Co-Induction of a Glutathione-S-transferase, a Glutathione Transporter and an ABC Transporter in Maize by Xenobiotics

Pang, Sen; Liu, Duan; Liu, Zhiqian; Song, Xiaoyu; Li, Xuefeng; Wang, Chengju

doi:10.1371/journal.pone.0040712

Cited by 40 publications

(35 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These changes are similar to those reported for untreated A. myosuroides plants, including the detection of specific phi GSTs in MHR but not HS plants [13]. However, none of the immunoreactive GST proteins in HS or MHR A. fatua were induced to higher levels after treatment with three different herbicide mechanisms of action, in direct contrast to results from A. myosuroides [69], maize [70], and other species [71]. The A. fatua results are supported by a lack of transcriptional induction of four GSTs in response to flucarbazone-sodium treatment of HS plants (Keith et al in press).…”

Section: Discussionsupporting

confidence: 84%

Proteomic and biochemical assays of glutathione-related proteins in susceptible and multiple herbicide resistant Avena fatua L.

Burns

Keith

Refai

et al. 2017

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

Extensive herbicide usage has led to the evolution of resistant weed populations that cause substantial crop yield losses and increase production costs. The multiple herbicide resistant (MHR) Avena fatua L. populations utilized in this study are resistant to members of all selective herbicide families, across five modes of action, available for A. fatua control in U.S. small grain production, and thus pose significant agronomic and economic threats. Resistance to ALS and ACCase inhibitors is not conferred by target site mutations, indicating that non-target site resistance mechanisms are involved. To investigate the potential involvement of glutathione-related enzymes in the MHR phenotype, we used a combination of proteomic, biochemical, and immunological approaches to compare their constitutive activities in herbicide susceptible (HS1 and HS2) and MHR (MHR3 and MHR4) A. fatua plants. Proteomic analysis identified three tau and one phi glutathione S-transferases (GSTs) present at higher levels in MHR compared to HS plants, while immunoassays revealed elevated levels of lambda, phi, and tau GSTs. GST specific activity towards 1-chloro-2,4-dinitrobenzene was 1.2-fold higher in MHR4 than in HS1 plants and 1.3- and 1.2-fold higher in MHR3 than in HS1 and HS2 plants, respectively. However, GST specific activities towards fenoxaprop-P-ethyl and imazamethabenz-methyl were not different between untreated MHR and HS plants. Dehydroascorbate reductase specific activity was 1.4-fold higher in MHR than HS plants. Pretreatment with the GST inhibitor NBD-Cl did not affect MHR sensitivity to fenoxaprop-P-ethyl application, while the herbicide safener and GST inducer mefenpyr reduced the efficacy of low doses of fenoxaprop-P-ethyl on MHR4 but not MHR3 plants. Mefenpyr treatment also partially reduced the efficacy of thiencarbazone-methyl or mesosulfuron-methyl on MHR3 or MHR4 plants, respectively. Overall, the GSTs described here are not directly involved in enhanced rates of fenoxaprop-P-ethyl or imazamethabenz-methyl metabolism in MHR A. fatua. Instead, we propose that the constitutively elevated GST proteins and related enzymes in MHR plants are representative of a larger, more global suite of abiotic stress-related changes.

show abstract

Section: Discussionsupporting

confidence: 84%

Proteomic and biochemical assays of glutathione-related proteins in susceptible and multiple herbicide resistant Avena fatua L.

Burns

Keith

Refai

et al. 2017

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

show abstract

“…5,6 Safeners (formerly called antidotes, antagonists, or protectants) are chemicals that enhance the ability of crops (but not weeds) to detoxify herbicides. 4,7 Safeners can, for example, promote in vivo transformations of herbicides into products with less or no appreciable herbicidal activity (for reviews on modes of action, see refs 3, 5, and 8−12). Safeners are typically applied as either seed treatments (e.g., with sorghum), water applications (rice), or spray formulations (corn and other cereals) containing one or more herbicides and adjuvants.…”

Section: ■ Introductionmentioning

confidence: 99%

Environmental Fate and Effects of Dichloroacetamide Herbicide Safeners: “Inert” yet Biologically Active Agrochemical Ingredients

Sivey

Lehmler

Salice

et al. 2015

Environ. Sci. Technol. Lett.

View full text Add to dashboard Cite

Safeners are included in many commercial herbicide formulations to selectively protect crops from injury induced by active ingredients. Despite their bioactivity, safeners are classified as inert from a regulatory perspective, and as such, safeners have received minimal attention in the peer-reviewed literature regarding their environmental fate and effects. Herein, we review what is known about the uses, physicochemical properties, environmental transformations, and (eco)toxicological effects of dichloroacetamide safeners, which represent one of the most commonly used safener classes (estimated use of >2 × 10 6 kg/year in the United States). We particularly highlight transformation pathways that may enhance biological activity and/or persistence; for example, limited studies suggest dichloroacetamides can transform via dechlorination into products with increased bioactivity. We also identify several research needs to improve our understanding of the environmental fate and potential risks of this overlooked agrochemical class, which in turn will enhance the efficacy and safety of future herbicide safener formulations.

show abstract

“…In addition to transformation, toxins may be conjugated to glucose, malonyl, or glutathione by transferases, transforming them into more water-soluble compounds [30, 69, 71–75] that are then translocated into the vacuole or cell wall compartments [69, 76]. Interestingly in Arabidopsis , the expression of 12 glutathione transferases increase under phenanthrene exposure, including At4g02520 , At2g02930 , At1g02920 , and At1g02930 [34, 77].…”

Section: Discussionmentioning

confidence: 99%

Detoxification of polycyclic aromatic hydrocarbons (PAHs) in Arabidopsis thaliana involves a putative flavonol synthase

Hernández-Vega

Cady

Kayanja

et al. 2017

Journal of Hazardous Materials

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons (PAHs) are environmental contaminants with cytotoxic, teratogenic and carcinogenic properties. Bioremediation studies with bacteria have led to the identification of dioxygenases (DOXs) in the first step to degrade these recalcitrant compounds. In this study, we characterized the role of the Arabidopsis thaliana AT5G05600, a putative DOX of the flavonol synthase family, in the transformation of PAHs. Phenotypic analysis of loss-of-function mutant lines showed that these plant lines were less sensitive to the toxic effects of phenanthrene, suggesting possible roles of this gene in PAH degradation in vivo. Interestingly, these mutant lines showed less accumulation of H2O2 after PAH exposure. Transgenic lines over-expressing At5g05600 showed a hypersensitive response and more oxidative stress after phenanthrene treatments. Moreover, fluorescence spectra results of biochemical assays with the recombinant His-tagged protein AT5G05600 detected chemical modifications of phenanthrene. Taken together, these results support the hypothesis that AT5G05600 is involved in the catabolism of PAHs and the accumulation of toxic intermediates during PAH biotransformation in plants. This research represents the first step in the design of transgenic plants with the potential to degrade PAHs, leading to the development of vigorous plant varieties that can reduce the levels of these pollutants in the environment.

show abstract

Co-Induction of a Glutathione-S-transferase, a Glutathione Transporter and an ABC Transporter in Maize by Xenobiotics

Cited by 40 publications

References 30 publications

Proteomic and biochemical assays of glutathione-related proteins in susceptible and multiple herbicide resistant Avena fatua L.

Proteomic and biochemical assays of glutathione-related proteins in susceptible and multiple herbicide resistant Avena fatua L.

Environmental Fate and Effects of Dichloroacetamide Herbicide Safeners: “Inert” yet Biologically Active Agrochemical Ingredients

Detoxification of polycyclic aromatic hydrocarbons (PAHs) in Arabidopsis thaliana involves a putative flavonol synthase

Contact Info

Product

Resources

About