Co-exposure to aluminum and acrylamide disturbs expression of metallothionein, proinflammatory cytokines and induces genotoxicity: Biochemical and histopathological changes in the kidney of adult rats

Ghorbel, Imen; Maktouf, Sameh; Fendri, N.; Jamoussi, Kamel; Châabouni, Semia Ellouz; Boudawara, Tahia; Zeghal, Najiba

doi:10.1002/tox.22114

Cited by 19 publications

(15 citation statements)

References 55 publications

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“…After this glomerular filtration, between 40% and 60% is reabsorbed at the tubular level, constituting a marker of renal function [ [17] , [18] , [19] ]. In the present study, blood urea was elevated in acrylamide-treated rats (50 mg/kg), suggesting kidney damage [ [20] , [21] , [22] , [23] ]. Another metabolite that was analyzed in the present study was creatinine.…”

Section: Discussionmentioning

confidence: 64%

“…Its elimination is exclusively renal, so there is a correlation between creatinine levels and renal function. Most creatinine that is eliminated by the kidneys is freely filtered in renal glomeruli, and a small fraction is filtered by the tubular component, which is a good indicator of renal-glomerular function [ 17 , [21] , [22] , [23] ]. Similar to urea, creatinine and BUN significantly increased in rats that received acrylamide (25 and 50 mg/kg), indicating imminent kidney damage.…”

Section: Discussionmentioning

confidence: 99%

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Alterations of blood chemistry, hepatic and renal function, and blood cytometry in acrylamide-treated rats

Rivadeneyra-Domínguez

Becerra-Contreras

Vázquez-Luna

et al. 2018

Toxicology Reports

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Acrylamide is a vinyl monomer that is widely used for the synthesis of polyacrylamides, the treatment of drinking water, and as an additive in cosmetics. Acrylamide is also produced during the thermal processing of carbohydrate-rich foods. Although the potential toxic effects of acrylamide have been reported, few studies have evaluated biochemical parameters in blood. The present study investigated alterations of blood chemistry, hepatic function, and blood cytometry in acrylamide-treated rats. Thirty-two male Wistar rats were assigned to four experimental groups (n = 8/group): one control group received 0.3 ml of vehicle (saline solution), and the other three groups received acrylamide (25, 50, and 75 mg/kg, i.p., for 14 days). At the end of treatment, blood samples were collected to obtain serum, which was then processed using a Vitros250 device. For blood cytometry, the samples were processed in a Sysmex analyzer. The blood chemistry results showed that urea nitrogen, urea, and creatinine were elevated in the acrylamide-treated groups. Tests of hepatic function showed that total and direct bilirubins, transaminases, and alkaline phosphatase were also elevated compared with vehicle, whereas the levels of total proteins and albumin decreased. Blood cytometry showed that the levels of erythrocytes, hemoglobin, hematocrit, leukocytes, and platelets and mean cell volume decreased in the acrylamide-treated groups compared with vehicle. Overall, the present findings indicate that acrylamide causes deleterious effects on renal and hepatic physiology, producing dose-dependent alterations of blood chemistry and cytometry parameters in male Wistar rats.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Alterations of blood chemistry, hepatic and renal function, and blood cytometry in acrylamide-treated rats

Rivadeneyra-Domínguez

Becerra-Contreras

Vázquez-Luna

et al. 2018

Toxicology Reports

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“…Acrylamide and aluminum (Al) are recognized worldwide as serious environmental contaminants. Coexposure to these compounds caused toxicity in soft tissues like liver (Ghorbel et al 2015a), kidney (Ghorbel et al 2015b), and heart (Ghorbel et al 2015c) of adult rats. The most usual Al exposure for the general population is through the diet, mainly from dietary additives (Fekete et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Dietary unsaponifiable fraction of extra virgin olive oil supplementation attenuates lung injury and DNA damage of rats co-exposed to aluminum and acrylamide

Ghorbel

Chaâbane

Boudawara

et al. 2016

Environ Sci Pollut Res

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Aluminum chloride (AlCl3) and acrylamide (ACR) are well known as environmental pollutants inducing oxidative stress. Our study investigated the effects of these contaminants and if the hydrophilic fraction of extra virgin olive oil was able to prevent lung oxidative stress and DNA damage. Animals were divided into four groups of six each: group 1, serving as controls, received distilled water; group 2 received in drinking water aluminum chloride (50 mg/ kg body weight) and by gavage acrylamide (20 mg/kg body weight); group 3 received both aluminum and acrylamide in the same way and the same dose as group 2 and hydrophilic fraction from olive oil (OOHF) (1 ml) by gavage; group 4 received only OOHF by gavage. Exposure of rats to both aluminum and acrylamide provoked oxidative stress in lung tissue based on biochemical parameters and histopathological alterations. In fact, we have observed an increase in malondialdehyde (MDA), H2O2, and advanced oxidation protein product (AOPP) and a decrease in reduced glutathione (GSH), non-protein thiols (NPSH), and vitamin C levels. Activities of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also decreased. Histopathological changes in lung tissue were noted like emphysema, vascular congestion, and infiltration of inflammatory cells. A random DNA degradation was observed on agarose gel in the lung of AlCl3 and acrylamide (ACR)-treated rats. Co-administration of OOHF to treated rats improved biochemical parameters to near control values and lung histoarchitecture. The smear formation of genomic DNA was reduced. The hydrophilic fraction of extra virgin olive oil might provide a basis for developing a new dietary supplementation strategy in order to prevent lung tissue damage.

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“…The cells lining renal tubules showed cytoplasmic vacuolations and loss of brush borders. 35,36 Our results from ACR-treated group revealed that the renal corpuscle appeared with significant increase in the amount of collagen fibers deposition among its glomerular capillaries. This is can explain by, ACR induced oxidative stress, resulting in generation of reactive oxygen species ROS which plays a critical role in the initiation and progression of fibrotic diseases.…”

Section: Discussionmentioning

confidence: 65%

Effect of vitamin E on biochemical and ultrastructural changes in acrylamide-induced renal toxicity in rats

et al. 2017

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Background: Acrylamide (ACR) is a widely used chemical in industry and it accounts for major health problems as it has been detected in highly consumed food items, carbohydrate-rich food items cooked at high temperature. Accordingly the population is highly exposed to ACR. The aim of the study was to assess the effect of vitamin E on biochemical and ultrastructure changes in acrylamide-induced renal toxicity in wistar albino rats.Methods: Three groups of adult albino wistar rats weighing about (200-250 gm) were used in this study to investigate the effect of vitamin E on acrylamide induced renal toxicity; 10 rats in each group; Group I: Control group, Group II: Acrylamide treated and Group III: Acrylamide-Vit E treated group. Blood samples were collected for estimation of serum creatinine, blood urea nitrogen (BUN), lactic dehydrogenase (LDH) and albumin. Kidneys specimens were processed for light and electron microscopic studies. Kidney sections were stained with H&E, MT, PAS and immuno-histochemical stains for detection of NF-kβp65 and Bcl-2. Morphometric study was done followed by biochemical and morphometric statistical analysiss.Results: Acrylamide treated rats showed degeneration of cells lining PCT and DCT, atrophy of glomeruli and fibrosis. Ultrastructurally; tubules lining cells showed loss of microvilli, basal membrane in foldings and mitochondrial changes. Podocytic changes include: Vacuolation, irregularity and disorientation of their processes and thinning of glomerular basement membrane. Significant increase in the mean number of NF-kβp65 positive cells and decrease in the mean area% of Bcl-2 immuno-expression, increase in the mean area % of collagen fibers and urinary space diameter and decrease in thickness of epithelial cells of PCT and DCT were also observed. Serum creatinine, BUN anLDH were increased whereas serum albumin was decreased. Vitamin E co-administration with ACR improve all these histological, ultrastructural and biochemical changes.Conclusions: Acrylamide induced renal toxicity could be ameliorated by vitamin E co-administration.

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Co-exposure to aluminum and acrylamide disturbs expression of metallothionein, proinflammatory cytokines and induces genotoxicity: Biochemical and histopathological changes in the kidney of adult rats

Cited by 19 publications

References 55 publications

Alterations of blood chemistry, hepatic and renal function, and blood cytometry in acrylamide-treated rats

Alterations of blood chemistry, hepatic and renal function, and blood cytometry in acrylamide-treated rats

Dietary unsaponifiable fraction of extra virgin olive oil supplementation attenuates lung injury and DNA damage of rats co-exposed to aluminum and acrylamide

Effect of vitamin E on biochemical and ultrastructural changes in acrylamide-induced renal toxicity in rats

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