CO₂-responsive Pickering emulsions stabilized by soft protein particles for interfacial biocatalysis

Abstract

Pickering emulsions are emulsions stabilized by colloidal particles and serve as an excellent platform for biphasic enzymatic catalysis. However, developing simple and green strategies to avoid enzyme denaturation, facilitate product...

Multifunctional integrated compartment systems for incompatible Pickering interfacial catalysis cascade reactions based on responsive core–shell nanoparticles

“…We recently discovered a switchable self-assembly behavior of an intrinsically disordered protein, NaCas, which was used as a robust emulsifier for shaping unprecedented CO 2 /N 2 - or pH-responsive emulsions. 9,21,22 The obtained emulsion had infinitely switchable cyclability and afforded a quick and facile demulsification process, even in complicated environments ( e.g. , a saturated NaCl solution).…”

“…Based on our previous studies, 9,21,22 we have evolved material design strategies to overcome this technical challenge. 9,12 Furthermore, this protein (Sodium Caseinate) readily adsorbs onto the mesoporous nanoparticle surfaces and imparts the resulting composite structures with various functionalities, such as potent emulsification and responsiveness.…”

A facile approach to obtain super-hydrophobicity for cotton fiber fabrics

“…[27][28][29] Substituting solid particles for traditional surfactants, Pickering emulsions are more stable against coalescence and can obtain many useful properties. [30][31][32] Among the present methods to build controllable Pickering emulsions, tuning the amphiphilicity of particles is comparatively effective and has attracted enormous attention. [33][34][35] Here, we synthesis a kind of thermosensitive microgel to be used as Pickering emulsier, where the PNIPAm acts as thermosensitive and hydrophobic part and the PEI acts as a hydrophilic part.…”

Lipase-Entrapped Colloidosomes with Tunable Positioning at the Oil–Water Interface for Pickering Emulsion-Enhanced Biocatalysis

“…Enzymes are the cornerstone for biocatalysis, providing necessary compounds (fine chemicals, pharmaceuticals, and fuel) for humans with unparalleled activity, selectivity, and turnover numbers. − However, biocatalysis in the industry usually involves immiscible biphasic systems; the limited interfacial area of the biphasic system extremely restricts the reaction rate. − Fortunately, Pickering interfacial biocatalysis (PIB) based on the interface-active enzyme-loading particle (Pickering interfacial biocatalyst) provides a powerful platform for efficient enzymatic catalysis in the biphasic system. − Pickering interfacial biocatalysts assembled at the oil–water interface are able to stabilize the Pickering emulsions, enlarge the reaction interface area, and shorten the mass transfer distance to enhance biocatalysis in the biphasic system. − However, Pickering interfacial biocatalysts located on the oil–water interface still face the inevitable deactivation when one of the phases contains the reactant that inactivates the enzyme, such as the short-chain alcohol in the enzymatic biodiesel production, − because the Pickering interfacial biocatalysts at the Pickering emulsion interface protrude partly into the oil phase (organic phase) and partly into the water phase. ,, The positive effects of PIB in improving the efficiency of enzymatic biodiesel production have been widely validated, ,− but the inevitable deactivation of the enzyme on the interface is still the crucial factor that hinders further improvement of the reaction rate and yield of enzymatic biodiesel production. , Therefore, an ideal Pickering interfacial biocatalyst should possess interfacial activity to provide a stable Pickering emulsion and should feature tunable positioning at the oil–water interface to reduce the deactivation of the enzyme caused by the reactant.…”