Cochlear nucleus whole mount explants promote the differentiation of neuronal stem cells from the cochlear nucleus in co-culture experiments

Rak, Kristen; Völker, Johannes; Jürgens, Lukas; Völker, Christine; Frenz, Silke; Scherzad, Agmal; Schendzielorz, Philipp; Jablonka, Sibylle; Mlynski, Robert; Radeloff, Andreas; Hagen, Rudolf

doi:10.1016/j.brainres.2015.04.055

Cited by 9 publications

(9 citation statements)

References 52 publications

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“…The differentiation of NSCs is dependent on the microenvironment and the regulation of various genes ( 21 – 23 ). Previous studies have demonstrated that the development of NSCs is closely associated with the Wnt, BMP and sonic hedgehog signaling systems.…”

Section: Discussionmentioning

confidence: 99%

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage

Zhang

Zhu

Luo

et al. 2016

Molecular Medicine Reports

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The aim of the present study was to investigate the possible damage-repair mechanisms of neural stem cells (NSCs) following hypoxic-ischemic brain damage (HIBD). NSCs obtained from Sprague Dawley rats were treated with tissue homogenate from normal or HIBD tissue, and β-catenin expression was silenced using siRNA. The differentiation of NSCs was observed by immunofluorescence, and semiquantitative reverse transcription-polymerase chain reaction and western blot analysis were applied to detect the mRNA and protein expression levels of Ngn1 and BMP4 in the NSCs. Compared with control NSCs, culture with brain tissue homogenate significantly increased the differentiation of NSCs into neurons and oligodendrocytes (P<0.05), whereas differentiation into astrocytes was significantly reduced (P<0.05). Compared with negative control-transfected cells, knockdown of β-catenin expression significantly decreased the differentiation of NSCs into neurons and oligodendrocytes (P<0.01), whereas the percentage of NSCs differentiated into astrocytes was significantly increased (P<0.01). Compared with control NSCs, the mRNA and protein expression levels of Ngn1 were significantly increased (P<0.01) and BMP4 levels were significantly reduced (P<0.01) by exposure of the cells to brain tissue homogenate. Compared with the negative control plasmid-transfected NSCs, the levels of Ngn1 mRNA and protein were significantly reduced by β-catenin siRNA (P<0.01), whereas BMP4 levels were significantly increased (P<0.01). In summary, the damaged brain tissues in HIBD may promote NSCs to differentiate into neurons for self-repair processes. β-Catenin, BMP4 and Ngn1 may be important for the coordination of NSC proliferation and differentiation following HIBD.

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Section: Discussionmentioning

confidence: 99%

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage

Zhang

Zhu

Luo

et al. 2016

Molecular Medicine Reports

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“…To mimic these processes in vitro, the serum-free medium and B-27 supplement with retinoic acid were used for cell differentiation. The DIF medium described already has been used successfully in stem cell and differentiation protocols from the precedent studies on CN [14,16,17,38].…”

Section: Stem Cell Microenvironmentmentioning

confidence: 99%

“…It persists until adulthood, which was shown by an ongoing capacity of neurosphere formation, BrdU incorporation, and detection of NSCs by specific markers [16]. The NSCs of the CN were able to differentiate after transplantation into neural tissue in all cells of the neural lineage [17].…”

mentioning

confidence: 98%

Different Neurogenic Potential in the Subnuclei of the Postnatal Rat Cochlear Nucleus

Voelker¹,

Engert²,

Voelker³

et al. 2021

Stem Cells International

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In patients suffering from hearing loss, the reduced or absent neural input induces morphological changes in the cochlear nucleus (CN). Neural stem cells have recently been identified in this first auditory relay. Afferent nerve signals and their impact on the immanent neural stem and progenitor cells already impinge upon the survival of early postnatal cells within the CN. This auditory brainstem nucleus consists of three different subnuclei: the anteroventral cochlear nucleus (AVCN), the posteroventral cochlear nucleus (PVCN), and the dorsal cochlear nucleus (DCN). Since these subdivisions differ ontogenetically and physiologically, the question arose whether regional differences exist in the neurogenic niche. CN from postnatal day nine Sprague-Dawley rats were microscopically dissected into their subnuclei and cultivated in vitro as free-floating cell cultures and as whole-mount organ cultures. In addition to cell quantifications, immunocytological and immunohistological studies of the propagated cells and organ preparations were performed. The PVCN part showed the highest mitotic potential, while the AVCN and DCN had comparable activity. Specific stem cell markers and the ability to differentiate into cells of the neural lineage were detected in all three compartments. The present study shows that in all subnuclei of rat CN, there is a postnatal neural stem cell niche, which, however, differs significantly in its potential. The results can be explained by the origin from different regions in the rhombic lip, the species, and the various analysis techniques applied. In conclusion, the presented results provide further insight into the neurogenic potential of the CN, which may prove beneficial for the development of new regenerative strategies for hearing loss.

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“…11 Tissue engineering applied in curing peripheral nerve injury has become a promising approach in recent years. [12][13][14] In this work, we used modied gellan gum (TGG) loaded with laminin (LN) and nerve growth factor (NGF) to enhance the proliferation and differentiation of neuronal stem cells via a synergistic reinforcement mechanism. Gellan gum is a kind of anionic linear exopolysaccharide produced via aerobic fermentation by Sphingomonas.…”

Section: Introductionmentioning

confidence: 99%

Laminin-modified gellan gum hydrogels loaded with the nerve growth factor to enhance the proliferation and differentiation of neuronal stem cells

Huang

Zhong

et al. 2020

RSC Adv.

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Cochlear nucleus whole mount explants promote the differentiation of neuronal stem cells from the cochlear nucleus in co-culture experiments

Cited by 9 publications

References 52 publications

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage

Different Neurogenic Potential in the Subnuclei of the Postnatal Rat Cochlear Nucleus

Laminin-modified gellan gum hydrogels loaded with the nerve growth factor to enhance the proliferation and differentiation of neuronal stem cells

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