Co-SLD suppressed the growth of oral squamous cell carcinoma via disrupting mitochondrial function

Abstract

To evaluate the safety and efficacy of novel cobalt complex with sulindac (Co-SLD), the zebrafish and oral squamous cell carcinoma CAL27 were investigated in the present study. The developmental toxicity of Co-SLD ranging from 5 to 20 lM was determined by exposure to 3-144-h post-fertilization (hpf) zebrafish. Our data showed that Co-SLD did not cause to the appreciable toxicity at low concentration (5 and 10 lM). A remarkable toxicity was observed at high concentration (20 lM), including increased mortality and malformation, delayed hatchability, reduced heart rate as well as suppressed behaviour. With regard to the antitumor activity of Co-SLD, inhibited cell growth and migration capability were outstandingly observed in oral squamous cell carcinoma treated with 10 and 20 lM Co-SLD, which could be mainly attributed to the Co-SLD-elicited mitochondrial damage as marked by the depression of mitochondrial membrane potential, ROS accumulation and ATP depletion. Furthermore, administration of 10 lM Co-SLD was an optimal concentration not only to avoid the normal tissue toxicity, but also to enhance the killing of cancer cells via disrupting mitochondrial dysfunction. Taken together the above results demonstrated the desirable response of oral squamous cell carcinoma to Co-SLD. ARTICLE HISTORY

Radiosensitivity enhancement by Co‐NMS‐mediated mitochondrial impairment in glioblastoma

“…Moreover, the frequency of tail contraction in 20 μM Co‐NMS‐exposed group was prominently reduced by 71.4% than that of in the control group (Figure 3b). These data suggested that the higher doses of Co‐NMS dramatically led to the behavioral abnormality at the earliest stages, which were in line with our previous outcomes (S. Li et al, 2019).…”

“…The zebrafish (AB strain) were kept in our standard laboratory procedures (S. Li et al, 2019). Human U251 glioma cells were purchased from the BeNa Culture Collection (Beijing, China), and grown in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Hyclone, Logan, UT) under 37°C of 5% CO 2 condition.…”