Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains

Abstract

Background: Fermentation of lignocellulosic biomass is an attractive alternative for the production of bioethanol. Traditionally, the yeast Saccharomyces cerevisiae is used in industrial ethanol fermentations. However, S. cerevisiae is naturally not able to ferment the pentose sugars D-xylose and L-arabinose, which are present in high amounts in lignocellulosic raw materials.

Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

“…Functional integration of a highly efficient d-xylose fermentation pathway with pathways that are under devel-opment (e.g. arabinose [9,36]) or under consideration (e.g. rhamnose [69]) therefore presents an additional challenge in metabolic engineering for efficient fermentation of plant biomass hydrolysates.…”

Perfis De Assimilação De Carboidratos De Uma Comunidade De Leveduras Associadas Ao Caldo De Cana De Açúcar

“…KNOSHAUG et al (2009) Segundo MARTINI (2014), a produção eficiente de etanol a partir de substratos hemicelulósicos exige que a levedura tenha a capacidade de utilizar não apenas a glicose, mas também os outros açúcares, como a arabinose. Assim, a incapacidade de assimilação especificamente da arabinose e da celobiose pela Saccharomyces cerevisiae (YOUNG et al, 2010;SUBTIL & BOLES, 2011) é um fator limitante para o uso da biomassa celulósica na produção de etanol (BECKER & BOLES, 2003;RICHARD et al, 2003;KARHUMAA et al, 2006).…”

Next-generation cellulosic ethanol technologies and their contribution to a sustainable Africa

“…E2 was further metabolically engineered to allow anaerobic growth on xylose in synthetic media [32]. Laboratory and industrial S. cerevisiae strains were also engineered to co-ferment the pentose sugars D-xylose and L-arabinose [31]. There have been many reports detailing the expression of one or more cellulase-encoding genes in S. cerevisiae [8].…”

“…However, this yeast has a number of shortcomings in terms of a CBP-processing organism, such as its inability to hydrolyse cellulose and hemicellulose or use xylose or arabinose. A number of research groups around the world have been working on improving the substrate range of S. cerevisiae to include the monomeric forms of sugars contained in plant biomass [15,22,28,31]. An S. cerevisiae strain that expressed the xylose isomerase gene from the fungus Piromyces sp.…”