Coherent Fluctuation Nephelometry: A Rapid Method for Urine Screening for Bacterial Contamination

Гурьев, А. С.; Volkov, A. Yu.; Долгушин, И. И.; Поспелова, А. В.; Rastopov, S. F.; Савочкина, А. Ю.; Сергиенко, В. И.

doi:10.1007/s10517-015-2902-0

Cited by 5 publications

(3 citation statements)

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“…При исследовании начальной концентрации микроорганизмов в 208 образцах мочи взрослых на КФН-анализаторе [2] и при параллельном посеве на плотные питательные среды выявили 31,7% положительных проб. В сопоставлении результатов, диагностическая информативность скрининга на приборе составила: чувствительность -91%, специфичность -46%.…”

Section: опыт использования когерентной флуктуационной нефелометрии дunclassified

“…Для оценки такой возможности было проведено второе исследование, в котором мы определяли чувствительность культур бактерий в образцах мочи пациентов с бактериурией после их подращивания на КФН-анализторе (в течение 1 ч) без выделения изолятов [7]. Было исследовано 182 пробы мочи пациентов, находящихся в урологической клинике, на наличие инфекции на КФН-анализаторе (аналогично работам [2,3]), для валидации метода использовали посев на плотные среды. Для анализа был отобран 21 положительный образец, в котором регистрировался микробный рост в течение 1 ч на приборе.…”

Section: опыт использования когерентной флуктуационной нефелометрии дunclassified

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Coherent fluctuation nephelometry in clinical microbiology

Гурьев

Шалатова

Русанова

et al. 2019

Russian Journal of Infection and Immunity

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In this article data concerning coherent fluctuation nephelometry (CFN) use in clinical microbiology is presented. CFN-analyzer allows to solve two important problems – fast urine screening for bacteriuria within 2-4 hours and antibiotic susceptibility testing within 3-6 hours. Altogether more than 650 urine samples were tested, and the effectivity of CFN-analyzer for preliminary selection of samples for further analysis was shown. Method allows to detect negative samples, reducing the number of urine analyses by 70-80%. Simultaneous analysis of growth curves and concentration of microorganisms shows high sensitivity and specificity (95.2% и 96.9%). Also more than 250 antibiotic susceptibility tests were performed using CFN-analyzer to show its effectiveness for determination of resistant properties of both pure cultures and urine microflora without isolation of bacteria. The agreement with traditional methods was from 84% to 88%. The use of CFN-analyzer with express methods of identification of microorganisms (chromogenic nutrient broths or mass-spectrometry) allows to make full urine analysis within 1-2 days. In the future CFN-analyzer gives an opportunity to screen different human biological liquids, and finds an application for other microbiological tasks, including standardization and speeding-up in sanitary bacteriology.

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Section: опыт использования когерентной флуктуационной нефелометрии дunclassified

Coherent fluctuation nephelometry in clinical microbiology

Гурьев

Шалатова

Русанова

et al. 2019

Russian Journal of Infection and Immunity

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“…High sensitivity of CFN allows not only to record growth curves of microorganisms at low concentrations, but also to estimate the concentration of microorganisms in urine samples by its turbidity. CFN-analyzers were used for bacteriuria screening by growth curve and by concentration of microorganisms separately [12] and also for antibiotic susceptibility testing [13] .…”

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Coherent fluctuation nephelometry as a promising method for diagnosis of bacteriuria

Гурьев

Yudina

Lazareva

et al. 2018

Practical Laboratory Medicine

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ObjectivesSpecialized analyzers are used to automate the diagnosis of bacteriuria in laboratory practice. They are based on analysis of microorganisms concentration in urine samples or recording the growth of urine microflora. Coherent fluctuation nephelometry (CFN) has high sensitivity and allows analyzing both parameters simultaneously. The aim of the study is to compare the effectiveness of CFN-based and flow cytometry based analyzers.Design and methodsTotal 117 urine samples from children were studied in parallel using the CFN-analyzer and UF-1000i (Sysmex), the results were confirmed by conventional microbiological methods.ResultsIn 21 urine samples (18%), significant bacteriuria was determined (≥104 CFU/ml). The best diagnostic indicators were obtained while testing urine samples using the CFN-analyzer. The most efficient bacteriuria diagnosis is achieved by simultaneous analyses of microorganisms concentration in urine and growth of urine microflora (sensitivity – 95.2%, specificity – 96.9%, positive predictive value – 87%, negative predictive value – 98.9%, diagnostic odds ratio – 81.7, positive likelihood ratio – 30.5, negative likelihood ratio– 0.049, area under curve in ROC-analysis – 0.987). The CFN-analyzer allows the preliminary selection of negative urine samples, which do not require further analysis by conventional microbiological methods, thereby decreasing the number of cultures by 80.3%.ConclusionsThis study suggests that the CFN-analyzer is the effective tool for bacteriuria screening in children.

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