Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health

Hamza, Dalia; Dorgham, Sohad M.; Arafa, Amany A.

doi:10.4236/aid.2015.54025

Cited by 7 publications

(6 citation statements)

References 32 publications

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“…The isolate showed positive results in coa test tube and based on other identification test results phenotypically identified as S. aureus . This contradiction results between traditional and molecular method also reported in a former study [ 24 ] where 10 strains that classified as coa negative by coa test tube were found to be positive by a molecular method using PCR. The findings emphasize the use of molecular methods in the identification and detection of S. aureus [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 60%

Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction

et al. 2019

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Aim: This study was conducted to study the coagulase (coa) gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle from three different regions in East Java Province, Indonesia. Materials and Methods: A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of S. aureus. The presumptive isolates were confirmed by coa test. The confirmed S. aureus isolates were subjected to coa gene polymerase chain reaction. Results: Of 160 different samples, 20 (12.5%) isolates of S. aureus were confirmed by positive coa test. Of 20 S. aureus isolates, 19 (95%) isolates carried coa gene. Six different genotypes of coa gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One coa genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region. Conclusion: It can be concluded that coa gene is easily epidemiological tool for detection of variation strain from S. aureus.

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Section: Discussionmentioning

confidence: 60%

Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction

et al. 2019

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“…This emphasizes the use of molecular methods in the accurate detection of coa gene in S. aureus . A similar study carried out in 2015 found that 10 isolates, classified as coagulase-negative by tube coagulase test, were found to be positive with coa gene PCR [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…This finding is in agreement with a study carried out in 2007, which reported the presence of many variants of coagulase genotypes from regions of their study, but they indicated that only a few genotypes were predominant [ 19 ]. In a study carried out in 2015, all PCR products for coa gene were detected at 750 bp [ 18 ]. In our study, polymorphism of 4 coa gene products by RFLP using HaeIII and AluI restriction endonucleases was studied, and four RFLP patterns p1, p2, p3, and p4 were obtained.…”

Section: Discussionmentioning

confidence: 99%

Molecular typing of Staphylococcus aureus based on coagulase gene

Javid¹,

Taku²,

Bhat³

et al. 2018

Vet World

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AimThis study was conducted to study the coagulase gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle using restriction fragment length polymorphism (RFLP) and their sequence-based phylogenetic analysis.Materials and MethodsA total of 192 different samples from mastitic milk, nasal cavity, and pus from skin wounds of cattle from Military Dairy Farm, Jammu, India, were screened for the presence of S. aureus. The presumptive isolates were confirmed by nuc gene-based polymerase chain reaction (PCR). The confirmed S. aureus isolates were subjected to coagulase (coa) gene PCR. Different coa genotypes observed were subjected to RFLP using restriction enzymes Hae111 and Alu1, to obtain the different restriction patterns. One isolate from each restriction pattern was sequenced. These sequences were aligned for maximum homology using the Bioedit softwareandsimilarity in the sequences was inferred with the help of sequence identity matrix.ResultsOf 192 different samples,39 (20.31%) isolates of S. aureus were confirmed by targeting nuc gene using PCR. Of 39 S. aureus isolates, 25 (64.10%) isolates carried coa gene. Four different genotypes of coa gene, i.e., 514 bp, 595 bp, 757 bp, and 802 bp were obtained. Two coa genotypes, 595 bp (15 isolates) and 802 bp (4 isolates), were observed in mastitic milk. 514 bp (2 isolates) and 757 bp (4 isolates) coa genotypes were observed from nasal cavity and pus from skin wounds, respectively. On RFLP using both restriction enzymes, four different restriction patterns P1, P2, P3, and P4 were observed. On sequencing, four different sequences having unique restriction patterns were obtained. The most identical sequences with the value of 0.810 were found between isolate S. aureus 514 (nasal cavity) and S. aureus 595 (mastitic milk), and thus, they are most closely related. While as the most distant sequences with the value of 0.483 were found between S. aureus 514 and S. aureus 802 isolates.ConclusionThe study, being localized to only one farm, yielded different RFLP patterns as observed from different sampling sites, which indicates that different S. aureus coagulase typeshave a site-specific predilection. Two coa patterns were observed in mastitic milk indicating multiple origins of infection, with 595 bp coa genotype being predominant in mastitic milk. The coa genotypes and their restriction patterns observed in the present study are novel, not published earlier. 514 and 595 coa variants of S. aureus are genetically most related.

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“…Like spa, coa has a polymorphic repeat region that can be used for differentiating S. aureus isolates. The variable region of coa gene is comprised of 81 bp tandem short sequence repeats (SSRs) [21].…”

Section: Discussionmentioning

confidence: 99%

Relationship between Antibiotic Resistance Patterns and Coagulase in Clinical Isolates of Staphylococcus aureus in Nowshahr and Chalous

Sajadi

Kaboosi

Ghadikolii

2017

Zahedan J Res Med Sci

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Background: Staphylococcus aureus is one of the main agents of nosocomial infections. Identifying coagulase enzyme in staphylococcal infections is of great importance as it is one of the main virulence agents. The present study aims to accurately detect the presence of coagulase gene and its relationship with antibiotic resistance patterns in clinical isolates. Methods: In this experimental study, a total of 20 clinical samples of S. aureus and 10 clinical samples of coagulase-negative staphylococci were collected and identified from hospitals and laboratories in Nowshahr and Chalous. Antibiotic resistance patterns of isolates were examined based on CLSI and finally polymerase chain reaction was used in order to detect the presence of coagulase gene with specific primers. Treatments were examined in three intervals and the results were analyzed using one-way analysis of variance (ANOVA), univariate ANOVA, and t-test at the probability level of P < 0.05. Moreover, the comparison of means was presented using Duncan's multiple range tests (P < 0.05). All the statistical analyses were carried out using SPSS (V. 16). Results: Suggested that isolates showed phenotypic presence of coagulase enzyme and had this gene genotypically as well. Coagulase-positive samples demonstrated higher resistance to tetracycline 20%, oxacillin 55%, gentamicin 20%, and kanamycin 25%. Statistically, there was no significant difference among inhibition zone diameters of oxacillin, nalidixic acid, gentamicin, kanamycin, and penicillin. Conclusions: Coagulase gene causes antibiotic resistance and coagulase-negative staphylococci are spreading rapidly and their resistance is increasing.

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Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health

Cited by 7 publications

References 32 publications

Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction

Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction

Molecular typing of Staphylococcus aureus based on coagulase gene

Relationship between Antibiotic Resistance Patterns and Coagulase in Clinical Isolates of Staphylococcus aureus in Nowshahr and Chalous

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