Coenzyme Q10 protect against ischemia/reperfusion induced biochemical and functional changes in rabbit urinary bladder

Juan, Yung‐Shun; Hydery, Tasmina; Mannikarottu, Anita; Kogan, Barry A.; Schuler, Catherine; Leggett, Robert E.; Lin, Wei‐Yu; Huang, Chun‐Hsiung; Levin, Robert M.

doi:10.1007/s11010-007-9696-y

Cited by 33 publications

(22 citation statements)

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“…Three longitudinal strips measuring 0.5 ϫ 2.0 cm 2 were obtained from the bladder dome to the trigone area including both the muscle and mucosal layers. Contractile responses were measured as previously described (12,30). Briefly, each whole layer bladder strip was mounted in a separate 15-ml bath containing Tyrode solution (124.9 mM NaCl, 2.6 mM KCl, 23.8 mM NaHCO 3, 0.5 mM MgCl2, 0.4 mM NaH2PO4, 1.8 mM CaCl2, and 5.5 mM dextrose), which was equilibrated with a mixture of 95% O2 and 5% CO2.…”

Section: Methodsmentioning

confidence: 99%

Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum

Liu

Chuang

Long

et al. 2015

American Journal of Physiology-Renal Physiology

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Ketamine abusers develop severe lower urinary tract symptoms. The major aims of the present study were to elucidate ketamine-induced ulcerative cystitis and bladder apoptosis in association with oxidative stress mediated by mitochondria and the endoplasmic reticulum (ER). Sprague-Dawley rats were distributed into three different groups, which received normal saline or ketamine for a period of 14 or 28 days, respectively. Double-labeled immunofluorescence experiments were performed to investigate tight junction proteins for urothelial barrier functions. A TUNEL assay was performed to evaluate the distribution of apoptotic cells. Western blot analysis was carried out to examine the expressions of urothelial tight junction proteins, ER stress markers, and apoptosis-associated proteins. Antioxidant enzymes, including SOD and catalase, were investigated by real-time PCR and immunofluorescence experiments. Ketamine-treated rats were found to display bladder hyperactivity. This bladder dysfunction was accompanied by disruptions of epithelial cadherin- and tight junction-associated proteins as well as increases in the expressions of apoptosis-associated proteins, which displayed features of mitochondria-dependent apoptotic signals and ER stress markers. Meanwhile, expressions of mitochondria respiratory subunit enzymes were significantly increased in ketamine-treated bladders. Conversely, mRNA expressions of the antioxidant enzymes Mn-SOD (SOD2), Cu/Zn-SOD (SOD1), and catalase were decreased after 28 days of ketamine treatment. These results demonstrate that ketamine enhanced the generation of oxidative stress mediated by mitochondria- and ER-dependent pathways and consequently contributed to bladder apoptosis and urothelial lining defects. Such oxidative stress-enhanced bladder cell apoptosis and urothelial barrier defects are potential factors that may play a crucial role in bladder overactivity and ulceration.

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Section: Methodsmentioning

confidence: 99%

Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum

Liu

Chuang

Long

et al. 2015

American Journal of Physiology-Renal Physiology

Self Cite

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“…Partial outlet obstruction and in vivo models of ischemia have marked deleterious effects on superoxide dismutase and catalase, and as stated above have also been demonstrated to produce significant oxidative damage (10,(12)(13)(14). A variety of antioxidants and natural products that show significant antioxidant properties have been shown to protect the rabbit urinary bladder from contractile, cellular, and subcellular damage and dysfunction mediated by both partial outlet obstruction, and bilateral ischemia / reperfusion (15)(16)(17)(18)(19)(20). One of the most potent of these agents has been shown to be a grape suspension made from whole grapes (13,21,22).…”

Section: Introductionmentioning

confidence: 92%

Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

et al. 2010

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Purpose: The etiology of obstructive bladder dysfunction includes free radical damage to mitochondria. Feeding rabbits a standardized grape suspension protects the ability of the bladder to contract and empty in part by preventing mitochondrial damage, thus maintaining smooth muscle and mucosal metabolism. The objective of the current study is to determine the direct effect of this grape suspension on the response of mitochondria to the oxidative effects of hydrogen peroxide. Materials and Methods: Six male rabbits were anesthetized with sodium pentobarbital and the bladders excised. Four full thickness strips were obtained for contractile studies and the balance separated into smooth muscle and mucosa compartments by blunt dissection. The effect of hydrogen peroxide on the contractile response to field stimulation was quantitated. Each tissue was homogenized and the effects of increasing concentrations of hydrogen peroxide in the presence and absence of grape suspension on citrate synthase activity was determined. Results: Citrate synthase activity was significantly higher in the mucosa than in the muscle. The grape suspension had no effect on control citrate synthase activity. However, the grape suspension provided significant protection of both smooth muscle and mucosal citrate synthase activity. Conclusions: These studies support the conclusion that the grape suspension provides direct protection of mitochondrial function.

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“…Consumption of small amounts of dark chocolate also appears to confer cardioprotective effects, which may be mediated by epicatechin-mediated activation of d-opioid receptors [381]. Coenzyme Q-10 (CoQ-10), the vitamin-like substance that serves as an electron carrier between complexes II and III in the mitochondrial electron transport chain, has been found to confer protection in several models of IR when supplemented in the diet [219,314]; these protective effects were associated with less injury, increased endogenous antioxidant capacity, and lower oxidative stress. Coenzyme Q-10 (CoQ-10), the vitamin-like substance that serves as an electron carrier between complexes II and III in the mitochondrial electron transport chain, has been found to confer protection in several models of IR when supplemented in the diet [219,314]; these protective effects were associated with less injury, increased endogenous antioxidant capacity, and lower oxidative stress.…”

Section: Mediterranean Diet and Other Nutritional Manipulationsmentioning

confidence: 99%

Cell Survival Programs and Ischemia /Reperfusion: Hormesis, Preconditioning, and Cardioprotection

Krenz¹,

Baines²,

Kalogeris³

et al. 2013

Colloquium Series on Integrated Systems Physiology: From Molecule to Function

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ii This e-book is an original work commissioned for the Colloquium Digital Library of Life Sciences, a curated collection of time-saving pedagogical resources for researchers and students who want to quickly get up to speed in a new area of life science/biomedical research. Each e-book available in Colloquium is an in-depth overview of a fast-moving or fundamental area of research, authored by a prominent contributor to the field. We call these resources 'Lectures' because authors are asked to provide an authoritative, state-of-the-art overview of their area of expertise, in a manner that is accessible to a broad, diverse audience of scientists (similar to a plenary or keynote lecture at a symposium/meeting/colloquium). Readers are invited to keep current with advances in various disciplines, gain insight into fields other than their own, and refresh their understanding of core concepts in cell & molecular biology. Physiology is a scientific discipline devoted to understanding the functions of the body. It addresses function at multiple levels, including molecular, cellular, organ, and system. An appreciation of the processes that occur at each level is necessary to understand function in health and the dysfunction associated with disease. Homeostasis and integration are fundamental principles of physiology that account for the relative constancy of organ processes and bodily function even in the face of substantial environmental changes. This constancy results from integrative, cooperative interactions of chemical and electrical signaling processes within and between cells, organs and systems. This eBook series on the broad field of physiology covers the major organ systems from an integrative perspective that addresses the molecular and cellular processes that contribute to homeostasis. Material on pathophysiology is also included throughout the eBooks. The state-of the-art treatises were produced by leading experts in the field of physiology. Each eBook includes stand-alone information and is intended to be of value to students, scientists, and clinicians in the biomedical sciences. Since physiological concepts are an ever-changing work-in-progress, each contributor will have the opportunity to make periodic updates of the covered material.Published titles (for future titles please see the website, www.morganclaypool.com/page/lifesci) ABSTRACTThe major purpose of this book is to review the evidence supporting the concept that intrinsic cell survival programs can be activated by a variety of mildly noxious stimuli or pharmacologic agents to confer protection against the deleterious effects of ischemia/reperfusion (I/R). We begin with a discussion of the concept of hormesis (a term used most extensively in the toxicologic literature which refers to biphasic cellular responses that depend on concentration or intensity of a stimulus), review the seminal studies that led to the discovery of the cardioprotective effects of ischemic preconditioning, and outline its therapeutic potential (Chapter 1). This is followed ...

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Coenzyme Q10 protect against ischemia/reperfusion induced biochemical and functional changes in rabbit urinary bladder

Abstract: CoQ10 supplementation provides bladder protection against I/R injury. This protection effect improves mitochondrial function during I/R by repleting mitochondrial CoQ10 stores and potentiating their antioxidant properties.

Cited by 33 publications

References 32 publications

Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum

Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum

Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

Cell Survival Programs and Ischemia /Reperfusion: Hormesis, Preconditioning, and Cardioprotection

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