Coaggregation between Prevotella nigrescens and Prevotella intermedia with Actinomyces naeslundii strains

Cookson, Ann

doi:10.1016/0378-1097(95)00331-x

Cited by 7 publications

(6 citation statements)

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“…Prevotella intermedia and Prevotella nigrescens both form coaggregating pairs with A. naeslundii . In contrast to many other oral microbial pairs, the interaction between these microorganisms does not require calcium [236]. Table 9 lists the free energy of coaggregation, defined in analogy to and calculated from the measurement of contact angles on microbial lawns employing the Lifshitz‐van der Waals/acid‐base approach ().…”

Section: Mechanisms Of Microbial Coaggregationmentioning

confidence: 99%

Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for study

1999

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In this review, initial microbial adhesive interactions are divided into adhesion to substratum surfaces, coaggregation between microbial pairs and co-adhesion between sessile and planktonic microorganisms of different strains or species. The physico-chemical mechanisms underlying the adhesive interactions are described and a critical review is given of currently employed methods to study microbial adhesive interactions, with an emphasis on the use of the parallel plate flow chamber. Subsequently, for each of the three microbial adhesive interactions distinguished, the role of Lifshitz-van der Waals, acid-base and electrostatic interactions is described based on existing literature.

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Section: Mechanisms Of Microbial Coaggregationmentioning

confidence: 99%

Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for study

1999

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“…A surface thermodynamic analysis of bacterial coaggregation, based on interfacial free energies calculated by the acid‐base approach of Van Oss et al [51] could explain the non‐cation mediated coaggregation of Prevotella intermedia and Prevotella nigrescens with A. naeslundii [59]. However, Ca‐mediated coaggregation, such as occurs between S. oralis and A. naeslundii , could not be explained by surface thermodynamics [49].…”

Section: Mechanisms Of Microbial Adhesive Interactionsmentioning

confidence: 99%

Adhesive interactions between medically important yeasts and bacteria

et al. 1998

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Yeasts are being increasingly identified as important organisms in human infections. Adhesive interactions between yeasts and bacteria may contribute to yeast retention at body sites. Methods for studying adhesive interactions between bacterial strains are well known, and range from simple macroscopic methods to flow chamber systems with complex image analysis capabilities. The adhesive interactions between bacteria and yeasts have been studied employing several of the methods originally developed for studying adhesive interactions between bacteria. However, in many of the methods employed the larger size of the yeasts as compared with bacteria results in strong sedimentation of the yeasts, often invalidating the method adapted. In addition, most methods are semi-quantitative and do not properly control mass transport. Consequently, adhesive interaction mechanisms between yeasts and bacteria identified hitherto, including lectin binding and protein-protein interactions, must be regarded with caution. Extensive physico-chemical characteristics of yeast cell surfaces are not available and a physico-chemical mechanism has not yet been put forth. A new method for quantifying adhesive interactions between yeasts and bacteria is proposed, based on the use of a parallel plate flow chamber, in which the influence of adhering bacteria upon the kinetics of yeast adhesion and aggregation of the adhering yeasts is quantitatively evaluated, under carefully controlled mass transport.

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“…Lactose (0.06 M) and sucrose (0.06 M) were used to inhibit the coaggregation reactions as described previously. 46,47 Additionally, the inhibition of bacterial coaggregation was assessed after incubating bacterial strains in protease (from Streptomyces griseus, P-5130; Sigma-Aldrich Corp., Castle Hill, Australia). 42 The bacterial strains were incubated for 2 hours in lactose, sucrose, or pronase at ambient temperature and then the cell suspensions were washed three times with coaggregation buffer to remove unbound inhibitors.…”

Section: Inhibition Of Bacterial Coaggregationmentioning

confidence: 99%