2012
DOI: 10.1083/jcb.201204149
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Co-operation between different targeting pathways during integration of a membrane protein

Abstract: The Sec and Tat pathways are both required to insert the three hydrophobic domains of the Rieske protein into the membrane.

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Cited by 45 publications
(83 citation statements)
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“…Double-crossover mutants were selected by screening for hygromycin resistance and kanamycin sensitivity. Deletion of qcrCAB was confirmed by PCR analysis of chromosomal DNA using oligonucleotides 50-48seq For (5=-CGGCCGCACCTACGCGGCCCGGAGGTTCACCC ACG) and 50-48seq Rev (5=-CGGGGCCCTTCGACCGTGGTGTGGCG ACGCTGGAGGG-3=) that lie outside the deleted region and, additionally, by Western blot analysis using a QcrA polyclonal antibody (12). The ⌬qcrCAB::hyg strain was subsequently designated APH2.…”
Section: Methodsmentioning
confidence: 99%
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“…Double-crossover mutants were selected by screening for hygromycin resistance and kanamycin sensitivity. Deletion of qcrCAB was confirmed by PCR analysis of chromosomal DNA using oligonucleotides 50-48seq For (5=-CGGCCGCACCTACGCGGCCCGGAGGTTCACCC ACG) and 50-48seq Rev (5=-CGGGGCCCTTCGACCGTGGTGTGGCG ACGCTGGAGGG-3=) that lie outside the deleted region and, additionally, by Western blot analysis using a QcrA polyclonal antibody (12). The ⌬qcrCAB::hyg strain was subsequently designated APH2.…”
Section: Methodsmentioning
confidence: 99%
“…Unlike most other bacteria, there is no gene encoding a soluble cytochrome c in the genomes of S. coelicolor, M. tuberculosis, or Corynebacterium glutamicum (10). Finally the Rieske iron-sulfur protein (QcrA) is much more hydrophobic than previously characterized, monotopic Rieske proteins (8,11) and has three TMD preceding the globular iron-sulfur clustercontaining domain (12) (Fig. 1B).…”
mentioning
confidence: 94%
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“…OPH Is a Lipoprotein-The Tat system is known to assemble some membrane-anchored proteins (20,35,36). In bacteria these usually have either a single transmembrane domain at the C terminus (35) or an N-terminal non-cleaved twin-arginine signal anchor sequence (37).…”
Section: Oph Is Tightly Bound To the B Diminuta Inner Membrane-mentioning
confidence: 99%
“…1). Apart from QcrA of B. subtilis, other Rieske proteins also share a strong association with the Tat pathway as was demonstrated in various bacteria and chloroplasts of green plants (43)(44)(45)(46)(47). Notably, the Gram-negative bacterium Escherichia coli does not contain a cytochrome bc 1 complex, which precludes studies on Rieske protein assembly in this important model organism for research on Tat-dependent protein translocation (32,33).…”
mentioning
confidence: 99%