Cochliobolus Phylogenetics and the Origin of Known, Highly Virulent Pathogens, Inferred from ITS and Glyceraldehyde-3-Phosphate Dehydrogenase Gene Sequences

First Report of Leaf Spot of Datura metel Caused by Alternaria tenuissima in Korea

“…ITS1 and ITS4 primers (White et al, 1990) was used to amplify the internal transcribed spacer region (ITS) region containing ITS1-5.8S-ITS2 of nuclear ribosomal DNA (rDNA), and glycerol-3-phosphate dehydrogenase (G3PDH) (Berbee et al, 1999), the RNA polymerase II second largest subunit (RPB2) (Liu et al, 1999) two nuclear protein-coding genes were sequenced. PCR was performed in a total volume of 25 ml by using 0.5 ml of dNTP, 2.5 units of Taq DNA polymerase (0.5 ml of 5 U/ml enzyme; Bioneer, Daejeon, Korea), 2.0 ml of genomic DNA, 2.5 ml of 10× PCR reaction buffer and 5 pmol/l of each primer (0.5 ml each).…”

Porocercospora seminalis gen. et comb. nov., the causal organism of buffalograss false smut

“…In the phylogenetic study, 11 species of Curvularia, eight of Bipolaris, four of Exserohilum and one of Pyrenophora (designated outgroup) were included (TABLE I). The latter two genera were considered in the analysis because they are morphologically similar and phylogenetically close to members of the Bipolaris-Curvularia complex (Sivanesan 1987, Berbee et al 1999.…”

Feohifomicosis nasal por Curvularia spicifera en un paciente pediátrico con neutropenia y leucemia mieloide aguda

“…Al comparar los géneros Curvularia y Bipolaris se observa que los primeros tienden a tener conidias más cortas (en la mayoría de las especies menor a 100 μm) y son usualmente rectos o curvos; los curvos tienen células intermedias más grandes lo cual contribuye a su curvatura. Las conidias de las especies Bipolaris son usualmente más largas y separadas que las de Curvularia (usualmente mayores a 100 μm) y pueden ser rectas o levemente curvas 4,39,40 . Existen escasas comunicaciones de infecciones por Curvularia spp.…”