CoA transferase in the brain and other mammalian tissues

Comparative studies on 3-oxo acid coenzyme A transferase from various rat tissues

“…The results indicate that the transferase from these tissues, with the possible exception of the skeletal muscle enzyme, is not localized in the cytoplasm. Considerable activity, comparable with that reported here, has been observed in heart and muscle cytoplasmic fractions (Tildon & Sevdalian, 1972;Lowenstein, 1967). However, our data for the heart Vol.…”

“…The total CoA transferase activity in kidney, heart, brain, skeletal muscle and liver was found to be slightly dependent on the homogenizing media (Table 1), but the activities were comparable with and in the same order as reported values Tildon & Sevdalian, 1972;Bassler et al, 1973). Similar results were obtained if the activity was measured in the reverse direction (i.e., the conversion of acetoacetate into the CoA derivative) or if the results were expressed in terms of mg of protein in the total sonicated material instead of g wet wt.…”

“…The addition of deoxycholate (0.3% w/v) to the phosphate medium or of Triton X-100 (0.1 and 0.3%) to the phosphate or Trissucrose medium neither increased the yield of activity nor the stability of the enzyme. The freezing-thawing technique for homogenization (Tildon & Sevdalian, 1972) gave consistently lower activities than the procedures described in the Materials and Methods section. The results indicate that no unusual effects on enzyme activity result from using the Tris-sucrose solution.…”

“…This enzyme has been found to have the highest activity in heart and kidney tissue of various mammals, whereas the activity is lowest in liver . CoA transferase clearly plays a role in permitting ketone bodies to serve as fuels for respiration (Krebs, 1961); however, its reported presence in the cytoplasmic fraction of certain tissue homogenates suggests a possible function in cellular biosynthetic activities (Tildon & Sevdalian, 1972;Lowenstein, 1967). Further, the different kinetic (Ki) properties of CoA transferase from pig heart and bovine skeletal muscle have been used to explain possible differences in tissue consumption of the ketone bodies (Blair, 1969).…”

Substrate Utilization and Brain Development

“…2), Tildon and Sevdalian (1972) reported no detect able activity in dog or calf brain. Although from the scheme outlined in Fig.…”