Codon Usage

Redwan, Raimi Mohamed; Parthasarathy, Suhanya; Hari, Riitta

doi:10.1016/b978-0-12-809633-8.20440-8

Cited by 1 publication

(1 citation statement)

References 52 publications

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“…The first group contained codons ending with T (GGT, GCT, TCT) while the second group contained codons ending with G or C (GTC, ATC, CTC, TTC, TAC, TGC, CCC, ACC, AAC, CAG, CGC, CAC, AAG, GAC, GAG). This analysis showed that 15 out of 18 amino acids (two amino acids Met and Trp only have one possible codon) favour either G or C at the third base in a codon, whereas only 3 favour either A or T and when there is a C in the second base, and G is avoided in the last codon base as CG combination may lead to methylation (Zhou et al , 2016; Redwan et al , 2019).…”

Section: Resultsmentioning

confidence: 99%

Translational control of fungal gene expression during the wheat-Fusarium graminearum interaction

Kage

Gardiner

Stiller

et al. 2021

Preprint

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To date, translational regulation of key genes controlling infection-related processes in fungal pathogens during their interactions with plants has not been studied. Here, we employed ribosome profiling (ribo-seq) to study translational responses and how such responses are coordinated with transcriptional changes in the fungal pathogen Fusarium graminearum (Fg), which causes Fusarium head blight (FHB), a destructive disease of cereal crops worldwide. Transcription and translation were not always coordinated with approximately 22% of Fg genes showing a discordant relationship during wheat infection. Nitrite reductase, which we show here as an important component of fungal virulence, is only regulated at the translational level in Fg. In addition, more than 1000 new open reading frames (ORFs), many of which are short and highly conserved, were identified in the Fg genome. Like in higher eukaryotes, translation is controlled by upstream ORFs (uORFs) in Fg during infection. Similarly, miRNAs control both transcription and translation in Fg during wheat infection. However, Fgdicer2-dependent miRNAs do not have a significant effect on transcriptional gene expression at the global outset. The ribo-seq study undertaken here for the first time in any fungal pathogen discovered novel insights about the biology of an important plant pathogen.

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Section: Resultsmentioning

confidence: 99%