Coagulase Negative Staphylococcus Species Isolated from Blood Culture: A Pathogen or a Contaminant?

Abstract

Introduction: Coagulase negative staphylococci (CoNS) are the most frequent blood culture isolates and an important cause of nosocomial blood stream infections especially in catheterised patients. CoNS are also the most common contaminants of blood cultures and are proven to be especially problematic. These uncertainties may result in over diagnosis and indirect overuse of antimicrobials especially vancomycin. Aim: To determine the clinical significance of CoNS isolated from blood culture of patients admitted in a tertiary care hospital. Materials and Methods: A prospective cross-sectional study was conducted in Bharati Hospital Research Center, Pune, Maharashtra, India, from August 2019 to July 2020 (One year). Patients whose paired/multiple blood culture samples showed pure growth of CoNS were included in the study. CoNS were identified by Vitek 2 system and antimicrobial susceptibility was reported. Clinical history of all patients was taken who showed pure growth of CoNS in either one or multiple sites of the automated blood culture bottles. Isolated CoNS were considered as pathogen only if clinical and laboratory parameters are fulfilled. Chi-square test was used to find out statistical significance of isolated pathogenic CoNS. Results: A total 147 CoNS isolated from blood cultures of suspected patients of sepsis were included in study. About 23 (15.6%) CoNS were isolated from both the sites. Remaining 124 (84.4%) CoNS were isolated from single site. CoNS were considered as pathogen in all 23 cases based on clinical and laboratory criteria. The most frequent isolated CoNS was S. haemolyticus 7 (30.4%), followed by S. epidermidis 5 (21.7%), S. hominis 3 (13.1%) , S. lugdunensis 3 (13.1%), S. scuiri 2 (8.7%), S. xylosus 1 (4.3%), S. caprae 1 (4.3%), S. capitis 1 (4.3%). Methicillin resistance was observed in 15 (65.2%) CoNS strains. Conclusion: Patients suffering from CoNS from their blood stream should be carefully evaluated clinically. Proper blood collection techniques need to be followed to avoid contamination of blood culture samples and to avoid diagnostic dilemma.

Prevalence, Antimicrobial Resistance, and Molecular Characteristics of Coagulase-Negative Staphylococci Isolated from Children’s Blood Cultures in Northeastern Iran Within 2013 - 2019

“…strains isolated from the blood samples of children admitted to Imam Reza Hospital in Bojnurd, Northeastern Iran. The CoNS ruled out contaminants and were selected as pathogens based on a laboratory-based algorithm and clinical examinations by related physicians (13). The clinical criteria included the presence of one or more of the following factors based on the patient's history:…”