Codelivery of IL-7 Augments Multigenic HCV DNA Vaccine-induced Antibody as well as Broad T Cell Responses in Cynomolgus Monkeys

Park, Su–Hyung; Song, Mi-Young; Nam, Hyo Jung; Im, Se Jin; Sung, Young‐Chul

doi:10.4110/in.2010.10.6.198

Cited by 16 publications

(9 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…IL-7 is known to induce expansion of T cells under lymphopenic conditions (24). In our previous studies, we demonstrated that IL-7 can be used as a vaccine adjuvant to augment antigen-specific T cell response (25,26) and to enhance antibody responses through the induction of follicular helper T cells (27). Here, we demonstrate that a single intranasal pretreatment with Fc-fused IL-7 (IL-7-mFc), but not a native form of IL-7, completely protects mice from IAV-induced mortality for an extended period of time, even without preexisting IAV-specific immunity.…”

mentioning

confidence: 99%

Intranasal Introduction of Fc-Fused Interleukin-7 Provides Long-Lasting Prophylaxis against Lethal Influenza Virus Infection

Kang

Choi

et al. 2016

J Virol

View full text Add to dashboard Cite

Influenza IMPORTANCEThe major consequence of a highly pathogenic IAV infection is severe pulmonary inflammation, which can result in organ failure and death at worst. Although vaccines for seasonal IAVs are effective, frequent variation of surface viral proteins hampers development of protective immunity. In this study, we demonstrated that intranasal IL-7-mFc pretreatment protected immunologically naive mice from lethal IAV infections. Intranasal pretreatment with IL-7-mFc induced an infiltration of T cells in the lung, which reside as effector/memory T cells with lung-retentive markers. Those IL-7-primed pulmonary T cells contributed to development of protective immunity upon IAV infection, reducing pulmonary immunopathology while increasing IAV-specific cytotoxic T lymphocytes. Since a single treatment with IL-7-mFc was effective in the protection against multiple strains of IAV for an extended period of time, our findings suggest a possibility that IL-7-mFc treatment, as a potential prophylaxis, can be developed for controlling highly pathogenic IAV infections.

show abstract

mentioning

confidence: 99%

Intranasal Introduction of Fc-Fused Interleukin-7 Provides Long-Lasting Prophylaxis against Lethal Influenza Virus Infection

Kang

Choi

et al. 2016

J Virol

View full text Add to dashboard Cite

show abstract

“…In the present study, we tested IL-7 and IL-33 as molecular adjuvants in HZ DNA vaccination. Co-administration of plasmids encoding IL-7 or IL-33 have been reported to enhance DNA vaccine-induced T-cell responses ( 27 28 30 31 ). Mice were immunized with pVAX1-gE combined with pVAX1-mIL-7 or pVAX1-mIL-33, and gE-specific T-cell responses evaluated by intracellular cytokine staining.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have shown that co-delivery of plasmids encoding various cytokines substantially enhances DNA vaccine-induced immune responses in diverse settings ( 20 38 39 ). In particular, plasmids encoding IL-7 or IL-33 have been shown to significantly enhance DNA vaccine-induced T-cell responses ( 27 28 30 31 ). In the current study, we evaluated the adjuvant effects of the combination of IL-7 and IL-33 for the first time and found that combined administration of IL-7 and IL-33 plasmids strongly enhanced HZ DNA vaccine-induced T-cell responses ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Previous reports demonstrated that in vivo administration of IL-7 results in the increase of T-cell numbers as well as antigen-specific, functional T-cell responses ( 24 25 26 ). Furthermore, there are evidences that co-delivery of IL-7 as a vaccine adjuvant can enhance DNA vaccine-induced T-cell immune responses ( 27 28 ). IL-33 is a member of the IL-1 cytokine family and acts as an endogenous ‘danger signal’ that triggers inflammation and promotes cell-mediated immune responses ( 29 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Herpes Zoster DNA Vaccines with IL-7 and IL-33 Molecular Adjuvants Elicit Protective T Cell Immunity

et al. 2018

Self Cite

View full text Add to dashboard Cite

Herpes zoster (HZ), or shingles, is caused by the reactivation of latent varicella-zoster virus (VZV) from the sensory ganglia when VZV-specific T-cell immunity is decreased because of aging or immunosuppression. In the present study, we developed HZ DNA vaccine candidates encoding VZV proteins and cytokine adjuvants, such as IL-7 and IL-33. We immunized C57BL/6 mice with DNA plasmids encoding VZV glycoprotein E (gE), immediate early (IE) 63, or IE62 proteins and found that robust VZV protein-specific T-cell responses were elicited by HZ DNA vaccination. Co-administration of DNA plasmids encoding IL-7 or IL-33 in HZ DNA vaccination significantly enhanced the magnitude of VZV protein-specific T-cell responses. Protective immunity elicited by HZ DNA vaccination was proven by challenge experiments with a surrogate virus, vaccinia virus expressing gE (VV-gE). A single dose of HZ DNA vaccine strongly boosted gE-specific T-cell responses in mice with a history of previous infection by VV-gE. Thus, HZ DNA vaccines with IL-7 and IL-33 adjuvants strongly elicit protective immunity.

show abstract

“…Imperative role by IL-7 in T-cell memory prompted the molecule to be tested for its adjuvanted effect in its DNA and protein forms. It showed increased T-cell response against hepatitis C virus infection [ 22 ] and avian influenza [ 23 ]. IL-7tv, a modified IL-7 protein, which has a lower binding capacity than IL-7 with IL-7R successfully promoted CD4 and CD8 populations and reduced the IL-7 receptor down-regulation due to supra physiologic doses of IL-7 [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Expression of biologically active bovine interleukin 7 and evaluating the activity in vitro

Lijo¹,

Vijay²,

Dechamma³

et al. 2016

Vet World

View full text Add to dashboard Cite

Aim:Interleukin 7 (IL-7) is a ϒc family cytokine involved in the homeostatic proliferation and maintenance of immune cells. In the present study, we report the expression of bovine IL-7 (bIL-7) in Escherichia coli and evaluated for its biological activity.Materials and Methods:The sequence coding for bIL-7 (mature protein) was amplified from primary bovine kidney cell culture and cloned into pET28-a vector and expressed in E. coli (BL 21 DE3). The expressed protein was purified by nickel-nitrilotriacetatechromatography, and the reactivity of the protein was confirmed by western blotting using monoclonal antibodies raised against human IL-7. The biological activity of expressed bIL-7 was evaluated by analyzing its effect on the expression of anuclear factor for activated T-cells c1 (NFATc1), B-cell lymphoma 2 (Bcl2), suppressor of cytokine signaling 3 (SOCS3) molecules in bovine peripheral blood mononuclear cells (PBMCs) by quantitative polymerase chain reaction. Ability of the expressed protein was also analyzed by its effect on phosphorylating signal transducer and activator 3 (STAT3) molecule by immunostaining in human embryonic kidney cells 293 (HEK293) cells.Results:The bIL-7 was able to induce the expression of Bcl2 and NFATc1expression in bovine PBMCs by 7 and 5-folds, respectively, whereas a 2-fold decrease was observed in the case of SOCS3 expression. Immunostaining studies in HEK293 cells using antihuman phospho-STAT3 showed activation and nuclear translocation of STAT3 molecule on bIL-7 treatment.Conclusion:bIL-7 gene was successfully amplified, cloned, and expressed in a prokaryotic expression system. The biological activity study showed that the E. coli expressed bIL-7 protein is biologically active. Considering the role of IL-7 in T-cell homeostasis and memory cell generation, this molecule can be used for enhancing the vaccine response and that has to be proved by further experiments.

show abstract

Codelivery of IL-7 Augments Multigenic HCV DNA Vaccine-induced Antibody as well as Broad T Cell Responses in Cynomolgus Monkeys

Cited by 16 publications

References 33 publications

Intranasal Introduction of Fc-Fused Interleukin-7 Provides Long-Lasting Prophylaxis against Lethal Influenza Virus Infection

Intranasal Introduction of Fc-Fused Interleukin-7 Provides Long-Lasting Prophylaxis against Lethal Influenza Virus Infection

Herpes Zoster DNA Vaccines with IL-7 and IL-33 Molecular Adjuvants Elicit Protective T Cell Immunity

Expression of biologically active bovine interleukin 7 and evaluating the activity in vitro

Contact Info

Product

Resources

About