Coenzyme Q10 production in recombinant Escherichia coli strains engineered with a heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathway

Zahiri, Hossein Shahbani; Yoon, Sang Hwal; Keasling, Jay D.; Lee, Si Hyoung; Kim, Seon Won; Yoon, Sung Chul; Shin, Yong Chul

doi:10.1016/j.ymben.2006.05.002

Cited by 74 publications

(45 citation statements)

References 40 publications

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“…Open and striped bars represent the results obtained at 24 and 48 h of culture, respectively. amorphadiene , carotenoids (Yoon et al, 2007(Yoon et al, , 2009 and Coenzyme Q 10 (Zahiri et al, 2006) by increasing the supply of IPP and DMAPP. Likewise, in this study we report FOH production from E. coli harboring the MVA pathway.…”

Section: Discussionmentioning

confidence: 99%

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

Wang

Yoon

Shah

et al. 2010

Biotech & Bioengineering

104

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Farnesol (FOH) production has been carried out in metabolically engineered Escherichia coli. FOH is formed through the depyrophosphorylation of farnesyl pyrophosphate (FPP), which is synthesized from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) by FPP synthase. In order to increase FPP synthesis, E. coli was metabolically engineered to overexpress ispA and to utilize the foreign mevalonate (MVA) pathway for the efficient synthesis of IPP and DMAPP. Two-phase culture using a decane overlay of the culture broth was applied to reduce volatile loss of FOH produced during culture and to extract FOH from the culture broth. A FOH production of 135.5 mg/L was obtained from the recombinant E. coli harboring the pTispA and pSNA plasmids for ispA overexpression and MVA pathway utilization, respectively. It is interesting to observe that a large amount of FOH could be produced from E. coli without FOH synthase by the augmentation of FPP synthesis. Introduction of the exogenous MVA pathway enabled the dramatic production of FOH by E. coli while no detectable FOH production was observed in the endogenous MEP pathway-only control.

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Section: Discussionmentioning

confidence: 99%

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

Wang

Yoon

Shah

et al. 2010

Biotech & Bioengineering

104

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“…The length of the prenyl chain can be manipulated by expressing heterologous prenyltransferases with different chain length specificities in E. coli (4,36,52,53). However, additional modifications of the isoprenoid chain, such as chain desaturations, require the recruitment of enzyme activities from other biosynthetic pathways.…”

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confidence: 99%

Biosynthesis of Ubiquinone Compounds with Conjugated Prenyl Side Chains

Lee

Salomon

Mijts

et al. 2008

Appl Environ Microbiol

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Enzymatic steps from two different biosynthetic pathways were combined in Escherichia coli, directing the synthesis of a new class of biomolecules-ubiquinones with prenyl side chains containing conjugated double bonds. This was achieved by the activity of a C 30 carotenoid desaturase, CrtN, from Staphylococcus aureus, which exhibited an inherent flexibility in substrate recognition compared to other carotenoid desaturases. By utilizing the known plasticity of E. coli's native ubiquinone biosynthesis pathway and the unusual activity of CrtN, modified ubiquinone structures with prenyl side chains containing conjugated double bonds were generated. The side chains of the new structures were confirmed to have different degrees of desaturation by mass spectrometry and nuclear magnetic resonance analysis. In vivo 14 C labeling and in vitro activity studies showed that CrtN desaturates octaprenyl diphosphates but not the ubiquinone compounds directly. Antioxidant properties of conjugated side chain ubiquinones were analyzed in an in vitro ␤-carotene-linoleate model system and were found to be higher than the corresponding unmodified ubiquinones. These results demonstrate that by combining pathway steps from different branches of biosynthetic networks, classes of compounds not observed in nature can be synthesized and structural motifs that are functionally important can be combined or enhanced.

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“…4) Recently, many researchers have tried to increase CoQ 10 production by overexpressing CoQ 10 biosynthetic enzymes of the methylerythritol phosphate (MEP) pathway and the mevalonate pathway. [5][6][7][8][9] Since these pathways are directly linked to CoQ 10 synthesis, direct enhancement of biosynthetic pathways can increase CoQ 10 production.…”

Section: Improvement Of Coenzyme Q 10 Production By Increasing the Namentioning

confidence: 99%

“…7,10,11) The discovery of the eubacterial MEP pathway has provided a means of engineering of the isoprenoid flux to improve CoQs productivity. Some groups have reported that production of CoQ 8 and of lycopene in E. coli was enhanced by overexpression of 1-deoxy-D-xylulose 5-phosphate synthase (Dxs) 12) or 1-deoxy-D-xylulose 5-phosphate (DXP) reductoisomerase, the enzymes catalyzing the first and second steps of the MEP pathway respectively.…”

Section: Improvement Of Coenzyme Q 10 Production By Increasing the Namentioning

confidence: 99%

Improvement of Coenzyme Q₁₀Production by Increasing the NADH/NAD⁺Ratio inAgrobacterium tumefaciens

Koo

Gong²,

Kim³

et al. 2010

Bioscience, Biotechnology, and Biochemistry

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Coenzyme Q10 production in recombinant Escherichia coli strains engineered with a heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathway

Cited by 74 publications

References 40 publications

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

Biosynthesis of Ubiquinone Compounds with Conjugated Prenyl Side Chains

Improvement of Coenzyme Q₁₀Production by Increasing the NADH/NAD⁺Ratio inAgrobacterium tumefaciens

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Coenzyme Q10 production in recombinant Escherichia coli strains engineered with a heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathway

Cited by 74 publications

References 40 publications

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

Farnesol production from Escherichia coli by harnessing the exogenous mevalonate pathway

Biosynthesis of Ubiquinone Compounds with Conjugated Prenyl Side Chains

Improvement of Coenzyme Q10Production by Increasing the NADH/NAD+Ratio inAgrobacterium tumefaciens

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Improvement of Coenzyme Q₁₀Production by Increasing the NADH/NAD⁺Ratio inAgrobacterium tumefaciens