Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle

Kumar, Pawan; Jangir, Babu Lal; Saikumar, G.; Somvanshi, R.

doi:10.17221/7178-vetmed

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…Teats revealed grossly flat-and-round type, frond-like, and rice grain-like growths, usually multiple in numbers on all teat skin. Similar to our findings, previous researchers (8,22) reported teat warts with similar morphological appearances associated with flower-like and frond-type growths caused by BPV-1 and -10. Squamous papillomas of teats were usually characterized by epidermal thickening, acanthosis, numerous koilocytes, and hyperkeratosis.…”

Section: Discussionsupporting

confidence: 93%

“…Teat papillomatosis is very common in cows, and it regresses spontaneously in the field. Outbreaks can reach serious proportions and teats often become secondarily infected, and sometimes the invasion of tumors can be so severe that milking becomes impossible (3,5,8,22). Teats revealed grossly flat-and-round type, frond-like, and rice grain-like growths, usually multiple in numbers on all teat skin.…”

Section: Discussionmentioning

confidence: 99%

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Pathological and immunohistochemical evaluation of skin and teat papillomas in cattle

Beytut¹

2017

Turk J Vet Anim Sci

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Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3 + T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3 + T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. αSMA positivity was mainly in the media of vessels. Smooth muscle cells of the dermal blood vessels showed an immunopositive reaction for desmin. Dermal connective tissue and squamous epithelia were negative for S-100 protein. This work revealed that BPV proliferates predominantly in the stratum granulosum cells and that in the regression of warts cell-mediated immune response has a key role. Vimentin was a very useful marker to show the contribution of fibrotic response to viral replication.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Pathological and immunohistochemical evaluation of skin and teat papillomas in cattle

Beytut¹

2017

Turk J Vet Anim Sci

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“…It has been established that both BPV-1 and -2 are the etiological agents of cutaneous and teat warts in cattle (12,13). BP is a known disease in cattle, while in buffaloes few sporadic cases have been reported in the Indian subcontinent and it is an unrecognized entity (2).…”

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confidence: 99%

Pathological studies and detection of different bovine papilloma virus types in buffalo cutaneous warts

Jangir¹,

Bind²,

Kumar³

et al. 2017

Turk J Vet Anim Sci

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Bovine papillomatosis (BP) is distributed worldwide among cattle but is relatively less common in buffaloes. Prevalence of this disease is established in buffaloes from India (1,2) and Italy (3) caused by bovine papilloma virus (BPV). BPV is a nonenveloped double stranded DNA virus with icosahedral symmetry that infects the cutaneous and mucosal epithelia inducing hyperplastic lesions. Viral capsid proteins production takes place only in productive infections. The virion particles assemble in the granular and cornified layers; mature virus particles are released on disintegration of dead squamous cells (4). So far, 13 different BPV types have been identified and characterized. These are classified as Deltapapillomavirus (BPV-1, -2, and -13), Epsilonpapillomavirus (BPV-5 and -8), and Xipapillomavirus , with the exception of BPV-7, which belongs to an as yet unassigned papillomavirus genus (5-8). Papillomatosis in buffaloes was reviewed and it was opined that it is an almost unknown disease in buffaloes and is caused by BPV-1 and -2 or their mixed infections. Once infection of BPV establishes in buffaloes, it spread from buffalo to buffalo, with no intermediate involvement of cattle (2). Various markers are expressed during cell division, each with a specific function. Expression of Ki-67 nuclear antigen in all phases of the cell-cycle except for G 0 has been recently found to be the most reliable indicator of cellular proliferation (9). Keeping the above in mind, the present work was carried out with the objective to study the occurrence and association of different BPV types with cutaneous warts (CWs) in Indian buffaloes, and their pathomorphological assessment.CWs/tumor biopsies from buffaloes were collected from the Referral Veterinary Polyclinic IVRI, Izatnagar, Government Veterinary Hospitals, different villages around Bareilly, India, and two archived samples from West Bengal, India. Biopsies were collected after administration of local anesthesia around the growth, samples were preserved in 10% buffered formalin for histopathological studies, and a part of samples showing gross lesions were also stored in sterile vials at -20 °C for molecular studies. Detailed gross observations with specific locations of growths/ wart-like lesions were recorded. After proper fixation, the representative tissues were dehydrated in increasing grades of alcohol, cleared in xylene, and embedded in paraffin blocks. Sections with 4-5 µm thickness were cut and stained with hematoxylin and eosin (H&E) as per the conventional procedure.DNA was extracted from the tissue samples using the Genomic DNA Mini Kit as per the manufacturer's Abstract: Bovine papillomatosis is a common viral disease of cattle but is relatively less common in buffaloes. Bovine papilloma virus (BPV) -1 and -2 are mostly associated with cutaneous warts in buffaloes. The aim of the present work was to study the pathomorphology and association of different BPV types in buffalo cutaneous warts. A total of 10 cases were included in the present study, which reveale...

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“…Warts present on the udder also interfere with the milking process (Radostitis et al, 2007). This disease affects animals older than 2 years; however, bovine animals of all ages can develop these lesions (Kumar et al, 2013). Immunosuppressive factors play a role in the progression of bovine papillomatosis, as mentioned by Radostitis et al (2007), including internal and external parasites.…”

Section: Introductionmentioning

confidence: 99%

First report of bovine papillomavirus genotypes in cutaneous lesions by polymerase chain reaction (PCR) and direct sequencing in Panama

GonzÃ¡lez¹,

Murillo²,

Castillo³

et al. 2019

Afr. J. Biotechnol.

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Bovine papillomavirus (BPV) causes benign tumours in the mucosal and cutaneous epithelium and is characterized by the presence of warts. The present study includes the molecular identification of BPV strains in samples of warts using degenerate polymerase chain reaction (PCR) primers FAP59/64. Skin biopsies were taken from crossbred cattle from two experimental farms of Instituto de Investigación Agropecuaria de Panamá (IDIAP) during the period from July 2016 to February 2018. Fourteen samples were positive by PCR amplification and sequenced at the laboratory of the Institute of Legal Medicine and Forensic Sciences (IMELCF). The analysis of the sequences allowed the identification of strains related to seven viral types; this was the first time that this type of study was carried out in Panama. The present study showed that PCR amplification with the primers FAP59/64, which partially amplify the L1 gene, followed by direct sequencing was useful for genotyping BPV. This study possibly identified local strains of BPV2 and BBA2; however, it is necessary to carry out more studies to establish the diversity and distribution of this virus in the country. The results in this study are important for the development of prophylactic and therapeutic measures that contribute to reducing the economic losses associated with BPV in Panama.

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Co-infection of bovine papillomavirus type-1 and -10 in teat warts of dairy cattle

Cited by 5 publications

References 19 publications

Pathological and immunohistochemical evaluation of skin and teat papillomas in cattle

Pathological and immunohistochemical evaluation of skin and teat papillomas in cattle

Pathological studies and detection of different bovine papilloma virus types in buffalo cutaneous warts

First report of bovine papillomavirus genotypes in cutaneous lesions by polymerase chain reaction (PCR) and direct sequencing in Panama

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