Co-regulation Role of Endogenous Hormones and Transcriptomics Profiling Under Cold Stress in Tetrastigma hemsleyanum

Abstract

Background: Tetrastigma hemsleyanum Diels et Gilg is a valuable medicinal herb, Chilling sensitivity is the dominant environmental factor limiting the artificial domestication of the plant. Hormonerelated gene regulation and hormone signaling pathways in response to cold stress in T. hemsleyanum remain unknown. Results: Some key genes involved in hormones biosynthesis, such as ZEP and NCED genes of ABA biosynthesis, GA2ox, GA3ox, and GA20ox genes of GA biosynthesis, ACO genes of ET biosynthesis pathway were screened to be crucial in cold response. Consistently, the response of ABA and ABA/GA1+3 to cold stress was prior to that of GA1+3, ZR, ABA/IAA, and ABA/ZR. The increasing changes in ABA/GA1+3 turned to a steep decline with the extension of stress time, which might be one factor contributing to cold-sensitivity of T. hemsleyanum under prolonged stress. The cold tolerance of T. hemsleyanum would be enhanced by ABA but repressed by GA3 when each phytohormone was applied alone. The ABA-mediated promotion and GA-mediated repression of cold tolerance could both be attenuated by the co-application of the two phytohormones within 6h. When the biosynthesis of endogenous ABA and GA were inhibited by FLU and PAC, respectively, the effects of GA and ABA treatment were reversed partially. Conclusions: In summary, we presented the first study of global expression patterns of hormoneregulated transcripts in T. hemsleyanum. This study suggested that GA and ABA could work antagonistically to balance the responses to cold in T. hemsleyanum. PAC, a GA biogenesis inhibitor, as well as exogenous ABA, might be potential plant growth regulators that can promote cold tolerance of T. hemsleyanum. The study also provided valuable hints in revealing a new theoretical basis and potential candidate genes that govern cold tolerance of T. hemsleyanum.

Full-length transcriptome reveals the pivotal role of ABA and ethylene in the cold stress response of Tetrastigma hemsleyanum

“…The 9-cis epoxycarotenoid dioxygenase (NCED) is considered to be a key rate-limiting enzyme in ABA biosynthesis ( González-Silva et al., 2011 ); previous research has shown that cold stress caused significant upregulation of NCED s at 24 h in T. hemsleyanum ( Peng et al., 2021 ). However, in this research study, two out of three NCEDs were suppressed by cold, and one out of three NCEDs were only significantly induced in FS.…”

Transcriptome and Metabonomics Combined Analysis Revealed the Defense Mechanism Involved in Hydrogen-Rich Water-Regulated Cold Stress Response of Tetrastigma hemsleyanum

“…RNA extraction, quantification, and transcriptome sequencing were carried out according to our previous method ( Peng et al, 2019 , 2021 ). We used NEBNext ® Ultra™ RNA Library Prep Kit for Illumina ® (NEB, United States) to generate sequencing libraries.…”

Integrated transcriptomics and metabolomics analysis reveals key regulatory network that response to cold stress in common Bean (Phaseolus vulgaris L.)

“…Cold treatment induces many biochemical pathways, including glucose metabolism [ 7 ] and amino acid metabolism [ 8 ], which are important for the synthesis of not only proteins but also precursors of various metabolites and metabolic products with multiple functions in plant responses to cold. Furthermore, studies have revealed that the regulation of plant hormones, including abscisic acid (ABA) [ 9 , 10 ], gibberellic acid (GA) [ 11 ], and jasmonic acid (JA) [ 12 ], changes during cold stress.…”