Co-inoculum of Beauveria brongniartii and B. bassiana shows in vitro different metabolic behaviour in comparison to single inoculums

Canfora, Loredana; Abu-Samra, Nadir; Tartanus, Małgorzata; Łabanowska, B.H.; Benedetti, A.; Pinzari, Flavia; Malusà, Eligio

doi:10.1038/s41598-017-12700-0

Cited by 16 publications

(21 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We and others hope to accomplish these milestones with the recent availability of a high-quality P. destructans genome and data pipelines to automate Biolog analysis 15 , 17 – 20 .…”

Section: Discussionmentioning

confidence: 99%

Phenotype profiling of white-nose syndrome pathogen Pseudogymnoascus destructans and closely-related Pseudogymnoascus pannorum reveals metabolic differences underlying fungal lifestyles

Chaturvedi

DeFiglio

2018

F1000Res

View full text Add to dashboard Cite

Abstract, a psychrophile, causes bat Background: Pseudogymnoascus destructans white-nose syndrome (WNS)., a closely related Pseudogymnoascus pannorum fungus, causes human and canine diseases rarely. Both pathogens were reported from the same mines and caves in the United States, but only P.caused WNS. Earlier genome comparisons revealed that destructans P. contained more deduced proteins with ascribed enzymatic functions pannorum than . P. destructansWe performed metabolic profiling with Biolog PM microarray plates Methods: to confirm gene predictions. in silico utilized 78 of 190 carbon sources (41%), and 41 of 91 Results: P. pannorum nitrogen compounds (43%) tested.used 23 carbon compounds P. destructans (12%) and 23 nitrogen compounds (24%).exhibited more robust P. destructans growth on the phosphorous compounds and nutrient supplements (83% and 15%, respectively) compared to (27% and 1%, respectively.). P. pannorum P. exhibited higher tolerance to osmolytes, pH extremes, and a variety pannorum of chemical compounds than . P. destructans An abundance of carbohydrate degradation pathways combined Conclusions: with robust stress tolerance provided clues for the soil distribution of P.. The limited metabolic profile of was compatible with pannorum P. destructans predictions of far fewer proteins and enzymes.ability to in silico P. destructans catabolize diverse phosphorous and nutrient supplements might be critical in the colonization and invasion of bat tissues. The present study of 1,047 different metabolic activities provides a framework for future gene-function investigations of the unique biology of the psychrophilic fungi.

show abstract

“…We and others hope to accomplish these milestones with the recent availability of a high-quality P. destructans genome and data pipelines to automate Biolog analysis 15 , 17 – 20 .…”

Section: Discussionmentioning

confidence: 99%

Phenotype profiling of white-nose syndrome pathogen Pseudogymnoascus destructans and closely-related Pseudogymnoascus pannorum reveals metabolic differences underlying fungal lifestyles

Chaturvedi

DeFiglio

2018

F1000Res

View full text Add to dashboard Cite

show abstract

“…Then, there is the occurrence of the term "concentration", that may refer to the dose of specific microorganisms in the soil that may impact on the establishment and functioning of inoculative species in the host soil, or to the concentration of contaminants and toxic compounds to be (bio)-removed. Lastly, terms like "persistence" were observed, thus highlighting the emerging need of detecting the persistence and the fate of microbial inoculants in the soil since their application [11,31,35,74]. When looking at the green cluster, "degradation", "remediation," and "persistence" terms exhibited an increasing occurrence over time, thus suggesting to what extent the use of microbial inoculants is gaining successful results for environmental purposes and what is the emerging research trend that goes beyond the production and application of microbial inoculants.…”

Section: Research Term World Evolution and Their Citation Impactsmentioning

confidence: 99%

“…An enormous variety of microorganisms belonging to several taxa of fungi and bacteria closely associated with plants and rhizospheric soil can be utilized for the formulation of bioproducts for plant growth and protection. It can be noted that several terms, such as "bio-inocula" or "bioinoculant", have been commonly used to refer to biofertilizers or microbial-based products [29][30][31][32]. However, despite the considerable number of studies and evidence of beneficial microorganisms (PGPMs, plant growth-promoting bacteria (PGPB), biocontrol agents, and biostimulants), the application of microbial-based products or "microbial inoculants" is still limited and hindered by several factors [33,34].…”

Section: Introductionmentioning

confidence: 99%

Trends in Soil Microbial Inoculants Research: A Science Mapping Approach to Unravel Strengths and Weaknesses of Their Application

et al. 2021

View full text Add to dashboard Cite

Microbial inoculants are widely accepted as potential alternatives or complements to chemical fertilizers and pesticides in agriculture. However, there remains a lack of knowledge regarding their application and effects under field conditions. Thus, a quantitative description of the scientific literature related to soil microbial inoculants was conducted, adopting a science mapping approach to observe trends, strengths, and weaknesses of their application during the period of 2000–2020 and providing useful insights for future research. Overall, the study retrieved 682 publications with an increasing number during the 2015–2020 period, confirming China, India, and the U.S. as leading countries in microbial inoculants research. Over the last decade, the research field emphasized the use of microbial consortia rather than single strains, with increasing attention paid to sustainability and environmental purposes by means of multidisciplinary approaches. Among the emerging topics, terms such as “persistence” indicate the actual need for detecting and monitoring the persistence and fate of soil microbial inoculants. On the other hand, the low occurrence of terms related to failed studies as well as formulation processes may have limited the overall comprehension of the real potential of microbial inoculants to date. In conclusion, successful application of soil microbial inoculants in agriculture requires filling the fundamental knowledge gaps related to the processes that govern dynamics and interactions of the inoculants with soil and its native microbiota.

show abstract

“…Curve parameters offer the main advantage of being independent of incubation time, while also accounting for potential differential rates of colour development across substrates and plates. An example of this approach is given in the following work: Canfora et al (2017) 1 , and theory is reported in the references therein.The authors tested only one strain for each species (I agree that PM plates are very expensive, but drawing a result using only one strains in the comparison is a limitation in case of intraspecific variability, even if they used two replicates)The lowest value on the heat map is an OD of “zero”. How was data scaling performed?…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Phenotype profiling of white-nose syndrome pathogen Pseudogymnoascus destructans and closely-related Pseudogymnoascus pannorum reveals metabolic differences underlying fungal lifestyles

DeFiglio

Chaturvedi

2018

F1000Res

View full text Add to dashboard Cite

Background: Pseudogymnoascus destructans, a psychrophile, causes bat white-nose syndrome (WNS). Pseudogymnoascus pannorum, a closely related fungus, causes human and canine diseases rarely. Both pathogens were reported from the same mines and caves in the United States, but only P. destructans caused WNS. Earlier genome comparisons revealed that P. pannorum contained more deduced proteins with ascribed enzymatic functions than P. destructans. Methods: We performed metabolic profiling with Biolog PM microarray plates to confirm in silico gene predictions. Results: P. pannorum utilized 78 of 190 carbon sources (41%), and 41 of 91 nitrogen sources (43%) tested. P. destructans used 23 carbon compounds (12%) and 23 nitrogen compounds (24%). P. destructans exhibited more robust growth on the phosphorous sources and nutrient supplements (83% and 15%, respectively) compared to P. pannorum (27% and 1%, respectively.). P. pannorum exhibited higher tolerance to osmolytes, pH extremes, and a variety of chemical compounds than P. destructans. Conclusions: An abundance of carbohydrate degradation pathways combined with robust stress tolerance provided clues for the soil distribution of P. pannorum. The limited metabolic profile of P. destructans validated in silico predictions of far fewer proteins and enzymes. P. destructans ability to catabolize diverse phosphorous and nutrient supplements might be critical in the colonization and invasion of bat tissues. The present study of 1,047 different metabolic activities provides a framework for future gene-function investigations of the unique biology of the psychrophilic fungi.

show abstract

Co-inoculum of Beauveria brongniartii and B. bassiana shows in vitro different metabolic behaviour in comparison to single inoculums

Cited by 16 publications

References 76 publications

Phenotype profiling of white-nose syndrome pathogen Pseudogymnoascus destructans and closely-related Pseudogymnoascus pannorum reveals metabolic differences underlying fungal lifestyles

Phenotype profiling of white-nose syndrome pathogen Pseudogymnoascus destructans and closely-related Pseudogymnoascus pannorum reveals metabolic differences underlying fungal lifestyles

Trends in Soil Microbial Inoculants Research: A Science Mapping Approach to Unravel Strengths and Weaknesses of Their Application

Phenotype profiling of white-nose syndrome pathogen Pseudogymnoascus destructans and closely-related Pseudogymnoascus pannorum reveals metabolic differences underlying fungal lifestyles

Contact Info

Product

Resources

About