Co-existence of plasmid-mediated quinolone resistance determinants and mutations in gyrA and parC among fluoroquinolone-resistant clinical Enterobacteriaceae isolated in a tertiary hospital in Warsaw, Poland

Piekarska, Katarzyna; Wołkowicz, Tomasz; Zacharczuk, Katarzyna; Rzeczkowska, Magdalena; Chróst, Anna; Bareja, Elzbieta; Olak, Monika; Gierczyński, Rafał

doi:10.1016/j.ijantimicag.2014.09.019

Cited by 64 publications

(78 citation statements)

References 23 publications

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“…In Enterobacteriaceae, qnrS, qnrB and aac(6 )-Ib-cr genes have been implicated in plasmid-mediated quinolone resistance (PMQR) [57,58]. A combination of low-level resistance to fluoroquinolones caused by PMQR mechanism, with QRDR mutation can lead to clinically relevant resistance [59]. We did not find any PMQR in the fluoroquinolone resistant isolates exhibiting QRDR, indicating that these mutations were only identified in resistant isolates.…”

Section: Concordance Of Amr Phenotype and Genotypementioning

confidence: 74%

Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials

Stanford¹,

Zaheer

Klima

et al. 2020

Microorganisms

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Over a two-year period, Mannheimia haemolytica (MH; n = 113), Pasteurella multocida (PM; n = 47), Histophilus somni (HS; n = 41) and Mycoplasma bovis (MB; n = 227) were isolated from bovine lung tissue at necropsy from cattle raised conventionally (CON, n = 29 feedlots) or without antimicrobials [natural (NAT), n = 2 feedlots]. Excluding MB, isolates were assayed by PCR to detect the presence of 13 antimicrobial resistance (AMR) genes and five core genes associated with integrative and conjugative elements (ICEs). Antimicrobial susceptibility phenotypes and minimum inhibitory concentrations (MICs, µg/mL) were determined for a subset of isolates (MH, n = 104; PM, n = 45; HS, n = 23; and MB, n = 61) using Sensititre analyses. A subset of isolates (n = 21) was also evaluated by whole-genome sequencing (WGS) based on variation in AMR phenotype. All five ICE core genes were detected in PM and HS by PCR, but only 3/5 were present in MH. Presence of mco and tnpA ICE core genes in MH was associated with higher MICs (p < 0.05) for all tetracyclines, and 2/3 of all macrolides, aminoglycosides and fluoroquinolones evaluated. In contrast, association of ICE core genes with MICs was largely restricted to macrolides for PM and to individual tetracyclines and macrolides for HS. For MH, the average number of AMR genes markedly increased (p < 0.05) in year 2 of the study due to the emergence of a strain that was PCR positive for all 13 PCR-tested AMR genes as well as two additional AMR genes (aadA31 and blaROB-1) detected by WGS. Conventional management of cattle increased (p < 0.05) MICs of tilmicosin and tulathromycin for MH; neomycin and spectinomycin for PM; and gamithromycin and tulathromycin for MB. The average number of PCR-detected AMR genes in PM was also increased (p < 0.05) in CON mortalities. This study demonstrates increased AMR especially to macrolides by bovine respiratory disease organisms in CON as compared to NAT feedlots and a rapid increase in AMR following dissemination of strain(s) carrying ICE-associated multidrug resistance.

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Section: Concordance Of Amr Phenotype and Genotypementioning

confidence: 74%

Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials

Stanford¹,

Zaheer

Klima

et al. 2020

Microorganisms

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“…Analysis of quinolone resistance-determining regions of gyrA and parC genes revealed the presence of multiple and diverse mutations in gyrA (S83Y, S83F, D87A)and parC (S80I, N304S) in isolates that were clonally distinct. Mutations in gyrA and parC genes have been reported as major mechanisms of fluoroquinolone/quinolone resistance associated with DNA gyrase and topoisomerase IV alterations in Enterobacteriaceae (Alvi et al, 2018;Piekarska et al, 2015; Sekyere and Amoako, 2017), as the plasmid-mediated quinolone resistance genes (PMQR) and extrusion by intrinsic efflux pumps commonly mediate low-level fluoroquinolone/quinolone resistance (Sekyere and Amoako, 2017). However, mutations in both gyrA and parC are often common and associated with high-level quinolone resistance in Enterobacteriaceae compared with alterations in gyrB (Piekarska et al, 2015) as evident in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Mutations in gyrA and parC genes have been reported as major mechanisms of fluoroquinolone/quinolone resistance associated with DNA gyrase and topoisomerase IV alterations in Enterobacteriaceae (Alvi et al, 2018;Piekarska et al, 2015; Sekyere and Amoako, 2017), as the plasmid-mediated quinolone resistance genes (PMQR) and extrusion by intrinsic efflux pumps commonly mediate low-level fluoroquinolone/quinolone resistance (Sekyere and Amoako, 2017). However, mutations in both gyrA and parC are often common and associated with high-level quinolone resistance in Enterobacteriaceae compared with alterations in gyrB (Piekarska et al, 2015) as evident in this study. ST101 (P26-75, P26-81) and ST152 (P26-66) isolates were of the same mutation codons 83 and 87 of the gyrA and at 80 in parC genes with no mutation in gyrB gene among isolates with a ciprofloxacin MIC of 4 mg/L.…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of multidrug-resistant ESBL-producing Klebsiella pneumoniae isolated from a Ghanaian teaching hospital

Agyepong

Govinden

Owusu-Ofori

et al. 2019

International Journal of Infectious Diseases

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This study delineated the clonal lineages, antibiotic resistome and plasmid replicon types in multidrug-resistant K. pneumoniae isolates from a teaching hospital in Ghana. Methods: Identification and antibiotic susceptibility testing were done using the MALDI-TOF MS and Vitek-2 automated system. Genomic DNA extraction was carried out using the NucliSens easyMAG1 (BioMérieux) kits and the DNA was subjected to whole genome sequencing (WGS) using the Illumina MiSeq platform. Results: Of the 200 isolates obtained, 37 were identified as K. pneumoniae of which 9 were resistant to all second and third-generation cephalosporins. These 9 isolates selected for further genomic analysis were characterized by the presence of 8 diverse sequence types (STs), capsular polysaccharide serotypes (K types and wzi allelic types) and multiple genes encoding resistance to β-lactams (bla CTX-M-15 , bla SHV-11, bla TEM-1B, bla OXA-1), aminoglycosides (aac(3)-IIa, strB, strA, aadA16), fluoroquinolones/quinolones (qnrB66, oqxA, oqxB) and other antibiotic classes. Resistance genes were associated with plasmids, predominantly IncFIB(K) and ColRNAI. Multiple and diverse mutations in quinolone resistance-determining regions of gyrA (S83Y, D87A) and parC (S80I, N304S) in isolates resistant to ciprofloxacin (MIC 4 mg/mL) were found. Global phylogenomic analysis affirmed the diverse clonal clustering and origin of these isolates. Conclusions: The varied clonal clusters and resistome identified in the multidrug-resistant K. pneumoniae isolates is a major threat to the management of infections in Ghana. The molecular characterization of antibiotic resistance is thus imperative to inform strategies for containment.

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“…Efflux pump QepA was the most common isolated genes. According to Piekarska K et al 2015, PMQR determinants were present in 49 urinary isolates (22.8%), among which aac(6')-Ib-cr and proteins Qnr predominated (85.7% and 26.5%, respectively) [15]. But Marchisio M et al did not find any plasmid-mediated quinolone resistance determinants among Enterobacteriaceae isolated from urinary tract infections patients [23].…”

Section: Discussionmentioning

confidence: 96%

PMQR Determinants Expression in Gram-negative Uropathogens Clinically Isolated from Hospitalized Patients with Pyelonephritis in Kharkiv, Ukraine

Чуб¹,

Bilchenko²,

Teslenko³

2017

TOUNJ

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Co-existence of plasmid-mediated quinolone resistance determinants and mutations in gyrA and parC among fluoroquinolone-resistant clinical Enterobacteriaceae isolated in a tertiary hospital in Warsaw, Poland

Cited by 64 publications

References 23 publications

Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials

Antimicrobial Resistance in Members of the Bacterial Bovine Respiratory Disease Complex Isolated from Lung Tissue of Cattle Mortalities Managed with or without the Use of Antimicrobials

Genomic characterization of multidrug-resistant ESBL-producing Klebsiella pneumoniae isolated from a Ghanaian teaching hospital

PMQR Determinants Expression in Gram-negative Uropathogens Clinically Isolated from Hospitalized Patients with Pyelonephritis in Kharkiv, Ukraine

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