Coagulation Factor V Leiden Mutation Was Detected in the Patients with Activated Protein C Resistance in Thailand

Effects on Hemostasis After Two-Year Use of Low Dose Combined Oral Contraceptives with Gestodene or Levonorgestrel

“…These changes may not occur in other non-Caucasian populations as evidence has shown that thrombosis may be less common in East Asian (8,9) and Singapore women (10). However, a recent report (11) of activated protein C resistance (APCR) found in 46.3% of patients with thrombosis in Thailand may change this myth of relative immunity from thromboembolism in Asian subjects. This present study was done to characterize the changes in clotting parameters in Singapore women on OCs to see if the changes were different from their Caucasian counterparts.…”

A Thai Patient with the Mutation of Arg306 of FV Gene Identical to the Hong Kong but not to the Cambridge Type

“…PCR for detection of the AGG-codon of Arg306 was performed on genomic DNA using the described primers (4) 5'-TGTCCTAACT-CAGCTGGGAT-3' and 5'-GTATGAACCCCAACAACTCA-3' and AmpliTaq Gold DNA polymerase (Perkin Elmer) with a program of [1] preheating at 94°C for 12 min [2] 50 cycles of amplification (94°C for 1 min, 58°C for 1 min, and 72°C for 1 min) [3] final incubation at 72°C for 20 min. PCR product was sequenced directly with an automatic sequencer (ABI Prism 301, Perkin Elmer).…”

“…The A allele has been found in 1-2% of subjects in the healthy Caucasian populations analysed to date (2). Its prevalence in other ethnic groups is poorly documented, but it has not so far been found among Black Africans (3), Amerindians (Brazil) (3,4) or Asians (Japanese) (3,5,6), while it is very infrequent in African Americans (7) and Asian Indians (8). The prevalence of this prothrombin allele has not yet been studied in North African populations.…”