Coating liposomes with protein decreases their capture by macrophages

Торчилин, В. П.; Berdichevsky, V. R.; Barsukov, A. A.; Смирнов, В. Н.

doi:10.1016/0014-5793(80)80789-7

Cited by 46 publications

(14 citation statements)

References 9 publications

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“…Opsonization of nano-sized carriers and their subsequent capture by macrophages starts with the recognition of the carrier by receptors or binding sites on the membrane of endocytic cells. If liposomes coated with a protein, such as albumin and globulins in our case, are simultaneously present in an environment which has excess of the same protein, the capture of liposomes can be decreased due to preliminary saturation of the binding sites on macrophages for those proteins (Torchilin et al., 1980). Thus, the longevity in the circulation can be increased.…”

Section: Discussionmentioning

confidence: 99%

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Surface-engineered polyethyleneimine-modified liposomes as novel carrier of siRNA and chemotherapeutics for combination treatment of drug-resistant cancers

et al. 2019

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Modification of nanoparticle surfaces with PEG has been widely considered the gold standard for many years. However, PEGylation presents controversial and serious challenges including lack of functionality, hindered cellular interaction, allergic reactions, and stimulation of IgM production after repetitive dosing that accelerates blood clearance of the nanoparticles. We report the development of novel liposomal formulations surface-modified with a low molecular weight, branched polyethyleneimine (bPEI)–lipid conjugate for use as an alternative to PEG. The formulations had very good stability characteristics in ion- and protein-rich mediums. Protein adsorption onto the liposomal surface did not interfere with the cellular interaction. bPEI-modified liposomes (PEIPOS) showed enhanced association with three different cell lines by up to 75 times compared to plain or PEGylated liposomes and were without carrier toxicity. They also penetrated the deeper layers of 3D spheroids. Encapsulating paclitaxel (PTX) into PEIPOS did not change its main mechanism of action. PEIPOS complexed and intracellularly delivered siRNAs and downregulated resistance-associated proteins. Finally, tumor growth inhibition was observed in a mouse ovarian xenograft tumor model, without signs of toxicity, in animals treated with the siRNA/PTX co-loaded formulation. These complex-in-nature but simple-in-design novel liposomal formulations constitute viable and promising alternatives with added functionality to their PEGylated counterparts.

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Section: Discussionmentioning

confidence: 99%

“…Torchilin et al. (1980) elegantly showed that the albumin- and ɣ-globulin-coated liposomes were captured 20% and 25% less by the macrophages, respectively, and that this phenomenon was free protein concentration-dependent. Moreover, Ehrenberg et al.…”

Section: Discussionmentioning

confidence: 99%

Surface-engineered polyethyleneimine-modified liposomes as novel carrier of siRNA and chemotherapeutics for combination treatment of drug-resistant cancers

et al. 2019

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“…It has been evidenced that biodistribution of targeting colloidal carriers is determined by their surface properties such as surface charge and surface hydrophobicity in addition to their size [1 -11]. In general, highly charged and hydrophobic particles tend to be more rapidly taken up by phagocytes especially those of the RES [4 -9], whereas those with hydrophilic surface exhibit reduced RES clearance and sustained circulation time in the vascular compartment [10][11][12][13][14][15][16][17][18], and thus possess capability of targeting drugs to diseased sites, e.g. tumors [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

Moselhy

Nicholov

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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The pH- and temperature-responsive poly(N-isopropylacrylamide-co-methacrylic acid) (PNIPAm/MAA) nanoparticles are of potential application in targeted drug delivery. Their responsive properties in the presence of human serum albumin were investigated using dynamic light scattering (DLS), protein assay, and electron spin resonance (ESR) spectroscopy. Their interaction with human monocytes and polymorphonuclear leukocytes (PMNLs) was studied using scanning electron microscopy (SEM) and oxygen consumption method. The nanoparticles exhibited a volume phase transition at 35-40 degrees C in Hanks balanced salt solution (HBSS) and in phosphate buffer solution (PBS) of pH 7.4. The diameter of the nanoparticles decreased slightly in the presence of HSA at 25 degrees C at neutral pH, whereas an increase in the diameter in pH 6 PBS at 40 degrees C was revealed. The amount of albumin adsorbed onto the nanoparticles decreased with increasing temperature. The ESR spectra of spin labeled HSA indicated a more restricted environment in the nanoparticles at elevated temperatures. The stimulation of PMNL oxygen consumption by PNIPAm based nanoparticles, an indication of phagocytosis of the particles, was not observed regardless whether the nanoparticles were incubated in plasma or serum. In contrast, the more hydrophobic polystyrene (PSt) particles induced a significant increase in the rate of oxygen consumption after the incubation. PNIPAm/MAA-grafted-PSt particles behaved similarly to the PNIPAm/MAA nanoparticles, suggesting that surface properties dictate the recognition of colloids by PMNLs.

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“…Many approaches to reducing RES uptake have been reported. These include the use of glycolipids, polysaccharides, proteins, and polymers to modify the properties of liposomes (Allen and Chonn 1987;Gabizon and Papahadjopoulos 1988;Litzinger et al 1994;Sunamoto and Iwamoto 1986;Torchilin et al 1980). Liposomes carrying a surface-attached hydrophilic group such as polyethylene glycol (PEG) elude the RES.…”

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confidence: 98%

Long circulating liposomes of 2′,3′-dideoxyinosine: Formulation and stability

1996

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2',3'-Dideoxyinosine (ddI), an anti-human immunodeficiency virus (HIV) agent, was encapsulated in liposomes. The influence of the phospholipidkholesterol ratio, concentration of phospholipid (PL), and chain length of PL on the encapsulation of ddI in multilamellar vesicles (MLVs), frozen and thawed multilamellar vesicles (FAT MLVs), and large unilamellar vesicles (LUVs) was studied. An optimum formulation was then selected to prepare long circulating liposomes. Stability studies at 4,25, and 37°C and under certain stress conditions were performed. Release characteristics in phosphate buffer (pH 7.4) at 37°C were studied. Results show an increase in encapsulation efficiency (EE) with increasing amounts of cholesterol, a decrease in EE and increase in encapsulation yield (EY) with increasing concentrations of PL, and an increase in EE with increases in PL chain length, in both MLVs and LUVs. Freezing and thawing of MLVs had no influence on EE at a PL concentration of 10 mg/mL but increased EE at higher concentrations of PL. Various stability tests showed the formulation to be stable to leakage of entrapped drug when stored at 4,25, and 37°C for 6 months, when subjected to mechanical stress, and on exposure to human serum. The release studies indicated that 70% of ddI was released over a period of 72 h.

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Coating liposomes with protein decreases their capture by macrophages

Cited by 46 publications

References 9 publications

Surface-engineered polyethyleneimine-modified liposomes as novel carrier of siRNA and chemotherapeutics for combination treatment of drug-resistant cancers

Surface-engineered polyethyleneimine-modified liposomes as novel carrier of siRNA and chemotherapeutics for combination treatment of drug-resistant cancers

In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

Long circulating liposomes of 2′,3′-dideoxyinosine: Formulation and stability

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