CoCr‐enriched medium modulates integrin‐based downstream signaling and requires a set of inflammatory genes reprograming in vitro

Abstract

Significant health concerns have been raised by the high levels of Cr and Co ions into whole blood as resulted of corrosion process released from biomedical implants, but very little is known about their biological behavior in governing cell metabolism. Thus, we prompted to address this issue by exploring the effects of CoCr enriched medium on both fibroblast and preosteoblast (pre-Ob) cells. First, we showed there is a significant difference in Co and Cr releasing dependent on engineered surface, it being even more released in dual acid-etching treating surface (named w/DAE) than the machined surfaces (named wo/DAE). Thereafter, we showed CoCr affects pre-osteoblast and fibroblast metabolism by dynamically modulating integrin-based downstream signaling (FAK, Src, Rac1, and Cofilin). Specifically on this matter, we have shown there is dynamic β1-integrin gene activation up 24 h in both preosteoblast and fibroblast. Our analysis showed also that both pre-Ob and fibroblast are important resource of proinflammatory cytokines when responding to CoCr enriched medium. In addition, survival-related signaling pathway was also affected interfering on survival and proliferating signal, mainly affecting CDK2, mapk-Erk and mapk-p38 phosphorylations, while AKT/PKB-related gene remained active. In addition, during cell adhesion PP2A (an important Ser/Thr phosphatase) was inactive in both cell lineages and it seems be a CoCr's molecular fingerprint, regulating specific metabolic pathways involved with cytoskeleton rearrangement. Altogether, our results showed for the first time CoCr affects cellular performance in vitro by modulating integrin activation-based downstream signaling and requiring a reprograming of inflammatory genes activations in vitro. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 839-849, 2018.

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“…Thus, our data show there is a dynamic actin rearrangement as consequence of the signal transduction upon integrin activation. On this sense, our data support the cytoskeleton rearrangement by modulating cofilin phosphorylation at Ser03, and it might be guaranteed by the inactivation of PP2A, a Ser/Thr phosphatase able to dephosphorylate cofilin . In our experimental model design, PP2A was up‐phosphorylated at Y307 and this phosphorylation status is related to the inhibition of this enzyme .…”

“…In our experimental model design, PP2A was up‐phosphorylated at Y307 and this phosphorylation status is related to the inhibition of this enzyme . Previously, we have explored this balance of cofilin/PP2A interactions in response to others biomaterials and this seems to be a fingerprint during cell adaptation on the biomaterial's surfaces and related environment.…”

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