Cognition-enhancing and neuroprotective activities of the standardized extract of Betula platyphylla bark and its major diarylheptanoids

Lee, Ki Yong; Jeong, Eun Ju; Huh, Jungmoo; Cho, Namki; Kim, Tae‐Bum; Jeon, Byung Ju; Kim, Seung Hyun; Kim, Hong Pyo; Sung, Sang Hyun

doi:10.1016/j.phymed.2012.09.012

Cited by 33 publications

(24 citation statements)

References 21 publications

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“…CREB activation leading to BDNF expression has been highlighted by previous reports on synaptic plasticity and cognitive function (Lee et al, 2012;Scott Bitner, 2012). Shorter durations of ischemia have been used in the experiments that aimed to test memory function .…”

Section: Discussionmentioning

confidence: 83%

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Beneficial Effects ofPolygonum multiflorumon Hippocampal Neuronal Cells and Mouse Focal Cerebral Ischemia

Ahn

Kim

et al. 2015

Am. J. Chin. Med.

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Beneficial effects of the water extract of Polygonum multiflorum (WEPM) and their mechanisms were investigated in HT22 hippocampal cells and hippocampus of middle cerebral artery occlusion (MCAO) mice. In HT22 cells against glutamate-induced oxidative stress, pretreatment with WEPM resulted in significantly reduced apoptotic neuronal death. Pretreatment with WEPM resulted in the suppression of ROS accumulation in connection with cellular Ca (2+) level after exposure to glutamate. Treatment with glutamate alone led to suppressed protein level of mature brain-derived neurotrophic factor (BDNF) and phosphorylated cAMP response element binding protein (CREB); however, pretreatment with either WEPM or anti-oxidant N-acetyl-ʟ-cysteine (NAC) resulted in the significant enhancement of levels of these proteins. In addition, levels of mature BDNF expression and CREB phosphorylation were increased by combined treatment with WEPM, NAC, and intracellular Ca (2+) inhibitor BAPTA compared to other treatment groups. In MCAO mice, we confirmed the critical role of mature BDNF expression and CREB phosphorylation by WEPM in the neurons of the hippocampus. Our results suggest that WEPM mainly exerted beneficial effects on hippocampal neurons through the suppression of ROS accumulation and up-regulation of mature BDNF expression and CREB phosphorylation.

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Section: Discussionmentioning

confidence: 83%

“…Hippocampal cell death is a major factor in the progression of neurological disorders, including cognitive impairments and cerebrovascular diseases, such as stroke, and is associated with development of cognitive decline and dementia (Lee et al, 2012;Debette, 2013).…”

Section: Introductionmentioning

confidence: 99%

Beneficial Effects ofPolygonum multiflorumon Hippocampal Neuronal Cells and Mouse Focal Cerebral Ischemia

Ahn

Kim

et al. 2015

Am. J. Chin. Med.

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“…22) Neuronal death in the hippocampus is a key determinant of the progression of memory impairment in Alzheimer's disease. 23) We examined whether C. ternata can block neuronal cell death using the HT22 hippocampal cell line. To examine the viability of HT22 cells in the presence of the C. ternata extract, cells were treated with various concentrations of the extract (0, 12.5, 25, 50, or 100 µg/mL) for 24 h. The C. ternata extract exhibited no cytotoxicity up to 100 µg/mL in HT22 cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

Neuroprotective Effect of <i>Corydalis ternata</i> Extract and Its Phytochemical Quantitative Analysis

et al. 2017

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The tubers of Corydalis ternata have been used to treat cardiovascular diseases such as hypertension and cardiac arrhythmia. Its active components have anticholinesterase, antiamnesic, and anti-inflammatory activities, and analgesic effects. In the present study, we performed quantitative analyses of the two components of C. ternata, coptisine and berberine, using HPLC. A 70% ethanol extract of C. ternata was prepared and the two components were separated using a C-18 analytical column on a gradient solvent system of acetonitrile and 0.1% (v/v) aqueous trifluoroacetic acid. Recordings were performed at a UV wavelength of 265 nm for two standard components. The established analytical method showed high linearity (correlation coefficient (r) 1.0000) and proper precision (0.49-3.88%), accuracy (97.88-102.7%), and recovery (95.12-103.79%) for two standard components. The amount of the coptisine and berberine was 4.968 0.089 mg/g and 3.73 0.075 mg/g, respectively. In addition, we investigated the effects of coptisine and berberine on acetylcholinesterase activity and amyloid-β aggregation, which are major biomarkers of dementia. Coptisine and berberine decreased acetylcholinesterase activity in a dose-dependent manner (IC 50 0.74 and 0.48 µM, respectively). The C. ternata extract exerted an antioxidant activity by stimulating the radical scavenging activity of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), but not 2,2-diphenyl-1-picrylhydrazyl (DPPH). Furthermore, the C. ternata extract reversed the hydrogen peroxide-induced death of HT22 hippocampal cells, indicating its neuroprotective effect. Our results suggest the potential of C. ternata as a therapeutic agent against dementia via the inhibition of acetylcholinesterase activity and neuronal cell death.

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“…Damage of hippocampal neurons can explain the progress of learning and memory impairment in a wide number of brain disorders [6,16]. Effective new therapeutic strategies are needed to prevent hippocampal neuronal deaths, which can ameliorate cognitive dysfunction of many neurological disorders.…”

Section: Introductionmentioning

confidence: 99%

New Four-herb Formula Ameliorates Memory Impairments via Neuroprotective Effects on Hippocampal Cells

Ahn¹,

Choi²,

Shin³

et al. 2016

Journal of Life Science

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The current study was conducted to evaluate beneficial effects of a new formula (CWC-9) using four traditional Oriental medicinal herbs, Cynanchum wilfordii, Rehmannia glutinosa, Polygala tenuifolia, and Acorus gramineus, on hippocampal cells and memory function. To examine the neuroprotective effects of a new four-herb extract, cell viability, cytotoxicity, and reactive oxygen species (ROS) assays were performed in HT22 cells and behavioral tests (Morris water maze and passive avoidance retention), Western blot, and immunohistochemistry were performed in a mouse model of focal cerebral ischemia. In HT22 hippocampal cells, pretreatment with CWC-9 resulted in significantly reduced glutamate-induced cell death with suppression of ROS accumulation caused by glutamate. In a mouse model of focal cerebral ischemia, we observed significant improvement of spatial and short-term memory function by treatment with CWC-9. Phosphorylated p38 mitogen-activated protein kinases (MAPK) in hippocampus of ischemic mice were decreased by treatment with CWC-9, but phosphorylated phosphatidylinositol-3 kinase (PI3K) and cAMP response element binding protein (CREB) were significantly enhanced. By immunohistochemical analysis, we confirmed higher expression of phosphorylation of CREB in the hippocampal neurons of CWC-9 treated mice. These results suggest that new multi-herb formula CWC-9 mainly exerted beneficial effects on cognitive function through regulation of neuroprotective signaling pathways associated with CREB.

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Cognition-enhancing and neuroprotective activities of the standardized extract of Betula platyphylla bark and its major diarylheptanoids

Cited by 33 publications

References 21 publications

Beneficial Effects ofPolygonum multiflorumon Hippocampal Neuronal Cells and Mouse Focal Cerebral Ischemia

Beneficial Effects ofPolygonum multiflorumon Hippocampal Neuronal Cells and Mouse Focal Cerebral Ischemia

Neuroprotective Effect of <i>Corydalis ternata</i> Extract and Its Phytochemical Quantitative Analysis

New Four-herb Formula Ameliorates Memory Impairments via Neuroprotective Effects on Hippocampal Cells

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