Co-infection of two criniviruses and a begomovirus enhances the disease severity in cucumber

Abrahamian, Peter; Sobh, H.; Seblani, Rewa; Abou‐Jawdah, Y.

doi:10.1007/s10658-015-0630-y

Cited by 24 publications

(10 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, whiteflies transmit more than 114 species of plant viruses, many of which cause economically important plant diseases such as tomato yellow leaf curl virus (TYLCV), cotton leaf curl virus (CLVC), cucumber vein yellowing virus (CVYV), etc. [ 1 , 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Sub-Lethal Effects of Partially Purified Protein Extracted from Beauveria bassiana (Balsamo) and Its Presumptive Role in Tomato (Lycopersicon esculentum L.) Defense against Whitefly (Bemisia tabaci Genn.)

Keerio

Nazir

Anwar

et al. 2020

Insects

View full text Add to dashboard Cite

Plants rely on various physiological and molecular defense mechanisms against biotic stresses such as herbivore insects. Many entomopathogenic fungi synthesize protein molecules that can trigger these plant defenses. This laboratory study characterized the bioactivity of a partially purified protein derived from Beauveria bassiana (ARSEF 2860) against whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), which is an economically important pest of agricultural and horticultural crops worldwide. Different concentrations (i.e., 0.021, 0.042 and 0.063 μM) of fungal protein were bioassayed to determine their sub-lethal effect on the survival percentage and fecundity rate of B. tabaci on tomato (Lycopersicon esculentum) plants. In addition, the putative role of this partially purified B. bassiana protein in the defense mechanisms of plant was assessed through the expression analyses of important genes related to salicylic acid (SA)—and jasmonic acid (JA)—associated pathways using RT-qPCR. Results revealed a significant suppression of the survival percentage and fecundity rate of B. tabaci by the fungal protein. Lowest survival (41%) was recorded for the highest concentration of protein (0.063 μM), whereas mean survival for the other two protein concentrations (0.042 and 0.021 μM) were 62 and 71%, respectively. Likewise, the highest and lowest mean fecundity rates were observed for the control and the highest protein concentration (i.e., 3.3 and 1.8 eggs day−1 female−1, respectively). Furthermore, the exogenous application of B. bassiana-derived protein on tomato plants strongly up-regulated the SA-related genes (PAL, PR1, BGL2 and EDS1) and slightly up-regulated the JA-related genes (AOC, AOS, OPR3 and LOX) as compared to the control plants. These findings demonstrate the putative role of this partially purified B. bassiana protein fraction in inducing systemic resistance in the tomato plants against B. tabaci, suggesting its further purification and characterization to be used as novel biological pest control tool against B. tabaci and other sap-sucking insect pests.

show abstract

Section: Introductionmentioning

confidence: 99%

Sub-Lethal Effects of Partially Purified Protein Extracted from Beauveria bassiana (Balsamo) and Its Presumptive Role in Tomato (Lycopersicon esculentum L.) Defense against Whitefly (Bemisia tabaci Genn.)

Keerio

Nazir

Anwar

et al. 2020

Insects

View full text Add to dashboard Cite

show abstract

“…Criniviruses are phloem‐limited and known to cause symptoms of interveinal leaf yellowing, leaf brittleness and reduction of plant vigour. Although no direct damage is observed on fruit, total yield reductions may vary from 0.2% to 63% (Abrahamian et al ., ; Mansilla‐Córdova et al ., ), mostly due to the loss of photosynthetic activity. Symptoms on infected plants are easily mistaken for nutritional or physiological disorders, including magnesium and/or manganese deficiencies, as well as pesticide toxicities (Wisler & Duffus, ).…”

Section: Introductionmentioning

confidence: 99%

Criniviruses associated with cucurbit yellows disease in Greece and Cyprus: an ever‐changing scene

et al. 2019

View full text Add to dashboard Cite

Cucurbit yellow stunting disorder virus (CYSDV), Cucurbit chlorotic yellows virus (CCYV) and Beet pseudo-yellows virus (BPYV) are whitefly-transmitted criniviruses that cause foliar interveinal yellowing symptoms and result in high economic losses for cucurbit production. CYSDV and CCYV are transmitted by Bemisia tabaci, whereas BPYV is transmitted by Trialeurodes vaporariorum. During 2012-2017, an extensive survey was conducted to identify the viruses causing cucurbit yellows disease in Greece and Cyprus. The study sampled the main cucurbits and alternative hosts in these regions to determine crinivirus incidence, to identify the whitefly species present in the two countries and to characterize molecularly the virus populations. Results showed that CYSDV was the most widespread virus in Greece (49.9%), followed by CCYV (20.3%) and BPYV (18.4%). Bemisia tabaci and T. vaporariorum were identified in 54.5% and 45.6% of whitefly samples, respectively. In Cyprus, CYSDV was predominant (96.7%), followed by CCYV (19.2%), while no BPYV infection was detected. Approximately 15% of weed samples from 17 different species that belong to 12 botanical families were identified as hosts for one or more of these criniviruses. Finally, sequencing of the capsid protein gene of the crinivirus isolates revealed very low levels of genetic diversity, further supporting the genetic stability of crinivirus populations. The results of this long-lasting epidemiological study in two countries of the eastern Mediterranean revealed substantial changes in the relative incidence and distribution of cucurbit-infecting criniviruses and their whitefly vectors over the past 15 years, suggesting the need for adoption of novel management strategies.

show abstract

“…This is the case for sweet potato plants infected with Sweet potato chlorotic stunt virus (SPCSV, genus Crinivirus ) that show either absence of symptoms or mild symptoms, but when co-infected with members of the genera Potyvirus, Ipomovirus, Cucumovirus and Carlavirus develop severe diseases [21, 22]. Infections of cucumber by Cucurbit chlorotic yellows virus (CCYV) and Cucurbit yellow stunting disorder virus (CYSDV) criniviruses constitute another example: they cause symptoms in single infections that in co-infections with a Begomovirus become more severe, with crop yield reductions [23].…”

Section: Discussionmentioning

confidence: 99%

Molecular insights on potato yellow vein crinivirus infections in the highlands of Colombia

Niño

Toro

Tenllado

et al. 2021

Journal of General Virology

View full text Add to dashboard Cite

Potato yellow vein virus (PYVV) was detected in potatoes grown in the Central highlands, north of Bogotá (~3000 m altitude), Colombia. At this altitude viral whitefly vectors are largely absent, but infection persists because of the use of uncertified tubers. Plants with typical PYVV-induced yellowing symptoms, as well as with atypical yellowing or non-symptomatic symptoms were sampled at three separate geographical locations. PYVV presence was assessed by RT-PCR, and several plants were subjected to high-throughput sequencing (HTS) of their small RNA (sRNA) populations. Complete or almost complete sequences of four PYVV isolates were thus reconstructed, all from symptomatic plants. Three viral isolates infected plants singly, while the fourth co-infected the plant together with a potyvirus. Relative proportions of sRNAs to each of the three crinivirus genomic RNAs were found to remain comparable among the four infections. Genomic regions were identified as hotspots of sRNA formation, or as regions that poorly induced sRNAs. Furthermore, PYVV titres in the mixed versus single infections remained comparable, indicating an absence of synergistic/antagonistic effects of the potyvirus on the accumulation of PYVV. Daughter plants raised in the greenhouse from tubers of the infected, field-sampled plants displayed mild PYVV infection symptoms that disappeared with time, demonstrating the occurrence of recovery and asymptomatic infection phenotypes in this pathosystem.

show abstract

Co-infection of two criniviruses and a begomovirus enhances the disease severity in cucumber

Cited by 24 publications

References 27 publications

Sub-Lethal Effects of Partially Purified Protein Extracted from Beauveria bassiana (Balsamo) and Its Presumptive Role in Tomato (Lycopersicon esculentum L.) Defense against Whitefly (Bemisia tabaci Genn.)

Sub-Lethal Effects of Partially Purified Protein Extracted from Beauveria bassiana (Balsamo) and Its Presumptive Role in Tomato (Lycopersicon esculentum L.) Defense against Whitefly (Bemisia tabaci Genn.)

Criniviruses associated with cucurbit yellows disease in Greece and Cyprus: an ever‐changing scene

Molecular insights on potato yellow vein crinivirus infections in the highlands of Colombia

Contact Info

Product

Resources

About