Coexistence of mupirocin and antiseptic resistance in methicillin-resistant Staphylococcus aureus isolates from Korea

Lee, Hyukmin; Lim, Hwan-Sub; Bae, Il Kown; Yong, Dongeun; Jeong, Seok Hoon; Lee, Kyungwon; Chong, Yunsop

doi:10.1016/j.diagmicrobio.2012.11.025

Cited by 32 publications

(16 citation statements)

References 31 publications

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“…Different methods have been proposed to detect reduced susceptibility to CHX [5,6,12]. One is based on the broth microdilution method [12], and although there is no clear definition of biocide resistance, in staphylococci CHX resistance is often defined as an MIC 4 mg/L [2].…”

Section: Discussionmentioning

confidence: 99%

“…PCR was also performed on all HLMR and LLMR isolates to detect the plasmid-associated ileS2 gene (mupA) [13]. The presence of qac (A/B and C) genes was determined by PCR using previously published primers [6].…”

Section: Detection Of Meca Mupa and Qac Genesmentioning

confidence: 99%

“…These genes are mainly found on plasmids and are associated with resistance to other biocides [4]. Some studies define CHX resistance on the basis of MRSA isolates possessing qac genes [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Both mupA and mupB are ileS genes imported from other species. A possible association between the presence of qac genes and resistance to MUP has been suggested [6,11].…”

Section: Introductionmentioning

confidence: 99%

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Chlorhexidine and mupirocin susceptibilities in methicillin-resistant Staphylococcus aureus isolates from bacteraemia and nasal colonisation

Muñoz‐Gallego

Infiesta

Viedma

et al. 2016

Journal of Global Antimicrobial Resistance

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Section: Discussionmentioning

confidence: 99%

Section: Detection Of Meca Mupa and Qac Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Both mupA and mupB are ileS genes imported from other species. A possible association between the presence of qac genes and resistance to MUP has been suggested [6,11].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Chlorhexidine and mupirocin susceptibilities in methicillin-resistant Staphylococcus aureus isolates from bacteraemia and nasal colonisation

Muñoz‐Gallego

Infiesta

Viedma

et al. 2016

Journal of Global Antimicrobial Resistance

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“…Such a cost-effective, rapid method of screening will allow early identification and control of dissemination in the event of prolific resistance. While several recently published multiplex assays are capable of detecting methicillin (mecA), mupirocin (mupA, mupB), and antiseptic (qacA, qacB, smr) resistance genes, none detect all six simultaneously while also individually identifying S. aureus and CoNS in a singletube reaction (21)(22)(23)(24). We applied our multiplex assay to test a total of 378 randomly selected clinical staphylococcal isolates and found 100% accuracy in the species differentiation of S. aureus as well as in the identification of methicillin and mupirocin resistance, as identified by the presence of nuc, mecA, and mupA and mupB, respectively.…”

Section: Discussionmentioning

confidence: 99%

Novel Multiplex PCR Assay for Detection of Chlorhexidine-Quaternary Ammonium, Mupirocin, and Methicillin Resistance Genes, with Simultaneous Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci

et al. 2017

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Methicillin-resistant Staphylococcus aureus (MRSA) is a clinically significant pathogen that is resistant to a wide variety of antibiotics and responsible for a large number of nosocomial infections worldwide. The Agency for Healthcare Research and Quality and the Centers for Disease Control and Prevention recently recommended the adoption of universal mupirocin-chlorhexidine decolonization of all admitted intensive care unit patients rather than MRSA screening with targeted treatments, which raises a serious concern about the selection of resistance to mupirocin and chlorhexidine in strains of staphylococci. Thus, a simple, rapid, and reliable approach is paramount in monitoring the prevalence of resistance to these agents. We developed a simple multiplex PCR assay capable of screening Staphylococcus isolates for the presence of antiseptic resistance genes for chlorhexidine and quaternary ammonium compounds, as well as mupirocin and methicillin resistance genes, while simultaneously discriminating S. aureus from coagulase-negative staphylococci (CoNS). The assay incorporates 7 PCR targets, including the Staphylococcus 16S rRNA gene (specifically detecting Staphylococcus spp.), nuc (distinguishing S. aureus from CoNS), mecA (distinguishing MRSA from methicillin-susceptible S. aureus), mupA and mupB (identifying high-level mupirocin resistance), and qac and smr (identifying chlorhexidine and quaternary ammonium resistance). Our assay demonstrated 100% sensitivity, specificity, and accuracy in a total of 23 variant antiseptic-and/or antibioticresistant control strains. Further validation of our assay using 378 randomly selected and previously well-characterized local clinical isolates confirmed its feasibility and practicality. This may prove to be a useful tool for multidrug-resistant Staphylococcus monitoring in clinical laboratories, particularly in the wake of increased chlorhexidine and mupirocin treatments.KEYWORDS chlorhexidine, coagulase-negative staphylococci, MRSA, methicillin, multiplex PCR, mupirocin, quaternary ammonium, resistance, antiseptic M ethicillin-resistant Staphylococcus aureus (MRSA) is a clinically significant pathogen that is resistant to a wide variety of antibiotics and responsible for a large number of nosocomial infections worldwide. Colonization with MRSA increases the risk of adverse health outcomes. Of those colonized, 10 to 30% will subsequently develop MRSA infections (1, 2) while also acting as reservoirs for transmission to other patients

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Benzalkonium Chloride

Kampf

2024

Antiseptic Stewardship

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Coexistence of mupirocin and antiseptic resistance in methicillin-resistant Staphylococcus aureus isolates from Korea

Cited by 32 publications

References 31 publications

Chlorhexidine and mupirocin susceptibilities in methicillin-resistant Staphylococcus aureus isolates from bacteraemia and nasal colonisation

Chlorhexidine and mupirocin susceptibilities in methicillin-resistant Staphylococcus aureus isolates from bacteraemia and nasal colonisation

Novel Multiplex PCR Assay for Detection of Chlorhexidine-Quaternary Ammonium, Mupirocin, and Methicillin Resistance Genes, with Simultaneous Discrimination of Staphylococcus aureus from Coagulase-Negative Staphylococci

Benzalkonium Chloride

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