Co-expression of Parathyroid Hormone and Chromogranin Ain Secondary Hyperparathyroidism: A Functional Marker for Secretory Activity of Hyperplastic Nodules

Tanaka, Reika; Umemura, Shinobu; Kakuta, Tsunekazu; Fujisaki, Tomotaka; Sakai, Hideto; Saitoh, Akira; Osamura, R. Yoshiyuki

doi:10.1078/0344-0338-00360

Cited by 7 publications

(10 citation statements)

References 14 publications

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“…Interestingly, no considerable amount of PTH was detected in the cytosol of TMSC differentiated after longer periods of differentiation (21 days). Furthermore, we found increased expression of CHGA protein, a parathyroid secretory granulespecific marker [19,20], which was clearly colocalized with PTH protein in differentiated TMSC (Fig. 1B).…”

Section: Pth Protein Is Expressed and Colocalizes With Chga In Differmentioning

confidence: 77%

Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

et al. 2015

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Section: Pth Protein Is Expressed and Colocalizes With Chga In Differmentioning

confidence: 77%

Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

et al. 2015

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“…Staining differences of PTH and chromogranin A in normal, hyperplastic, and neoplastic parathyroid lesions have been documented. 5,18,20 Parathyroid neoplasms were more common than expected in this population of nondomestic felids, when compared with domestic cats, but an IHC panel may be required for accurate diagnosis. Most of the thyroid neoplasms and all of the parathyroid neoplasms in these nondomestic felids were benign.…”

Section: Discussionmentioning

confidence: 85%

Spontaneous proliferative and neoplastic lesions in thyroid and parathyroid glands of nondomestic felids

et al. 2016

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Based on microscopic and immunohistochemical characterization, we documented spontaneous proliferative and neoplastic lesions in the thyroid and parathyroid glands of nondomestic felids. Ten animals (4 leopards, 3 tigers, and 3 cougars), all with a previous diagnosis of thyroid neoplasia were identified from the University of Tennessee College of Veterinary Medicine database. The mean age of affected animals was 15.9 y. Twelve neoplasms were identified; 2 animals had 2 concurrent neoplasms. After immunohistochemical characterization using a panel of chromogranin A, thyroglobulin, and calcitonin, 7 of the former thyroid neoplasms were diagnosed as thyroid adenomas, 1 was diagnosed as a thyroid carcinoma, and 4 were diagnosed as parathyroid adenomas. No thyroid medullary neoplasms (C-cell tumors) were diagnosed in the current study. Most of the diagnosed neoplasms were benign (11 of 12), and metastasis was not documented in the single carcinoma. Only 2 animals were suspected to have functional neoplasms (1 thyroid adenoma and 1 parathyroid adenoma), based on associated tissue lesions or serum biochemistry. Other documented lesions in the thyroid and parathyroid glands included thyroid nodular hyperplasia ( n = 7), parathyroid hyperplasia associated with chronic renal disease ( n = 2), a thyroid abscess, and a branchial cyst. Parathyroid adenomas were more commonly diagnosed than expected in comparison with domestic cats. We demonstrated that an immunohistochemistry panel for thyroglobulin, calcitonin, and chromogranin A can be used to differentiate neoplasms of thyroid from parathyroid origin in nondomestic felids.

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“…PTH is accumulated in secretory vesicles containing CHGA and it is secreted in response to signals. CHGA can be a good marker for the regular processes of PTH production and secretion [20,21]. We could confirm the normal synthesis of PTH and related secretory granule, CHGA from the implanted parathyroid tissue on immunohistochemical staining (Fig.…”

Section: Discussionmentioning

confidence: 86%

Development of hypoparathyroidism animal model and the feasibility of small intestinal submucosa application on the parathyroid autotransplantation

Park¹,

Jung²,

Kim³

et al. 2014

Eur Arch Otorhinolaryngol

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The purpose of this study is to evaluate the feasibility of small intestinal submucosa (SIS) application on the parathyroid autotransplantation in a rat model of hypoparathyroidism. The rats were divided into four groups: NC (no procedure, n = 5), PTX (total parathyroidectomy, n = 6), PT (total parathyroidectomy and parathyroid autotransplantation, n = 10) and PT + SIS group (total parathyroidectomy and parathyroid autotransplantation with SIS, n = 10). The levels of parathyroid hormone (PTH), calcium, and phosphorous were measured on 0, 3, 7, 21, 56 and 84 days after surgery. PTH level was expressed as median (interquartile range) and histological and immunohistochemical examinations were performed. PTH levels were significantly decreased to "not detectable level" from day 3 in PTX group. PTH was not detected in both PT and PT + SIS groups on the 21st day. On the 56th day, PTH levels were increased in both groups: 3 out of 8 rats (37.5%) in the PT group, 6 out of 9 rats (66.7%) in the PT + SIS group. The PTH level was fully recovered to its preoperative range on the day 84 as 6 of 8 rats (75%) of the PT group and 7 of 9 rats (77.8%) of the PT + SIS group were recovered; the PTH levels were 117.84 and 178.36 pg/ml, respectively. The neo-vascularization was well observed around the parathyroid tissue, and the number of new vessels formed was higher in the PT + SIS group (15 vessels/high power field) as compared to the PT group (10 vessels/high power field). This study showed the feasibility and the treatment effect of SIS as the success rate of autotransplantation of parathyroid tissue was significantly increased without severe inflammatory response in hypothyroidism animal model.

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Co-expression of Parathyroid Hormone and Chromogranin Ain Secondary Hyperparathyroidism: A Functional Marker for Secretory Activity of Hyperplastic Nodules

Cited by 7 publications

References 14 publications

Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

Differentiated tonsil-derived mesenchymal stem cells embedded in Matrigel restore parathyroid cell functions in rats with parathyroidectomy

Spontaneous proliferative and neoplastic lesions in thyroid and parathyroid glands of nondomestic felids

Development of hypoparathyroidism animal model and the feasibility of small intestinal submucosa application on the parathyroid autotransplantation

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