste alone, the numbers of POB in digesters that were fed with biowaste + propionate were slightly higher: After the start of the full-scale biowaste digester of Karlsruhe 2–3 × 10 8 POB per mL were found in reactor effluent [ 6 ] and during co-digestion of biowaste with wheat and rye bread at high concentrations of propionate 2–5 × 10 8 POB per mL were detected [ 26 ], whereas 5–6 × 10 8 POB per mL were found after biowaste/propionate co-digestion ( Figure 6 c).…”

Section: Discussionmentioning

confidence: 99%

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Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate

Moertelmaier

Winter

et al. 2015

Bioengineering

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Propionate is the most delicate intermediate during anaerobic digestion as its degradation is thermodynamically unfavorable. To determine its maximum possible degradation rates during anaerobic digestion, a reactor was fed Monday to Friday with an organic loading rate (OLR) of 12/14 kg CODbiowaste·m−3·d−1 plus propionate up to a final OLR of 18 kg COD·m−3·d−1. No feed was supplied on weekends as it was the case in full-scale. To maintain permanently high propionate oxidizing activity (POA), a basic OLR of 3 kg CODpropionate·m−3·d−1 all week + 11 kg CODbiowaste·m−3·d−1 from Monday to Friday was supplied. Finally a reactor was operated with an OLR of 12 kg CODbiowaste·m−3·d−1 from Monday to Friday and 5 kg CODpropionate·m−3·d−1 from Friday night to Monday morning to maintain a constant gas production for permanent operation of a gas engine. The propionate degradation rates (PDRs) were determined for biowaste + propionate feeding. Decreasing PDRs during starvation were analyzed. The POA was higher after propionate supply than after biowaste feeding and decreased faster during starvation of a propionate-fed rather than a biowaste-fed inoculum. Shifts of the propionate-oxidizing and methanogenic community were determined.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate

Moertelmaier

Winter

et al. 2015

Bioengineering

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“…Culture-independent molecular analyses of methanogenic communities based on next-generation sequencing have revealed that the contribution of methanogens performing the acetoclastic, methanotrophic, or hydrogenotrophic pathways in anaerobic digesters depends on the nature of the substrate and the process conditions [ 42 , 46 – 49 ]. We previously discussed this issue and presented arguments in favor of the dominance of the hydrogenotrophic pathway of methane generation in bioreactors processing the acidic effluent from molasses fermentation [ 3 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle

Detman

Mielecki

Pleśniak

et al. 2018

Biotechnol Biofuels

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BackgroundAnaerobic digestion, whose final products are methane and carbon dioxide, ensures energy flow and circulation of matter in ecosystems. This naturally occurring process is used for the production of renewable energy from biomass. Lactate, a common product of acidic fermentation, is a key intermediate in anaerobic digestion of biomass in the environment and biogas plants. Effective utilization of lactate has been observed in many experimental approaches used to study anaerobic digestion. Interestingly, anaerobic lactate oxidation and lactate oxidizers as a physiological group in methane-yielding microbial communities have not received enough attention in the context of the acetogenic step of anaerobic digestion. This study focuses on metabolic transformation of lactate during the acetogenic and methanogenic steps of anaerobic digestion in methane-yielding bioreactors.ResultsMethane-yielding microbial communities instead of pure cultures of acetate producers were used to process artificial lactate-rich media to methane and carbon dioxide in up-flow anaerobic sludge blanket reactors. The media imitated the mixture of acidic products found in anaerobic environments/digesters where lactate fermentation dominates in acidogenesis. Effective utilization of lactate and biogas production was observed. 16S rRNA profiling was used to examine the selected methane-yielding communities. Among Archaea present in the bioreactors, the order Methanosarcinales predominated. The acetoclastic pathway of methane formation was further confirmed by analysis of the stable carbon isotope composition of methane and carbon dioxide. The domain Bacteria was represented by Bacteroidetes, Firmicutes, Proteobacteria, Synergistetes, Actinobacteria, Spirochaetes, Tenericutes, Caldithrix, Verrucomicrobia, Thermotogae, Chloroflexi, Nitrospirae, and Cyanobacteria. Available genome sequences of species and/or genera identified in the microbial communities were searched for genes encoding the lactate-oxidizing metabolic machinery homologous to those of Acetobacterium woodii and Desulfovibrio vulgaris. Furthermore, genes for enzymes of the reductive acetyl-CoA pathway were present in the microbial communities.ConclusionsThe results indicate that lactate is oxidized mainly to acetate during the acetogenic step of AD and this comprises the acetotrophic pathway of methanogenesis. The genes for lactate utilization under anaerobic conditions are widespread in the domain Bacteria. Lactate oxidation to the substrates for methanogens is the most energetically attractive process in comparison to butyrate, propionate, or ethanol oxidation.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1106-z) contains supplementary material, which is available to authorized users.

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“…According to the results of the study described in research [3], the re lease of biogas at a fermentation temperature of 38°с from substrates with the addition of rye bread is 0.459 l/g dry organic matter (DoM), in research [4], various types of grain release -from 0.634 l/g DoM (for rye bread) to 0.943 l/g DoM (for wheat bread with egg and milk additives). As it is stated in research [5], the common fermentation of biowaste with the waste of wheat and rye bread with an organic loading ra te of 14 kg/m 3 per day, was stable to their organic loading rate kg/m 3 per day and enabled producing 3 times mo re biogas. The release of methane at the fermentation of grain with DoM in the proportion of 1:9, 3:7 and 5:5 is 233 ml/g volatile substance (Vs), 298 ml/g Vs and 344 ml/g Vs [6].…”

mentioning

confidence: 86%

“…The largest release of methane was obtained from the substrate with the addition of grain inclusions (2546 ml), whereas with the addition of fruit was te, 2000 ml was obtained, vegetable waste -1468 ml. in research [13,5], the release of biogas from barley grains is estimated as 353-658 m 3 /t Vs, from wheat as 384-426 m 3 /t Vs, from triticale as 337-555 m 3 /t Vs, from oats as 250-295 m 3 /t Vs, from sorghum as 295-372 m 3 /t Vs, and from rye as 283-492 m 3 /t Vs. The release of methane from processed brewing grain, as stated in researches [14] and [15], is 0.284 l/g of hsC.…”

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confidence: 99%

Increasing the Bio Gas Release During the Cattle Manure Fermentation by Means the Rational Addition of Substandard Flour as a Cosubstrate

Поліщук¹,

Romaniuk²,

Tarasenko³

et al. 2020

Nauka innov.

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The processing of livestock waste and agricultural production enables not only improving environmental safety, but also obtaining energy resources. Problem Statement. Cow manure is used to produce biogas, but its yield is relatively low. One way to increase biogas yield is the joint fermentation of cow manure with substandard flour, which cannot be used as human food and as feed for farm animals. However, the flour content in the substrate, at which the biogas yield is maximum and there is no inhibition of the methane fermentation process, is not a solved issue. Purpose. Increase biogas yield by adding substandard flour to a cow manure-based substrate. Materials and Methods. Research of biogas yield during methane fermentation of substrate based on cow manure with the addition of substandard flour was carried out on a biogas plant consisting of a methane tank with a working volume of 30 l and wet gasholder at periodic substrate loading and fermentation temperature 35 °C. The portion of the substrate included 1.7 kg of manure, 2.5 kg of water and 50, 100, 250 or 500 g of flour. The composition of flour in the substrate was 1.2%, 2.3%, 5.6% and 10.6%. The dichotomy method was used to determine the optimal flour content in the substrate, provided the maximum biogas yield. Results. The maximum biomethane release is 14.3 l/kg dry organic matter (DOM), at 1.

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Co-digestion of wheat and rye bread suspensions with source-sorted municipal biowaste

Cited by 6 publications

References 33 publications

Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate

Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate

Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle

Increasing the Bio Gas Release During the Cattle Manure Fermentation by Means the Rational Addition of Substandard Flour as a Cosubstrate

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