2018
DOI: 10.1016/j.jcis.2018.07.028
|View full text |Cite
|
Sign up to set email alerts
|

Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton

Abstract: We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells. Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at co… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
11
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
8
1

Relationship

1
8

Authors

Journals

citations
Cited by 11 publications
(11 citation statements)
references
References 52 publications
0
11
0
Order By: Relevance
“…Although the majority of research on PPOL has concentrated on cells of the monocyte/macrophage/foreign body giant cell/osteoclast lineage, other cells in the BMU, as well as fibroblasts, vascular progenitors and others play prominent roles in the development of PPOL. Previous research has documented adverse effects of different byproducts of wear on osteoblasts [15,84,106,[112][113][114], osteocytes [83,84,115], and mesenchymal stem cells and osteoblast progenitors [116][117][118]. In general, chronic inflammation associated with byproducts of wear suppresses bone formation by inhibiting the proliferation, differentiation, maturation and function of progenitor cells and their downstream lineage cells.…”
Section: The Implant-bone Interface: a Series Of Bone Multicellular Umentioning
confidence: 99%
“…Although the majority of research on PPOL has concentrated on cells of the monocyte/macrophage/foreign body giant cell/osteoclast lineage, other cells in the BMU, as well as fibroblasts, vascular progenitors and others play prominent roles in the development of PPOL. Previous research has documented adverse effects of different byproducts of wear on osteoblasts [15,84,106,[112][113][114], osteocytes [83,84,115], and mesenchymal stem cells and osteoblast progenitors [116][117][118]. In general, chronic inflammation associated with byproducts of wear suppresses bone formation by inhibiting the proliferation, differentiation, maturation and function of progenitor cells and their downstream lineage cells.…”
Section: The Implant-bone Interface: a Series Of Bone Multicellular Umentioning
confidence: 99%
“…The following settings were employed to excite the dyes and acquire the images: λ ex 540–545 nm and λ ex 340 nm; λ em 570–573 nm and λ em 488 nm for tetramethyl rhodamine B isothiocyanate-conjugated phalloidin and Hoechst, respectively. The obtained images were processed using ZEN imaging software (Zeiss) 29…”
Section: Methodsmentioning
confidence: 99%
“…This indicated that Co NPs were more cytotoxic than Ti NPs. 66 Moreover, decreased mineralization ability, elasticity, spring constant, and increased apoptosis seemed more pronounced by Co NPs than Ti NPs. Similar results have been observed using murine MC3T3-E1 osteoblasts: both Co elemental (30 nm) and Co (II, III) oxide (50 nm) NPs caused a decline in metabolic activity and osteoblast mineralization ability after a 21-day challenge.…”
Section: Metal Npsmentioning
confidence: 97%
“…Furthermore, increased elasticity and spring constant and decreased osteoblastic mineralization ability were observed. 66 Notably, Ti NPs induced a less pronounced effect than Co NPs, 66 possibly as a result of Ti NPs’ lower uptake rate and better cytocompatibility. The same group also compared Ti with Co NPs on MC3T3E1 cells in vitro.…”
Section: Metal Npsmentioning
confidence: 99%