Coagulase deficiency in clinical isolates of Staphylococcus aureus involves both transcriptional and post-transcriptional defects

Vandenesch, F.; Lebeau, Christine; Bes, Michèle; McDevitt, Damien; Greenland, Timothy; Novick, Richard P.; Etienne, Jérôme

doi:10.1099/00222615-40-5-344

Cited by 33 publications

(22 citation statements)

References 2 publications

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“…b Two strains giving a false-negative coagulase reaction, even though they possess the coagulase gene (19 Among the 99 S. aureus collection strains, two "typical" isolates from patients admitted to the same unit of an English hospital gave negative results by the Staphychrom II test. These two strains showed the same pulsed-field gel electrophoresis restriction pattern and belonged to the same epidemic clone.…”

Section: Discussionmentioning

confidence: 99%

“…Only two atypical S. aureus collection strains were falsely negative by the Staphychrom II test. These two strains possess the coagulase gene but do not produce coagulase (19).…”

Section: Discussionmentioning

confidence: 99%

“…They comprised six S. aureus strains and five S. epidermidis strains. Among the atypical S. aureus strains, one was negative for lactose, trehalose, and mannitol acidification; one was negative by latex agglutination with several reagents; two were negative by TCT (19); and two were negative by the catalase test (A. Le Coustumier, Y. Brun, M. Bes, A.…”

Section: Test Organisms From March 2002 To May 2002 a Total Of 293mentioning

confidence: 99%

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Specific Identification of Staphylococcus aureus by Staphychrom II, a Rapid Chromogenic Staphylocoagulase Test

et al. 2004

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We compared the performance of Staphychrom II (International Microbio, Signes, France), a rapid (2-h) chromogenic staphylocoagulase test that uses human prothrombin and protease inhibitors, with those of the reference tube coagulase test (TCT) and the latex agglutination test (LAT) Slidex Staph Plus for the rapid identification of S. aureus. Prospective evaluation with 293 fresh clinical isolates yielded sensitivities, specificities, and predictive and negative predictive values of 98.1, 100, 100, and 95.1%, respectively, for the Staphychrom II test; 98.6, 98.7, 99.6, and 96.3%, respectively, for LAT; and 97.6, 98.7, 99.5, and 93.9%, respectively, for TCT. The perfect specificity of the Staphychrom II test was confirmed by testing 193 collection strains selected because of their potential testing pitfalls. The Staphychrom II test was positive for 90% of the 215 S. aureus strains tested after only 1 h of incubation. The Staphychrom II test was as sensitive as the reference TCT and was 100% specific.Rapid identification of Staphylococcus aureus is a major priority for clinical microbiologists. The tube coagulase test (TCT) is the reference method for distinguishing S. aureus and rare coagulase-positive non-S. aureus staphylococci (e.g., S. intermedius) from coagulase-negative staphylococci. However, this test requires 18 to 24 h of incubation and is not perfectly reliable (1,13,16,18). Agglutination tests with either erythrocytes or latex particles coated with various ligands may lack sensitivity, notably, for methicillin-resistant S. aureus (MRSA), and may also lack specificity for non-S. aureus staphylococci, such as S. lugdunensis, S. schleiferi, and S. intermedius (3,8,14,15).In the present study we evaluated Staphychrom II (International Microbio, Signes, France), a rapid (2-h) chromogenic staphylocoagulase test which specifically detects S. aureus, and compared the results obtained with those obtained by a reference TCT (Becton Dickinson, Le Pont de Claix, France) and the latex agglutination test (LAT) Slidex Staph Plus (bioMérieux, Marcy l'Etoile, France). The tests were compared prospectively with 293 clinical isolates in two clinical laboratories and retrospectively with 193 typical and atypical collection strains chosen because of their potential testing pitfalls. MATERIALS AND METHODS Test organisms. From March 2002 to May 2002, a total of 293Staphylococcus isolates were prospectively tested at Bellevue Hospital, Saint Etienne, France (n ϭ 149) or Debrousse Hospital, Lyon, France (n ϭ 144). They comprised 215 S. aureus isolates (43 MRSA strains and 172 methicillin-susceptible strains) and 78 non-S. aureus staphylococci. The 78 non-S. aureus isolates comprised S. capitis (n ϭ 14), S. caprae (n ϭ 3), S. cohnii (n ϭ 1), S. epidermidis (n ϭ 49), S. haemolyticus (n ϭ 3), S. hominis (n ϭ 4), S. simulans (n ϭ 2), and S. warneri (n ϭ 2). The strains were isolated from the following clinical specimens: pus (n ϭ 85), respiratory tract (n ϭ 83), nose (n ϭ 32), blood (n ϭ 26), catheter (n ϭ 22), urine (n ϭ 13), sto...

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Section: Discussionmentioning

confidence: 99%

“…Only two atypical S. aureus collection strains were falsely negative by the Staphychrom II test. These two strains possess the coagulase gene but do not produce coagulase (19).…”

Section: Discussionmentioning

confidence: 99%

Section: Test Organisms From March 2002 To May 2002 a Total Of 293mentioning

confidence: 99%

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Specific Identification of Staphylococcus aureus by Staphychrom II, a Rapid Chromogenic Staphylocoagulase Test

et al. 2004

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“…Coagulase activity may therefore represent a possible means of detecting these bacteria directly in milk. According to Vandenesch et al (1994), an inoculum of at least 10 8 bacteria ('pure culture') per ml is required for a reliable positive result in the standard coagulase test and, as Staph. aureus does not grow in rabbit plasma, incubation times longer than 4 h do not compensate for smaller inocula.…”

Section: Discussionmentioning

confidence: 99%

Simple and direct detection of Staphylococcus aureus in milk by a tube coagulase test

Yazdankhah

Olsen²

1998

Lett Appl Microbiol

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N. 1998. A tube coagulase test (TCT) is described as a simple and non-expensive system for detection of Staphylococcus aureus directly in milk. The procedure is characterized by mixing milk samples with rabbit citrate plasma followed by incubation at 37°C for clot formation. The tube coagulase test demonstrated 91·5% accuracy, 88·5% sensitivity and 100% specificity for the direct recognition of Staph. aureus in milk samples from quarters with subclinical mastitis, when compared with plating of milk on blood agar. The TCT has the potential to detect other coagulase positive staphylococci in milk. It is concluded that TCT may be of use to veterinary practitioners with limited laboratory facilities, or to dairy farmers as a simple diagnostic test on site.

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“…Some reports indicate that in some strains the gene may be lost, while others suggest the occurrence of an allelic variant of coa, the gene fbpA, which encodes a fibrinogen-binding protein and occurs alternatively to coa at the same site in the replicon (11). About 60% of CNSA (coagulase-negative S. aureus) strains isolated in France were found to be resistant to methicillin (16). Atypical S. aureus strains occurring in other countries are also exclusively or primarily MRSA strains (8,12).…”

Section: Introductionmentioning

confidence: 99%

Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods

Marek

Pyzik

Stępień–Pyśniak

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to identify the affinity of 10 Staphylococcus strains isolated from table chicken eggs to specific species. Preliminary analysis performed by API ID32 Staph test identified these strains as S. aureus, but they exhibited a negative reaction in the tube coagulase test. Thus, the analysed strains were initially characterised as Staphylococcus aureus-like (SAL). Further characterisation was performed by genotypic methods, using restriction fragment length polymorphism (RFLP) of the coagulase gene (coa) and sequencing of the gene rpoB. An attempt was also made to identify the isolated Staphylococcus strains by MALDI-TOF mass spectrometry. The results indicated that none of the strains tested belonged to the species S. aureus. The rpoB sequences of five isolates showed the highest sequence similarity to S. haemolyticus, three isolates to S. chromogenes, and one isolate to S. epidermidis. One strain (SAL4) remained unidentified in this analysis. The results obtained using mass spectrometry were comparable to those based on gene sequence analysis. Strain SAL4, which could not be identified by sequencing, was identified by MALDI-TOF as Staphylococcus chromogenes.

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Coagulase deficiency in clinical isolates of Staphylococcus aureus involves both transcriptional and post-transcriptional defects

Abstract: Coagulase deficiency in clinical isolates of Staph y o co ccus a ure us i n vo I ves bo t h t r a n sc r i p t i o n a I a n d post-transcriptional defects

Cited by 33 publications

References 2 publications

Specific Identification of Staphylococcus aureus by Staphychrom II, a Rapid Chromogenic Staphylocoagulase Test

Specific Identification of Staphylococcus aureus by Staphychrom II, a Rapid Chromogenic Staphylocoagulase Test

Simple and direct detection of Staphylococcus aureus in milk by a tube coagulase test

Identification of strains with phenotypes similar to those of Staphylococcus aureus isolated from table chicken eggs using MALDI-TOF MS and genotyping methods

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