Co-overexpression of AVP1 and OsSIZ1 in Arabidopsis substantially enhances plant tolerance to drought, salt, and heat stresses

Abstract

Abiotic stresses such as water deficit, salt, and heat are major environmental factors that negatively affect plant growth, development, and productivity. Previous studies showed that overexpression of the Arabidopsis vacuolar H + -pyrophosphatase gene AVP1 increases salt and water deficit stress tolerance and overexpression of the rice SUMO E3 ligase gene OsSIZ1 improves heat and water deficit stress tolerance in transgenic plants. In this report, the effects of co-overexpression of AVP1 and OsSIZ1 in Arabidopsis on abiotic stress tolerance were studied. It was found that AVP1/OsSIZ1 co-overexpressing plants performed significantly better than AVP1 -overexpressing plants and OsSIZ1 -overexpressing plants, and produced 100% more seed than wild-type plants under single stress or multiple stress conditions. The increased stress tolerance in AVP1/OsSIZ1 co-overexpressing plants was substantially larger than the increased stress tolerance in AVP1 -overexpressing plants and OsSIZ1 -overexpressing plants under every abiotic stress condition tested. This research provides the proof-of-concept that crop yields might be substantially improved using this approach.

Recurrent Selection by Herbicide Sublethal Dose and Drought Stress Results in Rapid Reduction of Herbicide Sensitivity in Junglerice

“…In addition, HSPs are known as stress-responsive proteins, as their concentration increases rapidly in plant cells in response to environmental stresses [79,80]. Currently, many research groups are investigating the mechanisms of action or function of HSPs in plants under various abiotic stress-causing conditions such as drought, salt, cold, heat, and even herbicides [81][82][83][84][85][86]. As expected, we determined that HSP transcript levels are higher under combined drought and herbicide stresses.…”

Towards doubling fibre yield for cotton in the semiarid agricultural area by increasing tolerance to drought, heat and salinity simultaneously

“…The Arabidopsis vacuolar H + ‐pyrophosphatase gene AVP1 and the rice SUMO E3 ligase gene OsSIZ1 were fused to Cauliflower mosaic virus 35S promoter and a maize ubiquitin promoter, respectively, with the nopaline synthase terminator sequence used in both expression cassettes. Then, these two expression cassettes were inserted into the T‐DNA region of the pBI121‐based binary vector to form the transforming vector (Esmaeili et al ., 2019), which was used to transform wild‐type (WT) cotton. A total of 27 independent transgenic cotton plants were generated via Agrobacterium‐mediated transformation method (Bayley et al ., 1992).…”

“…Therefore, engineering crops that can maintain high yield in marginal environments would be a major break‐through in agricultural production. Recently our proof‐of‐concept study showed that co‐overexpression of AVP1 and OsSIZ1 in Arabidopsis improves multi‐stress tolerance substantially, and transgenic plants produced a significantly higher yield under severe stress conditions (Esmaeili et al ., 2019). In this study, we co‐overexpressed OsSIZ1 and AVP1 in cotton, and we showed that OsSIZ1/AVP1 co‐overexpressing cotton plants demonstrated a far more superior performance than all other control plants under all abiotic stress conditions tested.…”

Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier

“…8 It is unclear whether different gene expression cassettes produce similar levels of mRNA molecules in the same plant tissue. 9 Individual mRNA molecules can correspond to numerous proteins that interact each other and might not behave similarly in a single plant cell. [10][11][12] Furthermore, multiple proteins participating in the same event may have distinct vulnerabilities to protein degradation over the duration of an experiment.…”