Coding-Complete Genome Sequence and Phylogenetic Relatedness of a SARS-CoV-2 Strain Detected in March 2020 in Cameroon

Njouom, Richard; Sadeuh‐Mba, Serge Alain; Tchatchueng, Jules; Diagne, Moussa Moïse; Dia, Ndongo; Tagnouokam, Paul Alain Ngoupo; Boum, Yap; Hamadou, Achta; Esso, Linda; Faye, Ousmane; Tejiokem, Mathurin Cyrille; Okomo, Marie Claire; Etoundi, Alain; Carniel, Élisabeth; Eyangoh, Sara

doi:10.1128/mra.00093-21

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…Finally, the antigens in the ELISA kits used were designed to target the original SARS-CoV-2 lineage that emerged in China. Several variants of the virus, such as the Omicron or delta lineages, have been reported in Cameroon during pandemic waves [65][66][67], and thus, it is likely that a few infection cases were missed as these antigens may not optimally detect antibodies against SARS-CoV-2 variants and that the seroprevalence is underestimated.…”

Section: Discussionmentioning

confidence: 99%

Serological surveillance reveals a high exposure to SARS-CoV-2 and altered immune response among COVID-19 unvaccinated Cameroonian individuals

Moguem Soubgui,

Ndeme Mboussi,

Kojom Foko

et al. 2024

PLOS Glob Public Health

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Surveillance of COVID-19/SARS-CoV-2 dynamics is crucial to understanding natural history and providing insights into the population’s exposure risk and specific susceptibilities. This study investigated the seroprevalence of SARS-CoV-2 antibodies, its predictors, and immunological status among unvaccinated patients in Cameroon. A multicentre cross-sectional study was conducted between January and September 2022 in the town of Douala. Patients were consecutively recruited, and data of interest were collected using a questionnaire. Blood samples were collected to determine Immunoglobin titres (IgM and IgG), interferon gamma (IFN- γ) and interleukin-6 (IL-6) by ELISA, and CD4+ cells by flow cytometry. A total of 342 patients aged 41.5 ± 13.9 years were included. Most participants (75.8%) were asymptomatic. The overall crude prevalence of IgM and IgG was 49.1% and 88.9%, respectively. After adjustment, the seroprevalence values were 51% for IgM and 93% for IgM. Ageusia and anosmia have displayed the highest positive predictive values (90.9% and 82.4%) and specificity (98.9% and 98.3%). The predictors of IgM seropositivity were being diabetic (aOR = 0.23, p = 0.01), frequently seeking healthcare (aOR = 1.97, p = 0.03), and diagnosed with ageusia (aOR = 20.63, p = 0.005), whereas those of IgG seropositivity included health facility (aOR = 0.15, p = 0.01), age of 40–50 years (aOR = 8.78, p = 0.01), married (aOR = 0.21, p = 0.02), fever (aOR = 0.08, p = 0.01), and ageusia (aOR = 0.08, p = 0.01). CD4+, IFN-γ, and IL-6 were impaired in seropositive individuals, with a confounding role of socio-demographic factors or comorbidities. Although the WHO declared the end of COVID-19 as a public health emergency, the findings of this study indicate the need for continuous surveillance to adequately control the disease in Cameroon.

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Section: Discussionmentioning

confidence: 99%

Serological surveillance reveals a high exposure to SARS-CoV-2 and altered immune response among COVID-19 unvaccinated Cameroonian individuals

Moguem Soubgui,

Ndeme Mboussi,

Kojom Foko

et al. 2024

PLOS Glob Public Health

View full text Add to dashboard Cite

show abstract

“…Second, SARS-CoV-2 variants circulating in the country is also a plausible explanation ( Raman et al, 2021 ). Some studies reported the circulation of different SARS-CoV-2 variants in Cameroon ( Chouikha et al, 2022 ; Njouom et al, 2020 ). However, large epidemiological data on the extent of SARS-CoV-2 variants are still lacking in the country.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological situation of SARS-CoV-2 infection in Douala, the most populated and highly heterogeneous town of Cameroon: a post-vaccination update

et al. 2023

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“…Finally, the antigens in the ELISA kits used were designed to target the original SARS-CoV-2 lineage that emerged in China. Several variants of the virus, such as the Omicron or delta lineages, have been reported in Cameroon during pandemic waves [60][61][62], and thus, it is likely that a few infection cases were missed as these antigens may not optimally detect antibodies against SARS-CoV-2 variants and that the seroprevalence is underestimated.…”

Section: Discussionmentioning

confidence: 99%

Serological surveillance reveals a high exposure to SARS-CoV-2 and altered immune response among COVID-19 unvaccinated Cameroonian individuals

Moguem Soubgui,

Ndeme Mboussi,

Kojom Foko

et al. 2023

Preprint

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Background Surveillance of COVID-19/SARS-CoV-2 dynamics is crucial to understanding natural history and providing insights into the population’s exposure risk and specific susceptibilities. This study investigated the seroprevalence of SARS-CoV-2 antibodies, its predictors, and immunological status among unvaccinated patients in Cameroon. Materials and Methods A multicentre cross-sectional study was conducted between January and September 2022 in the town of Douala. Patients were consecutively recruited, and data of interest were collected using a questionnaire. Blood samples were collected to determine Immunoglobin titres (IgM and IgG) by ALFA, CD4+ cells by flow cytometry, and interferon gamma (IFN- γ) and interleukin-6 (IL-6) by ELISA. Results A total of 342 patients aged 41.5 ± 13.9 years were included. Most participants (75.8%) were asymptomatic. The overall prevalence of IgM and IgG was 49.1% and 88.9%, respectively. Ageusia and anosmia have displayed the highest positive predictive values (90.9% and 82.4%) and specificity (98.9% and 98.3%). The predictors of IgM seropositivity were being aged 60 – 70 years (aOR = 0.54, p = 0.02) and ageusia (aOR = 9.31, p = 0.01), whereas those of IgG seropositivity included health facility (aOR = 0.23, p = 0.02) and ageusia (aOR = 0.21, p = 0.04). CD4+, IFN-γ, and IL-6 were impaired in seropositive individuals, with a confounding role of socio-demographic factors or comorbidities. Conclusion Although the WHO declared the end of COVID-19 as a public health emergency, the findings of this study indicate the need for continuous surveillance to adequately control the disease in Cameroon.

show abstract

Coding-Complete Genome Sequence and Phylogenetic Relatedness of a SARS-CoV-2 Strain Detected in March 2020 in Cameroon

Cited by 4 publications

References 8 publications

Serological surveillance reveals a high exposure to SARS-CoV-2 and altered immune response among COVID-19 unvaccinated Cameroonian individuals

Serological surveillance reveals a high exposure to SARS-CoV-2 and altered immune response among COVID-19 unvaccinated Cameroonian individuals

Epidemiological situation of SARS-CoV-2 infection in Douala, the most populated and highly heterogeneous town of Cameroon: a post-vaccination update

Serological surveillance reveals a high exposure to SARS-CoV-2 and altered immune response among COVID-19 unvaccinated Cameroonian individuals

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