Co-Metabolic Degradation of β-Cypermethrin and 3-Phenoxybenzoic Acid by Co-Culture of Bacillus licheniformis B-1 and Aspergillus oryzae M-4

Zhao, Jianxin; Chi, Yuanlong; Xu, Yingchao; Jia, Dongying; Yao, Kai

doi:10.1371/journal.pone.0166796

Cited by 37 publications

(34 citation statements)

References 26 publications

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“…The bacterium successfully grows by using and converting this toxic insecticide into non-toxic metabolic compounds. Similar results have been observed with different strains of bacteria and fungi [59]. Several degradation mechanisms for cypermethrin and other pyrethroids have been identified in bacterial strains of Stenotrophomonas, Acinetobacter, Bacillus, Raoultella, Pseudomonas, and Brevibacterium, and fungal strains Aspergillus, Candidia, and Trichoderma [2,15,19,20,[59][60][61].…”

Section: Discussionsupporting

confidence: 84%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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Cypermethrin is popularly used as an insecticide in households and agricultural fields, resulting in serious environmental contamination. Rapid and effective techniques that minimize or remove insecticidal residues from the environment are urgently required. However, the currently available cypermethrin-degrading bacterial strains are suboptimal. We aimed to characterize the kinetics and metabolic pathway of highly efficient cypermethrin-degrading Bacillus thuringiensis strain SG4. Strain SG4 effectively degraded cypermethrin under different conditions. The maximum degradation was observed at 32 °C, pH 7.0, and a shaking speed of 110 rpm, and about 80% of the initial dose of cypermethrin (50 mg·L−1) was degraded in minimal salt medium within 15 days. SG4 cells immobilized with sodium alginate provided a higher degradation rate (85.0%) and lower half-life (t1/2) of 5.3 days compared to the 52.9 days of the control. Bioaugmentation of cypermethrin-contaminated soil slurry with strain SG4 significantly enhanced its biodegradation (83.3%). Analysis of the degradation products led to identification of nine metabolites of cypermethrin, which revealed that cypermethrin could be degraded first by cleavage of its ester bond, followed by degradation of the benzene ring, and subsequent metabolism. A new degradation pathway for cypermethrin was proposed based on analysis of the metabolites. We investigated the active role of B. thuringiensis strain SG4 in cypermethrin degradation under various conditions that could be applied in large-scale pollutant treatment.

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Section: Discussionsupporting

confidence: 84%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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“…Although metabolic mechanism is the basis of application, reports on co-culture biodegradation pathway and mechanism are still rare (Chen et al 2012b;Zhao et al 2016). This research veri ed the degradation mechanism of deltamethrin and 3-PBA by co-culture of strains LH-1-1 and BPBA052 ( Fig.…”

Section: Discussionmentioning

confidence: 69%

“…This may be due to that strain LH-1-1 cannot quickly and completely degrade toxic intermediate 3-PBA, while BPBA052 possess highe cient degradation ability in 3-PBA. Therefore, when a proper amount of BPBA052 was introduced, the toxicity inhibition of 3-PBA was quickly released in the degradation process, and the degradation of deltamethrin was further accelerated (Zhao et al 2016). Liu et al (2014) reported that the appropriate proportion of Bacillus licheniformis B-1 and Sphingomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…However, most of these microorganisms cannot completely mineralize pesticides or their metabolites alone (Chen et al 2012b). Zhao et al (2016) reported that about 50% β-cypermethrin (100 mg/L) could be degraded by Bacillus licheniformis B-1 after 72 h, but was unable to sustain mineralization of 3-PBA; Zhu et al (2016) reported that 80.62% 3-PBA (100 mg/L) could be degraded by Aspergillus oryzae M-4 within 5 d, but did not clear its ability to degrade PPs. Therefore, a new idea emerged, wherein synergy between strains to simultaneously degrade pyrethroid pesticides and their toxic intermediate metabolites (Jones et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Compared with other biodegradation methods, in co-cultures, microbes are more adaptable to the environment and can achieve more rapid pathway optimization, thereby improving the e ciency of pollutant degradation (Zhao et al 2016;Jones et al 2018). Recently, some reports have veri ed that microbial co-cultures can effectively degrade organic pollutants and their related metabolites (Zhao et al 2016;Tran et al 2013). Liu et al (2014) studied the co-degradation of cypermethrin and 3-PBA by strains Bacillus licheniformis B-1 and Sphingomonas sp.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of deltamethrin degradation and metabolic pathway by co-culture of Acinetobacter junii LH-1-1 and Klebsiella pneumoniae BPBA052

Tang

Lei

et al. 2020

Preprint

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Deltamethrin and its major metabolite 3-phenoxybenzoic acid (3‐PBA) have caused serious threat to the environment as well as human health, yet little is known about their degradation pathways by bacterial co-cultures. In this study, the growth and degradation kinetics of Acinetobacter junii LH-1-1 and Klebsiella pneumoniae BPBA052 during deltamethrin and 3-PBA degradation were established, respectively. When the inoculum proportion of the strains LH-1-1 and BPBA052 was 7.5:2.5, and LH-1-1 was inoculated 24 h before inoculation of strain BPBA052, 94.25% deltamethrin was degraded and 9.16 mg/L of 3-PBA remained within 72 h, which was 20.36% higher and 10.25 mg/L lesser than that in monoculture of LH-1-1, respectively. And the half-life of deltamethrin was shortened from 38.40 h to 24.58 h. Based on gas chromatography–mass spectrometry, 3-phenoxybenzaldehyde, 1,2-benzenedicarboxylic butyl dacyl ester, and phenol were identified as metabolites during deltamethrin degradation in co-culture. This is the first time that a co-culture degradation pathway of deltamethrin has been proposed based on these identified metabolites. Bioremediation of deltamethrin-contaminated soils with co-culture of strains LH-1-1 and BPBA052 significantly enhanced deltamethrin degradation and 3-PBA removal. This study provides a platform for further studies on deltamethrin and 3-PBA biodegradation mechanism in co-culture, and it also proposes a promising approach for efficient bioremediation of environment contaminated by pyrethroid pesticides and their associated metabolites.

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Pyrethroid-Degrading Microorganisms and Their Potential Application for the Bioremediation of Contaminated Environments

Huang

Chen

2022

Enzymes for Pollutant Degradation

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Co-Metabolic Degradation of β-Cypermethrin and 3-Phenoxybenzoic Acid by Co-Culture of Bacillus licheniformis B-1 and Aspergillus oryzae M-4

Cited by 37 publications

References 26 publications

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Characterization of deltamethrin degradation and metabolic pathway by co-culture of Acinetobacter junii LH-1-1 and Klebsiella pneumoniae BPBA052

Pyrethroid-Degrading Microorganisms and Their Potential Application for the Bioremediation of Contaminated Environments

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