Coat color determination by miR-137 mediated down-regulation of microphthalmia-associated transcription factor in a mouse model

Dong, Changsheng; Wang, Haidong; Li, Xue; Dong, Yanjun; Yang, Lei; Fan, Rong; Yu, Xiuju; Xue, Tian; Ma, Shen; Smith, George W.

doi:10.1261/rna.033977.112

Cited by 86 publications

(81 citation statements)

References 33 publications

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“…Our results show that UV treatment in fish leads to the activation of a-MSH and the MC1R gene. The result is similar to that previously reported in keratinocytes (Dong et al, 2012). By contrast, miR-429 knockdown has no effect on the level of a-MSH and MC1R expression with or without UV radiation.…”

Section: Altered Expression Of Pigmentation Genes In Mir-429 Silencinsupporting

confidence: 91%

“…Skin pigmentation in fish is a complex process that involves a series of cellular, genetic and physiological factors (Colihueque, 2010). The role of miRNAs in pigmentation has been reported in some species, including mouse, alpaca and Drosophila (Dong et al, 2012;Kennell et al, 2012;Zhu et al, 2010). In this study, we revealed that miR-429 is a potential regulator of fish pigmentation.…”

Section: Altered Expression Of Pigmentation Genes In Mir-429 Silencinmentioning

confidence: 62%

“…In a previous study, miR-203 was found to define a molecular boundary between proliferative basal progenitors and terminally differentiating suprabasal cells, ensuring proper identity of neighboring layers (Yi et al, 2008). The expression level of miR-137 can affect the body color pattern in mice (Dong et al, 2012). In Drosophila, the loss of miR-8 shows a substantially decreased pigmentation of the dorsal abdomen (Kennell et al, 2012).…”

Section: Introductionmentioning

confidence: 95%

“…Skin containing pheomelanins were cleared by centrifugation at 10,700 g for 10 minutes and analyzed for absorbance at 400 nm. A400/mg5spectrophotometric pheomelanin (Sp.PM) (Dong et al, 2012).…”

Section: Spectrophotometric Assay Of Melaninmentioning

confidence: 99%

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microRNA regulation of skin pigmentation in fish

Yan

Liu

Zhu

et al. 2013

Journal of Cell Science

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SummaryMicroRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in numerous biological processes. However, the role of miRNAs in skin color determination in fish has not been completely determined. Here, we identified that 13 miRNAs are differentially expressed between red and white skin. The analysis of miRNA spatial and temporal expression patterns suggests that miR-429 is a potential regulator of skin pigmentation. miR-429 silencing results in an obvious change in skin pigmentation. Bioinformatics analysis and a luciferase reporter assay show that miR-429 directly regulates expression of Foxd3 by targeting its 39-untranslated (39-UTR) region. miR-429 silencing leads to a substantial increase in the expression of Foxd3 in vivo, thereby repressing the transcription of MITF and its downstream genes, such as TYR, TYRP1 or TYRP2. These findings would provide a novel insight into the determination of skin color in fish.

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Section: Altered Expression Of Pigmentation Genes In Mir-429 Silencinsupporting

confidence: 91%

Section: Altered Expression Of Pigmentation Genes In Mir-429 Silencinmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 95%

Section: Spectrophotometric Assay Of Melaninmentioning

confidence: 99%

See 2 more Smart Citations

microRNA regulation of skin pigmentation in fish

Yan

Liu

Zhu

et al. 2013

Journal of Cell Science

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“…In recent years, the regulatory mechanisms of alpaca fiber growth and coat color have aroused scientific interest, due to the excellent quality of alpaca wool Fan et al, 2010;Zhu et al, 2010;Dong et al, 2012). It has been recently shown that microRNAs (miRNAs) play pivotal roles in skin morphogenesis and development, and are involved in a variety of skin diseases, including cancer (Sand et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5

et al. 2015

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ABSTRACT. MicroRNAs are very small endogenous RNA molecules that play a crucial role in an array of biological processes, including regulation of skin morphogenesis. The microRNA let-7b is thought to modulate animal hair growth, by binding target genes that encode growth factors. Fibroblast growth factor 5 (FGF5) has been previously reported to be involved in the initiation of the catagen phase of hair growth. In this study, we combined previous reports with bioinformatic analysis techniques to identify and validate FGF5 and, using lucerifase assay, confirmed targeted binding of let-7b to FGF5. To investigate the interaction between let-7b and FGF5, alpaca skin fibroblasts were transfected with let-7b over-expression vectors, and then mRNA and protein expression levels of FGF5 and the gene encoding its receptor, FGFR1, were evaluated. Levels of FGF5 mRNA and protein were remarkably lower in transfected groups, as compared to controls. In summary, this study confirmed that let-7b acts as a regulator of skin morphogenesis, by directly targeting FGF5 and down-regulating its 13754-13763 (2015) expression. It provides the evidence of hair growth regulated by miRNAs in animals and may have important applications in wool production.

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Overexpression of miR‐122 promotes apoptosis of dermal papilla cells by directly targeting IGF1R in androgenetic alopecia

Liu

et al. 2021

Cell Biology International

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Androgenetic alopecia (AGA) is the most common pattern of hair loss resulting from the effects of androgen on hair follicles. MicroRNAs (miRs) serve imperative roles in the regulation of many biological processes of hair follicles. However, the exact molecular mechanism of AGA remains to be elucidated. In the present study, we found miR-122, which is mainly recognized as a tumor suppressor, was highly overexpressed in the bulb of balding hair follicles in comparison with nonbalding ones in AGA. Moreover, miR-122 induces apoptosis of human dermal papilla cells (hDPCs) with miR-122 mimics in vitro, and the expression of insulin-like growth factor 1 receptor (IGF1R) in hDPCs was reduced following upregulation of miR-122.Mechanistically, dual-luciferase reporter assay confirmed that miR-122 directly targeted the 3′-untranslated region of IGF1R. These findings suggested that upregulation of miR-122 induces apoptosis, potentially via the repression of IGF1R in hDPCs of AGA, providing a novel insight into the potential pathological mechanism of miR-122 in AGA DPCs.

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Coat color determination by miR-137 mediated down-regulation of microphthalmia-associated transcription factor in a mouse model

Cited by 86 publications

References 33 publications

microRNA regulation of skin pigmentation in fish

microRNA regulation of skin pigmentation in fish

Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5

Overexpression of miR‐122 promotes apoptosis of dermal papilla cells by directly targeting IGF1R in androgenetic alopecia

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