The three marine microalgae (Chlorella vulgaris, Spirulina platensis and Nannochloropsis bacillaris) were collected from Vellar estuary, South east coast of India. These three microalgae were cultivated in respective media and estimated their biochemical composition and antibacterial activity. Simultaneously, these cultures were cultivated in flask containing 500 ml of respective media at lab condition for a period of one month and their growth, pH, biomass and CO 2 fixation and carbon content were determined. Based on the growth rate, the pH of three microalgae in media was observed at lab condition. During maximum growth and biomass, the pH was found to be ranged between 9 & 11 for Spirulina platensis, 7 & 9 for Chlorella vulgaris; 8 & 9 for Nannochloropsis bacillaris. Spirulina platensis and Chlorella vulgaris reached maximum growth rate whereas Chlorella vulgaris and Spirulina platensis showed maximum biomass produced. Chlorella vulgaris and Spirulina platensis attained maximum biomass in media at lab condition, also fixed highest level of carbon dioxide in media but they did not produce maximum biomass, though the growth of Nannochloropsis bacillaris were found high in media at lab condition. Among the three microalgae, Chlorella vulgaris and Spirulina platensis produced highest biochemical compounds. Hence Chlorella vulgaris and Spirulina platensis were selected as efficient microalgae for antibacterial activity against human pathogen. This study revealed that certain green algae and blue green microalgae having high growth, pH, CO 2 fixation, carbon content and biochemical composition paves the way for pharmaceutical activity. Antibacterial activity was evaluated for Chlorella and Spirulina with their potential health benefits.