Neuroprotective Effect of <i>Corydalis ternata</i> Extract and Its Phytochemical Quantitative Analysis

Abstract

The tubers of Corydalis ternata have been used to treat cardiovascular diseases such as hypertension and cardiac arrhythmia. Its active components have anticholinesterase, antiamnesic, and anti-inflammatory activities, and analgesic effects. In the present study, we performed quantitative analyses of the two components of C. ternata, coptisine and berberine, using HPLC. A 70% ethanol extract of C. ternata was prepared and the two components were separated using a C-18 analytical column on a gradient solvent system of acetonitrile and 0.1% (v/v) aqueous trifluoroacetic acid. Recordings were performed at a UV wavelength of 265 nm for two standard components. The established analytical method showed high linearity (correlation coefficient (r) 1.0000) and proper precision (0.49-3.88%), accuracy (97.88-102.7%), and recovery (95.12-103.79%) for two standard components. The amount of the coptisine and berberine was 4.968 0.089 mg/g and 3.73 0.075 mg/g, respectively. In addition, we investigated the effects of coptisine and berberine on acetylcholinesterase activity and amyloid-β aggregation, which are major biomarkers of dementia. Coptisine and berberine decreased acetylcholinesterase activity in a dose-dependent manner (IC 50 0.74 and 0.48 µM, respectively). The C. ternata extract exerted an antioxidant activity by stimulating the radical scavenging activity of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), but not 2,2-diphenyl-1-picrylhydrazyl (DPPH). Furthermore, the C. ternata extract reversed the hydrogen peroxide-induced death of HT22 hippocampal cells, indicating its neuroprotective effect. Our results suggest the potential of C. ternata as a therapeutic agent against dementia via the inhibition of acetylcholinesterase activity and neuronal cell death.

A review on analytical methods for natural berberine alkaloids

“…In these methods, UV detection was sensitive enough for the analysis of BAs. Besides UV detectors, DAD detectors have also been used in many analytical methods, especially in the analysis of multi-component samples [20][21][22][23][24]. Mahoniae Caulis was selected as an example to develop and validate the method for the simultaneous determination of four alkaloids (berberine, columbamine, jatrorrhizine, and palmatine) using HPLC-DAD at 345 nm with an Agilent Zorbax SB-AQ C18 column [20].…”

In Silico Analysis of the Association Relationship between Neuroprotection and Flavors of Traditional Chinese Medicine Based on the mGluRs

“…More specifically, the TCMs corresponding to mGluR І and mGluR ІІІ mostly possess bitter flavor and pungent flavor; the mGluR ІІ is relevant to bitter flavor. Many TCMs have been proven to be effective Chinese medicines to treat neurological diseases, including Corrydalis yanhusuo (Yan Hu Suo) [ 36 ], Psoralea corylifolia Linn (Bu Gu Zhi) [ 37 ], and Radix Paeoniae Alba (Bai Shao) [ 38 ]. Specifically, Corrydalis yanhusuo [ 36 ] and Psoralea corylifolia Linn [ 37 ] play neuroprotective effects through treatment of dementia and oxidative stress, respectively.…”

“…Many TCMs have been proven to be effective Chinese medicines to treat neurological diseases, including Corrydalis yanhusuo (Yan Hu Suo) [ 36 ], Psoralea corylifolia Linn (Bu Gu Zhi) [ 37 ], and Radix Paeoniae Alba (Bai Shao) [ 38 ]. Specifically, Corrydalis yanhusuo [ 36 ] and Psoralea corylifolia Linn [ 37 ] play neuroprotective effects through treatment of dementia and oxidative stress, respectively. In addition, Radix Paeoniae Alba [ 38 ] could treat Parkinson’s disease by reducing the MPTP-induced toxicity.…”

Identification and Selection of Reference Genes for Quantitative Transcript Analysis in Corydalis yanhusuo

“…Validating reference genes under certain environmental treatments and in different species is necessary [44,45]. Though the extract of C. yanhusuo has been used in promoting blood circulation, inhibiting cancer cell proliferation, and as an analgesic agent, no reliable reference genes have been selected in this species [2,16,46]. In order to determine the most reliable reference gene, 12 candidate genes were chosen based on the TAIR dataset and previous research.…”

“…Corydalis yanhusuo is a plant from the Papaveraceae family and is widely used to treat drug addiction and as analgesics in China [1]. Abundant pharmacological effects on humans have been identified in the extracts of C. yanhusuo (tuber), such as pain relief, anti-tumor effects, and promotion of blood circulation [2,3]. Benzylisoquinoline alkaloids are the main biological components produced by C. yanhusuo, and previous studies have found that dehydrocorybulbine, which is found in the extract of C. yanhusuo, is a kind of alkaloid that can exhibit antagonistic activity by acting on dopamine receptors [4,5].…”