Cockroach-allergen study: Allergen patterns of three common cockroach species probed by allergic sera collected in two cities

Kang, Bann C.; Wilson, Marijo E.; Price, Kathleen H.; Kambara, Takahito

doi:10.1016/0091-6749(91)92152-q

Cited by 42 publications

(22 citation statements)

References 13 publications

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“…The most common and major domiciliary cockroaches are the American and German cockroaches both in Taiwan (11,12) and the United States (28,29). The existence of common IgE-binding components shared in whole-body extracts of the American and German cockroaches have been suggested (13,(15)(16)(17). Two important allergens of B. germanica have recently been cloned and sequenced.…”

Section: Discussionmentioning

confidence: 99%

“…A high prevalence of cockroach hypersensitivity in atopic (20 -55%) and asthmatic (49 -60%) populations has been documented (2,3,8,9,11). The American cockroach allergens with molecular weights ranging from 6000 to 120,000 have previously been identified by various standard immunochemical techniques (8,(12)(13)(14)(15)(16)(17)(18)(19)(20). Previous work in our group identified partially purified Cr-PI and Cr-PII allergenic fractions from crude American cockroach extract (CRa-A) 1 (12).…”

mentioning

confidence: 99%

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Sequencing Analysis of cDNA Clones Encoding the American Cockroach Cr-PI Allergens

Lee

Liao

et al. 1996

Journal of Biological Chemistry

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A previous article described the isolation of several gt22A cDNA clones expressing the American cockroach (Periplaneta americana) Cr-PI allergens recognized by both human atopic IgE antibodies and anti-Cr-PI monoclonal antibodies (Wu, C. H., Lee, M. F., and Liao, S. C. (1995) J. Allergy Clin. Immunol. 96, 352-359). This article presents the nucleotide and deduced amino acid sequences of two cDNA clones encoding major allergens of P. americana. Clones C12 and C20 encode proteins of 685 and 631 amino acids with two potential N-glycosylation sites each. The predicted molecular weights for C12 and C20 cloned proteins are 79,300 and 75,500 with isoelectric point values of 6.26 and 6.63, which are compatible with the determined sizes (M r 78,000 and 72,000) and isoelectric point value (6.2) of the Cr-PI allergens of P. americana. A high degree of identity (69.1%), including several overlapped predicted central antigenic determinant residues, was found between two allergens. The anti-fusion protein antibody-based enzyme-linked immunosorbent assay was able to detect crude American cockroach extract, Cr-PI, recombinant proteins, and commercial cockroach extracts, which provides further evidence that two allergens share common antigen determinants. Recombinant allergens of clones C12 and C20 both showed 47.4% skin reactivities on 19 cockroach-sensitive asthmatic patients. Unexpectedly, although no sequence similarity was found to other known allergens, two aromatic amino acid-rich allergens were found to have a striking sequence identity to insect storage proteins (20.1-33.9%), insect juvenile hormone-suppressible proteins (30.9 -36.4%), and arthropod hemocyanins (29.7-34.6%). Results suggested that two prominent allergens of P. americana are ancestrally related to these insect hemolymph proteins and represent a new group of proteins in the hemocyanin superfamily. These data will now facilitate epitope-mapping studies, and the recombinant allergens may be valuable for diagnostic and therapeutic purposes.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Sequencing Analysis of cDNA Clones Encoding the American Cockroach Cr-PI Allergens

Lee

Liao

et al. 1996

Journal of Biological Chemistry

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“…Cockroaches are particularly important in low-income housing (Ôinner cityÕ) where they can cause severe asthma (292). In certain hot and humid regions of the United States (506,507) or tropical areas such as South East Asia (508)(509)(510), allergies to cockroaches are as frequent or even more frequent than allergies to ragweed pollen or to HDMs. However, cockroaches are also prevalent in many European countries (511)(512)(513) and even in Nordic countries (514).…”

Section: Other Mitesmentioning

confidence: 99%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,867

1,761

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“…Cockroaches are a very important source of domestic allergens in the U.S.A. [111] but their impact on allergic diseases in the U.K. has not yet been studied.…”

Section: Allergens Of Other Animals In the Homementioning

confidence: 99%