Cocos nucifera L. endosperm promotes healing of excised wound in BALB/c mice

Abstract

Mature endosperm of Cocos nucifera L. (family Arecaceae) has been used in folkloric medicine as wound healing agent. However, valuable scientific evidence of its traditional use has not yet been verified. This study evaluates the wound healing activity of C. nucifera endosperm on the excised wound in BALB/c mice. Three concentrations (30%, 60% and 90%) of C. nucifera endosperm were prepared as ointment using petroleum jelly as base. The safety of the formulation was evaluated using dermal irritation test. The prepared concentrations, Solcoseryl® ointment (positive control) and petroleum jelly (negative control) were topically applied daily on the excised wound and observed for wound healing activity for 14 days, with histological evaluations at days 7 and 14. The 90% C. nucifera endosperm ointment showed no clinical signs of dermal irritation during the study duration, confirming the safety for topical usage of all the C. nucifera formulation. All groups demonstrated scab formation and wound contraction at days 7 and 9, respectively. Healing of the wounds, manifested by distinct wound contraction, was observed at day 14, with 60% C. nucifera endosperm exhibiting significant activity compared to the negative control (p=0.009) and untreated (p=0.046) groups. It also displayed the highest activity, with notable similarity in the activity of Solcoseryl®, and revealed the most organized epidermis comparable to normal skin. This study validates the folkloric use of C. nucifera endosperm in facilitating wound healing process, with 60% C. nucifera endosperm exhibiting the most desirable activity.

Safety Assessment of Cocos nucifera (Coconut)-Derived Ingredients as Used in Cosmetics

“…20,[34][35][36] Various parts of the coconut including coconut liquid endosperm, husk fiber, and coconut juice, have also been researched for use as alternative or therapeutic treatments (as herbal medicines or dietary supplements) for dermal wounds, health promotion, and cardiovascular protection. [37][38][39][40][41] The electrolyte composition of coconut water (e.g., potassium) can cause vasodilation in blood vessels; therefore, coconut water has been used as intravenous fluid for hydration and as a resuscitation fluid. 42 Coconut flower clusters (also called inflorescence) and coconut juice are reportedly used in traditional folk medicine in Southeast Asia to treat menstrual cycle disorders.…”

Studi Literatur Aktivitas Antiinflamasi sebagai Penyembuh Luka Tumbuhan Obat Terpilih Berdasarkan Ramuan Usada Tiwas Panggung

“…Menurut penelitian, Air kelapa mengandung karbohidrat, lemak dan protein. Daging kelapa mengandung Asam aurat dan kandungan pada minyak kelapa adalah asam lemak yang terdiri dari asam kaproat, oktanoat, siklopropanpentanoat, laurat, miristat, palmitat, oleat, dan stearate [18], [19]. E e e e e e e e e e e Minyak kelapa murni (Virgin Coconut Oil) adalah minyak kelapa yang dibuat dari bahan baku kelapa segar, diproses dengan tanpa pemanasan sama sekali dan tanpa bahan kimia.…”

“…Tindakan antiinflamasi asam laurat dapat dikaitkan dengan penghambatan NF-kappa B dan aktivasi kaskade pada protein kinase yang diaktifkan mitogen. Hal ini dikuatkan dengan penelitian in vivo pada hewan pengerat yang menunjukkan bahwa area luka yang diobati dengan salep endosperma Cocos nucifera sembuh lebih cepat karena terjadi penurunan waktu epitelisasi yang signifikan dengan dengan p<0.05 [19]. Nuclear factor kappa B (NF-κB) merupakan suatu molekul protein yang terletak dalam sitoplasma dan berada dalam bentuk inaktif serta terikat dalam sel.…”