Co-production of carbonic anhydrase and phycobiliproteins by Spirulina sp. and Synechococcus nidulans

Ores, Joana da Costa; Amarante, Marina Campos Assumpção de; Kalil, Susana Juliano

doi:10.1016/j.biortech.2016.07.133

Cited by 51 publications

(25 citation statements)

References 43 publications

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“…Although it is a chemical–physical method of extraction, in which the cell rupture and extraction steps occur concomitantly, it was chosen because it is possible to use wet biomass to obtain C‐PC. In addition, this method provides high efficiency in terms of C‐PC yield and short treatment times as demonstrated by Ores et al…”

Section: Resultsmentioning

confidence: 89%

“…PEF‐assisted extraction of C‐PC led to an extraction yield of 159.9 mg/g and purity of 0.46 . UAE of C‐PC obtained extraction yields of 86.3, 35.5, and 90.0 mg/g whereas purities were 1.32 and 0.65 . Four successive repeated freezing–thawing allowed the obtainment of purity around 1.4 .…”

Section: Resultsmentioning

confidence: 98%

“…C‐PC extraction from other strains of Spirulina by different extraction techniques has been reported in the literature . PEF‐assisted extraction of C‐PC led to an extraction yield of 159.9 mg/g and purity of 0.46 .…”

Section: Resultsmentioning

confidence: 99%

“…Cultivation was performed on discontinuous mode using a photobioreactor (Erlenmeyer flask), where 20% (v/v) of inoculum (initial concentration of 0.75 g/L) was added . Temperature, agitation/aeration, and light were performed according to Ores et al Cells were recovered by filtration through a cellulose acetate membrane and destined for the cell pretreatment step and subsequent extraction process.…”

Section: Methodsmentioning

confidence: 99%

“…In order to obtain the dye, the cell must be ruptured before extraction. Several cell rupture and C‐PC extraction methods from Spirulina have been reported, such as extraction assisted by pulsed electric field (PEF), freeze‐thawing, extraction assisted by mechanical homogenizer, ultrasound‐assisted extraction (UAE), ultrasonic bath extraction, microwave‐assisted extraction (MAE), milling, and supercritical fluid extraction, or the combination of these methods . C‐PC extraction yields of these methods ranged from 29.1 to 194 mg/g while purities varied from 0.46 to 1.4 .…”

Section: Introductionmentioning

confidence: 99%

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Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Sala

Moraes

Kalil

2018

Biotechnology Progress

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C‐phycocyanin (C‐PC) is a natural blue dye, and depending on its purity, which is measured by the ratio between the absorbance of the chromophore (A620) and the absorbance of the proteins (A280), it can be used in food (purity > 0.7), cosmetics (purity > 1.5), and therapeutic treatments (purity > 4.0). Several physical, chemical, and enzymatic methods of extraction are reported, however, few are able to extract C‐PC with purity above 0.7. An innovative method of C‐PC extraction with food grade purity from wet Spirulina platensis biomass is proposed. The cells were pretreated with ethylenediaminetetraacetic acid and subsequent C‐PC extraction was performed with tris‐(hydroxymethyl) aminomethane‐SO4 buffer. C‐PC was released after 12 h of cell pretreatment. Six variables of the extraction process were evaluated. The extraction temperature significantly influenced C‐PC extraction yield and purity. In the best condition of cell pretreatment and extraction, C‐PC with purity of 1.0 and extraction yield of 129.0 mg/g could be obtained to be used as a food dye without any purification process. Lastly, an ultrafiltration process was integrated and C‐PC was concentrated 8.8‐fold, resulting in purity of 1.6 and recovery of 93.4%.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Sala

Moraes

Kalil

2018

Biotechnology Progress

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Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

et al. 2018

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The simultaneous production of carbonic anhydrase and the phycobiliproteins, C-phycocyanin and allophycocyanin, was evaluated during the cultivation of Spirulina platensis using Zarrouk's medium in its non-diluted (100 % ZM) and diluted to 20 % (v/v) (20 % ZM) forms. In both media, Spirulina simultaneously produced carbonic anhydrase and phycobiliproteins. For the phycobiliproteins' production, higher concentrations (mg/g) of C-phycocyanin and allophycocyanin were obtained when 100 % ZM was used. For carbonic anhydrase production, higher concentrations (U/g) were obtained on the 6 th day of cultivation in both culture media. 100 % ZM promoted greater productions of these bioproducts, and, for simultaneous obtainment, the cells should be harvested after 21 days, obtaining concentrations of 137.4 mg/g C-phycocyanin, 104.4 mg/g allophycocyanin, and 26.6 U/g carbonic anhydrase.

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Comparative growth characteristics and interspecific competitive interaction of two cyanobacteria, Phormidium autumnale and Nostoc sp.

2021

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This study examined the growth characteristics and competitive interaction of two cyanobacteria, Phormidium autumnale GJ_2B_I1 and Nostoc sp. DS_2B_I1, which were newly isolated from a southeast river (Nakdong) during the cyanobacterial harmful algal bloom (CyanoHAB) season in Korea. As major environmental parameters, water temperature (25 and 30 °C) and alkalinity (19–78 mg CaCO3 L–1) and nitrate concentration (1.5–3.5 mg NO3–N L–1) were selected based on the water environmental monitoring data during the CyanoHAB season. Unlike P. autumnale, Nostoc sp. has a relatively high growth rate under both monoculture and co‐culture and prefers the maximum environmental conditions (30 °C and 78 mg CaCO3 L–1; pH 9) during the CyanoHAB season. In addition, the growth of P. autumnale is relatively unaffected by alkalinity. Nitrogen (N) stress also has a limiting effect in the interspecific interactions of both cyanobacterial strains. All other cases except for Nostoc sp. in a co‐culture showed a considerable increase in growth rate with increasing N content (1.5–3.5 mg NO3–N L–1), showing 20–64% under the minimum field conditions (25 °C and 19 mg CaCO3 L–1; pH 7) and 18–140% under the maximum field conditions. The results show that the growth of P. autumnale can be stimulated by enhanced N stress. On the other hand, Nostoc sp. is less affected by N stress compared with P. autumnale. Therefore, it has excellent potential to be a major group of CyanoHABs because of their relatively high growth rate, particularly in the range of N tested.

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Co-production of carbonic anhydrase and phycobiliproteins by Spirulina sp. and Synechococcus nidulans

Cited by 51 publications

References 43 publications

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Simultaneous production of phycobiliproteins and carbonic anhydrase by Spirulina platensis LEB‐52

Comparative growth characteristics and interspecific competitive interaction of two cyanobacteria, Phormidium autumnale and Nostoc sp.

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