Co-immobilized enzymes in magnetic chitosan beads for improved hydrolysis of macromolecular substrates under a time-varying magnetic field

Yang, Kun; Xu, Ning-Shou; Su, Wei

doi:10.1016/j.jbiotec.2010.05.001

Cited by 53 publications

(28 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to the studies on single enzyme immobilization with nanomaterials, the investigations on multi-enzyme immobilization are considerably less. Nevertheless, many industrial bioconversions often involve more than one enzyme, such as in situ cofactor regeneration system, thus studies on multienzyme immobilization are important for understanding these systems (Ei-Zahab et al 2008;Yang et al 2010). In addition to circumventing common problems associated with single enzyme immobilization-enzyme leaching (Kim et al 2006a), strong diffusion resistance (Yi et al 2006), and loss of enzyme 3D structure (Klibanov 1983), other factors such as ratios between enzymes and overall optimal reaction conditions need to be examined in multi-enzymatic immobilization.…”

Section: Introductionmentioning

confidence: 99%

Nanotube-supported bioproduction of 4-hydroxy-2-butanone via in situ cofactor regeneration

Wang

Zhang

Ching

et al. 2011

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Nicotinamide cofactor-dependent oxidoreductases have been widely employed during the bioproduction of varieties of useful compounds. Efficient cofactor regeneration is often required for these biotransformation reactions. Herein, we report the synthesis of an important pharmaceutical intermediate 4-hydroxy-2-butanone (4H2B) via an immobilized in situ cofactor regeneration system composed of NAD(+)-dependent glycerol dehydrogenase (GlyDH) and NAD(+)-regenerating NADH oxidase (nox). Both enzymes were immobilized on functionalized single-walled carbon nanotubes (SWCNTs) through the specific interaction between the His-tagged enzymes and the modified SWCNTs. GlyDH demonstrated ca. 100% native enzyme activity after immobilization. The GlyDH/nox ratio, pH, and amount of nicotinamide cofactor were examined to establish the optimum reaction conditions for 4H2B production. The nanoparticle-supported cofactor regeneration system become more stable and the yield of 4H2B turned out to be almost twice (37%) that of the free enzyme system after a 12-h reaction. Thus, we believe that this non-covalent specific immobilization procedure can be applied to cofactor regeneration system for bioconversions.

show abstract

Section: Introductionmentioning

confidence: 99%

Nanotube-supported bioproduction of 4-hydroxy-2-butanone via in situ cofactor regeneration

Wang

Zhang

Ching

et al. 2011

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Chitosan due to its unique physico-chemical and biological properties is an attractive material for use in various applications (Sorlier et al, 2001;Yang et al, 2010). These properties include: biodegradability, lack of toxicity, antibacterial and antifungal effects, wound healing acceleration and immune system stimulation (Shi et al, 2011a).…”

Section: Applications Of Chitosanmentioning

confidence: 99%

“…3b) make it potentially useful in immobilization of various enzymes (Fang et al, 2009). Results of several studies have been shown that after selection of suitable enzyme immobilization method, chitosan reactive amino and hydroxyl groups offer a good enzyme coupling efficiency (Luo et al, 2005;Biro et al, 2009;Yang et al, 2010).…”

Section: Immobolized Enzymes Onto Chitosanmentioning

confidence: 99%

“…Chitosan is known as an ideal support material for enzyme immobilization because of its many characteristics like improved resistance to chemical degradation and avoiding disturbance of metal ions to enzyme (Vazquez-Duhalt et al, 2001;Yang et al, 2010). Enzymes have been immobilized onto chitosan supports with different particle size.…”

Section: Chitosan Nanoparticles As Enzyme Immobilization Supportmentioning

confidence: 99%

“…When the pH is higher than pK a , the amino groups are deprotonated and chitosan becomes insoluble. Because of its excellent film forming ability, biocompatibility, nontoxicity, high mechanical strength, cheapness and a susceptibility to chemical modifications, chitosan has been extensively used for immobilization of enzymes (Vaillant et al, 2000;Kilinc et al, 2002;Wei et al, 2002;Krajewska, 2004;Liang et al, 2005;Luo et al, 2005;Tang et al, 2006;Yang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Malmiri

Jahanian

Berenjian

2012

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Chitosan is an attractive natural biopolymer from renewable resources with the presence of reactive amino and hydroxyl functional groups in its structure. Due to the good biocompatibility of chitosan, it can be used in magnetic-field assisted drug delivery, enzyme or cell immobilization and many other industrial applications. In the past decade, nanotechnology has been a considerable research interest in the area of preparation of immobilized enzyme carriers. This study looks at characteristics and applications of chitosan and chitosan nanoparticles and their potentials as suitable supports for enzyme immobilization. Results indicated that activity of immobilized enzymes and performance of enzyme immobilization onto chitosan nanoparticles are higher than chitosan macro and microparticles. As compared to other biopolymers nanoparticles, application of chitosan nanoparticles to immobilize enzymes strongly increases stability of immobilized enzymes and their easy separability from the reaction mixture at the end of the biochemical process.

show abstract

Preparation of amino‐reserved magnetic chitosan microsphere and its application in adsorbing endotoxin

Lai

Jiang

et al. 2012

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this article, we present a novel amino‐reserved magnetic chitosan microsphere (ARMCM) prepared from chitosan and Fe3O4 through inverse suspension crosslinking by formaldehyde and epichlorohydrin. The density of ARMCM was 1.47 g/mL, and the amino content was 2.44 mmol/g. The measurements data indicated that the saturation magnetization (σs) of ARMCM was 23.2 emu/g. The adsorption results showed that these magnetic chitosan microspheres had good adsorption capacity on endotoxin: the maximum absorption capacity was about 1792.1EU/g in 40 min; the endotoxin adsorption efficiency in the protein solution was close to 80% and what is more, protein adsorption efficiency was only 23.2 and 2.0%, respectively, for acidic and basic proteins. It is expected that this ARMCM will have a feasible and useful application in adsorbing endotoxin from biological products. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012

show abstract

Co-immobilized enzymes in magnetic chitosan beads for improved hydrolysis of macromolecular substrates under a time-varying magnetic field

Cited by 53 publications

References 18 publications

Nanotube-supported bioproduction of 4-hydroxy-2-butanone via in situ cofactor regeneration

Nanotube-supported bioproduction of 4-hydroxy-2-butanone via in situ cofactor regeneration

Potential Applications of Chitosan Nanoparticles as Novel Support in Enzyme Immobilization

Preparation of amino‐reserved magnetic chitosan microsphere and its application in adsorbing endotoxin

Contact Info

Product

Resources

About