Coding and interaction of sex pheromone and plant volatile signals in the antennal lobe of the codling moth <i>Cydia pomonella</i>

Trona, Federica; Anfora, G.; Bengtsson, Marie; Witzgall, Peter; Ignell, Rickard

doi:10.1242/jeb.047365

Cited by 70 publications

(93 citation statements)

References 70 publications

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“…This result emphasizes the biological significance of pear ester and shows that the PR clade contains co-evolving receptors for sex pheromones and for host odorants. This corroborates the modulation of male sexual behavior by host plant odorants in codling moth (Trona et al, 2010(Trona et al, , 2013, and adds to our understanding of the evolution of sexual communication and olfaction-driven speciation in insect herbivores.…”

Section: Introductionsupporting

confidence: 76%

“…Intracellular recordings of axons of OSNs projecting to the antennal lobe (AL), the olfactory center of the insect brain, and functional imaging of AL glomeruli, receiving input from OSNs expressing the same ORs, support our finding that pear ester activates a dedicated olfactory channel and that interaction of pear ester with other compounds, including the sex pheromone codlemone, takes place in the AL, and not at the periphery (Figure 3; Trona et al, 2010Trona et al, , 2013.…”

Section: Cpomor3 Is Tuned To the Plant Volatile Pear Estersupporting

confidence: 60%

“…Indeed, closely related ORs with high sequence similarity are often expressed in OSNs that project to neighboring glomeruli in the AL (Couto et al, 2005;Masse et al, 2009;Ramdya and Benton, 2010;Cande et al, 2013). Accordingly, the architecture of the codling moth AL lends support to the hypothesis that the OR genes for pear ester and codlemone, the codling moth sex pheromone, are closely related-the glomeruli dedicated to pear ester and codlemone are adjacent glomeruli in the codling moth AL, where stimulation with a blend of codlemone and pear ester produces a very strong synergistic effect (Trona et al, 2010(Trona et al, , 2013. Although the PR for codlemone has not yet been found, we can reasonably assume that it belongs to the PR clade, which contains the putative pheromone receptors CpomOR1, and CpomOR4 through 6 (Figure 1; Bengtsson et al, 2012).…”

Section: Interaction Between Pear Ester and Codling Moth Pheromonementioning

confidence: 79%

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A predicted sex pheromone receptor of codling moth Cydia pomonella detects the plant volatile pear ester

et al. 2014

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Plant volatiles mediate host discrimination and host finding in phytophagous insects. Understanding how insects recognize these signals is a current challenge in chemical ecology research. Pear ester, ethyl (E,Z )-2,4-decadienoate, is a powerful, bisexual attractant of codling moth Cydia pomonella (Lepidoptera, Tortricidae) and strongly synergizes the male response to female-produced sex pheromone. We show here that the codling moth odorant receptor (OR) CpomOR3 is dedicated to detecting this plant volatile. Heterologous expression of CpomOR3 in Drosophila T1 trichoid and ab3A basiconic sensilla, followed by a screening with codling moth pheromone compounds and known plant volatile attractants, confirms that CpomOR3 binds to pear ester. Although CpomOR3 does not respond to any of the pheromone components tested, a phylogenetic analysis of lepidopteran chemosensory receptor genes reveals a close relationship of CpomOR3 with pheromone receptors (PRs) in moths. This corroborates the interaction of ecological and social chemosensory cues during premating communication. The finding that a plant volatile compound, pear ester, is a specific ligand for a PR-like lepidopteran receptor adds to our understanding of insect-plant interactions and emphasizes the interaction of natural and sexual selection during the phylogenetic divergence of insect herbivores.

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Section: Introductionsupporting

confidence: 76%

Section: Cpomor3 Is Tuned To the Plant Volatile Pear Estersupporting

confidence: 60%

Section: Interaction Between Pear Ester and Codling Moth Pheromonementioning

confidence: 79%

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A predicted sex pheromone receptor of codling moth Cydia pomonella detects the plant volatile pear ester

et al. 2014

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“…Excitatory responses of MGC PNs to plant odours have also been reported for S. littoralis (Anton and Hansson, 1995), and inhibitory responses of MGC PNs to plant odours were described in M. sexta (Reisenman et al, 2008). Similar evidence has been found in the tortricid moths Cydia pomonella and Cydia molesta, where responses to plant odours were found in MGC neurons and pheromone responses in PNs within other glomeruli (Trona et al, 2010;Varela et al, 2011b). Such results are in agreement with data from vertebrates, where both 'common' odours and pheromones can activate the main olfactory bulb and the accessory olfactory bulb (Xu et al, 2005), and thus overlapping processing of both odour types occurs in both compartments of the primary olfactory centre.…”

Section: Mgc Neurons Are Sensitive To a Behaviourally Relevant Plant supporting

confidence: 67%

Plant odour stimuli reshape pheromonal representation in neurons of the antennal lobe macroglomerular complex of a male moth

Chaffiol¹,

Kropf²,

Barrozo³

et al. 2012

Journal of Experimental Biology

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SUMMARYMale moths are confronted with complex odour mixtures in a natural environment when flying towards a female-emitted sex pheromone source. Whereas synergistic effects of sex pheromones and plant odours have been observed at the behavioural level, most investigations at the peripheral level have shown an inhibition of pheromone responses by plant volatiles, suggesting a potential role of the central nervous system in reshaping the peripheral information. We thus investigated the interactions between sex pheromone and a behaviourally active plant volatile, heptanal, and their effects on responses of neurons in the pheromone-processing centre of the antennal lobe, the macroglomerular complex, in the moth Agrotis ipsilon. Our results show that most of these pheromone-sensitive neurons responded to the plant odour. Most neurons responded to the pheromone with a multiphasic pattern and were anatomically identified as projection neurons. They responded either with excitation or pure inhibition to heptanal, and the response to the mixture pheromone + heptanal was generally weaker than to the pheromone alone, showing a suppressive effect of heptanal. However, these neurons responded with a better resolution to pulsed stimuli. The other neurons with either purely excitatory or inhibitory responses to all three stimuli did not exhibit significant differences in responses between stimuli. Although the suppression of the pheromone responses in AL neurons by the plant odour is counterintuitive at first glance, the observed better resolution of pulsed stimuli is probably more important than high sensitivity to the localization of a calling female. Supplementary material available online at

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“…The insecticidal properties of some allyl esters have been assessed previously in order to give an added value to the surplus of glycerol [21,23,24]. As several volatile compounds from codling and grapevine moth host-plants are used by adults for host and mate localization [25][26][27][28], an effect in moth behavior due to allyl esters used as fruit aromas [29] was suspected. Moreover, some volatile compounds described as moth attractants [30][31][32] are chemically related to allyl cinnamate.…”

Section: Introductionmentioning

confidence: 99%

Antennal and Behavioral Response of Cydia pomonella and Lobesia botrana Moths to Allyl Cinnamate

Balcells¹

2014

Adv Crop Sci Tech

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This paper describes for the first time the capacity of allyl cinnamate to elicit antennal response and to modify behavior from C. pomonella and L. botrana adults. Improved understanding of the role of this chemical compound may ultimately be incorporated into Tortricidae integrated pest management programmes (IPM). Material and Methods InsectsThe experiments were conducted with a C. pomonella laboratory strain originated from a population collected in an unsprayed apple orchard in Lleida (north-east Spain), and with a L. botrana laboratory strain established in our laboratory from a mass-reared strain from INRA Bordeaux (France). Both strains were reared at the Crop Protection Laboratory of the UdL-IRTA Centre for Research and Development (Lleida, Spain) at 23 ± 2°C, with a photoperiod of 16:8 (L:D) and on agar-based semi-synthetic diet [33].Cydia pomonella individuals were sexed as last instar larvae, whereas L. botrana individuals were sexed as pupae. The sexed individuals were kept separately by sex until adult emergence in the same conditions as AbstractElectroantenographical (EAG) response to allyl cinnamate were assessed on virgin and mated Cydia pomonella and Lobesia botrana adults to determine whether this compound could be used within integrated management programs (IMP). Adult behavioral reaction was later assessed in a wind tunnel, with and without the main compound of the corresponding female sex pheromone.Allyl cinnamate elicited antennae responses of C. pomonella and L. botrana, both males and females. Allyl cinnamate EAG response was as high as pheromone response, and it was not reduced after mating.In wind tunnel assays, allyl ester itself was not attractive to C. pomonella males, but its presence did not interfere with the pheromonal action when the number of contacts was compared. For females, a higher proportion of codling moths moved towards the source when allyl cinnamate was in the wind-tunnel plume. No differences were recorded depending on the mating status of codling moth adults. The same trend was observed in L. botrana males and females.Results suggest that allyl cinnamate acts as a female behavioral modifier, but more assays are required to determine its role in insect communication in field conditions before inclusion in integrated pest management.

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Coding and interaction of sex pheromone and plant volatile signals in the antennal lobe of the codling moth Cydia pomonella

Abstract: SUMMARY

Cited by 70 publications

References 70 publications

A predicted sex pheromone receptor of codling moth Cydia pomonella detects the plant volatile pear ester

A predicted sex pheromone receptor of codling moth Cydia pomonella detects the plant volatile pear ester

Plant odour stimuli reshape pheromonal representation in neurons of the antennal lobe macroglomerular complex of a male moth

Antennal and Behavioral Response of Cydia pomonella and Lobesia botrana Moths to Allyl Cinnamate

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