Coaggregation between and among human intestinal and oral bacteria

Ledder, Ruth G.; Timperley, Andrea S.; Friswell, Melissa K.; Macfarlane, Sandra; McBain, Andrew J.

doi:10.1111/j.1574-6941.2008.00525.x

Cited by 55 publications

(44 citation statements)

References 39 publications

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“…Mucin is a glycosylated protein and attachment of bacteria to mucin involves specific bacterial proteins such as mucus-binding proteins and other lectins or adhesins. A majority of the aggregation interactions between gut isolates are mediated by lectin-carbohydrate interaction (Ledder et al, 2008). In Lactobacillus reuteri strains, mucus binding showed excellent correlation with the presence of cell-surface mucus-binding protein and was further highly associated with the autoaggregation properties of Lb.…”

Section: Discussionmentioning

confidence: 89%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

113

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Bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. In the present study, two probiotic strains, Lactobacillus rhamnosus CNCM-I-3698 and Lactobacillus farciminis CNCM-I-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference Lactobacillus strains (Lb. rhamnosus GG and Lb. farciminis CIP 103136). The autoaggregation potential of both forms was higher than that of reference strains and twice that of pathogenic strains. The coaggregation potential of these two beneficial micro-organisms was evaluated against several pathogenic agents that threaten the global safety of the feed/food chain: Escherichia coli, Salmonella spp., Campylobacter spp. and Listeria monocytogenes. The strongest coaggregative interactions were demonstrated with Campylobacter spp. by a coaggregation test, confirmed by electron microscopic examination for the two forms. Viable forms were investigated for the nature of the bacterial cell-surface molecules involved, by sugar reversal tests and chemical and enzymic pretreatments. The results suggest that the coaggregation between both probiotic strains and C. jejuni CIP 70.2 T is mediated by a carbohydrate-lectin interaction. The autoaggregation potential of the two probiotics decreased upon exposure to proteinase, SDS or LiCl, showing that proteinaceous components on the surface of the two lactobacilli play an important role in this interaction. Adhesion abilities of both Lactobacillus strains were also demonstrated at significant levels on Caco-2 cells, mucin and extracellular matrix material. Both viable and heat-killed forms of the two probiotic lactobacilli inhibited the attachment of C. jejuni CIP 70.2 T to mucin. In conclusion, in vitro assays showed that Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, as viable or heat-killed forms, are adherent to different intestinal matrix models and are highly aggregative in vitro with pathogens, especially Campylobacter spp., the most commonly reported zoonotic agent in the European Union. This study supports the need for further in vivo investigations to demonstrate the potential food safety benefits of Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, live or heat-killed, in the global feed/food chain.

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Section: Discussionmentioning

confidence: 89%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

113

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“…Previous studies showed that bacteria from different origins were less likely to have direct physical binding as revealed by coaggregation assay [27]. Using BHI cultivated mice gut microbial community [20] we further tested the interbacterial binding between oral species and gut flora.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies employing the standard coaggregation assay have shown that bacteria from different microbial communities exhibit less interbacterial binding than species originating from the same community [27]. Therefore, we investigated the co-adherence profiles of F. nucleatum and S. mutans with a foreign flora using cultivated mice gut flora as a model community.…”

Section: Oral Bacterial Species Displayed Less Co-adherence With Micementioning

confidence: 99%

Analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction‐denaturing gradient gel electrophoresis profiling

Wang

et al. 2011

Int J Oral Sci

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Information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community. Current knowledge on this topic is heavily based on pariwise coaggregation of known, cultivable species. In this study, we employed a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to systematically analyze the co-adherence profiles of oral bacterial species, and achieved a more profound knowledge beyond pairwise coaggregation. Two oral bacterial species were selected to serve as "bait": Fusobacterium nucleatum (F. nucleatum) whose ability to adhere to a multitude of oral bacterial species has been extensively studied for pairwise interactions and Streptococcus mutans (S. mutans) whose interacting partners are largely unknown. To enable screening of interacting partner species within bacterial mixtures, cells of the "bait" oral bacterium were immobilized on nitrocellulose membranes which were washed and blocked to prevent unspecific binding. The "prey" bacterial mixtures (including known species or natural saliva samples) were added, unbound cells were washed off after the incubation period and the remaining cells were eluted using 0.2 mol· L -1 glycine. Genomic DNA was extracted, subjected to 16S rRNA PCR amplification and separation of the resulting PCR products by DGGE. Selected bands were recovered from the gel, sequenced and identified via Nucleotide BLAST searches against different databases. While few bacterial species bound to S. mutans, consistent with previous findings F. nucleatum adhered to a variety of bacterial species including uncultivable and uncharacterized ones. This new approach can more effectively analyze the co-adherence profiles of oral bacteria, and could facilitate the systematic study of interbacterial binding of oral microbial species.

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“…From this observation, the aggregation of L. brevis in response to xylan was not considered to involve a specific reaction mediated by proteins, such as lectin-like proteins, which were previously identified as factors for the aggregation and gastrointestinal tract adherence of Lactobacillus. 8,9,[12][13][14][15]23) However, another type of protein was considered to mediate xylan-induced aggregation based on the effects of pH and temperature on aggregation.…”

Section: )mentioning

confidence: 99%

“…8,9) LAB strains belonging to Lactobacillus brevis and Lactobacillus gasseri that recognize human blood type-A antigen through recognition of the sugar chain of the antigen were reported. 10,11) Mannose-specific adhesion to the porcine jejunal epithelium by Lactobacillus plantarum 12) and coaggregation between and among human intestinal and oral bacteria via lectin-carbohydrate interactions 13) have also been reported. Furthermore, the aggregation of LAB and yeast cells mediated by mannose and/or mannan in the yeast cell wall has been confirmed for L. plantarum 14,15) and Lactococcus lactis.…”

mentioning

confidence: 99%

Xylan-mediated aggregation of Lactobacillus brevis and its relationship with the surface properties and mucin-mediated aggregation of the bacteria

Saito

Nakamura

Kobayashi

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Some Lactobacillus brevis strains were found to aggregate upon the addition of xylan after screening for lactic acid bacteria that interact with plant materials. The S-layer proteins of cell surface varied among the strains. The strains that displayed xylan-mediated aggregation retained its ability even after the removal of S-layer proteins. L. brevis had negative zeta potentials. A correlation between the strength of aggregation and zeta potential was not observed. However, partial removal of S-layer proteins resulted in decreases in the electric potential and aggregation ability of some strains. Therefore, xylan-mediated aggregation of L. brevis was considered to be caused by an electrostatic effect between the cells and xylan. L. brevis also aggregated in the presence of mucin, and the strengths of aggregation among the strains were similar to that induced by xylan. Thus, xylan- and mucin-mediated L. brevis aggregation was supposed to be caused by a similar mechanism.

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Coaggregation between and among human intestinal and oral bacteria

Cited by 55 publications

References 39 publications

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction‐denaturing gradient gel electrophoresis profiling

Xylan-mediated aggregation of Lactobacillus brevis and its relationship with the surface properties and mucin-mediated aggregation of the bacteria

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