Coherent phase microscopy of intracellular processes

Tychinskii, V. P.

doi:10.3367/ufnr.0171.200106e.0649

Cited by 23 publications

(17 citation statements)

References 20 publications

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“…Laser interference microscopy is based on the measurement of the local phase shifts of the laser beam transmitted through an object [27][28][29]. Figure 1 illustrates the principle of the interference microscope.…”

Section: Experimental Techniquementioning

confidence: 99%

Unraveling Cell Processes: Interference Imaging Interwoven with Data Analysis

Brazhe

Pavlov

et al. 2006

J Biol Phys

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The paper presents results on the application of interference microscopy and wavelet-analysis for cell visualization and studies of cell dynamics. We demonstrate that interference imaging of erythrocytes can reveal reorganization of the cytoskeleton and inhomogenity in the distribution of hemoglobin, and that interference imaging of neurons can show intracellular compartmentalization and submembrane structures. We investigate temporal and spatial variations of the refractive index for different cell types: isolated neurons, mast cells and erythrocytes. We show that the refractive dynamical properties differ from cell type to cell type and depend on the cellular compartment. Our results suggest that low frequency variations (0.1-0.6 Hz) result from plasma membrane processes and that higher frequency variations (20)(21)(22)(23)(24)(25)(26) are related to the movement of vesicles. Using double-wavelet analysis, we study the modulation of the 1 Hz rhythm in neurons and reveal its changes under depolarization and hyperpolarization of the plasma membrane. We conclude that interference microscopy combined with wavelet analysis is a useful technique for non-invasive cell studies, cell visualization, and investigation of plasma membrane properties.

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Section: Experimental Techniquementioning

confidence: 99%

Unraveling Cell Processes: Interference Imaging Interwoven with Data Analysis

Brazhe

Pavlov

et al. 2006

J Biol Phys

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“…These changes are similar to the nucleolar response to direct inhibitors of transcription. Hence, quantitative parameters of coherent phase microscopy describe common reaction of the nucleolus to stress; reduction of optical thickness of the nucleolus is a component of this reaction.The method of coherent phase microscopy (CPM) is based on measurement of the refraction value, depending on the morphology and function of the object [2,4,10]. We previously demonstrated the possibility of quantitative analysis of the structure and metabolism of an extensive class of microobjects, from chloroplasts and spores to isolated organelles and eukaryotic cells [1,3,[8][9][10].…”

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confidence: 99%

Reduction of phase thickness is a characteristic reaction of the nucleoli to toxicity as shown by coherent phase microscopy

et al. 2007

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Optical parameters of human cell nucleoli (HCT116 colorectal cancer cells) in depolymerization of microtubules and depletion of intracellular ATP pool were studied by coherent phase microscopy. These influences were associated with a rapid (recorded within the first minutes) reduction of the phase thickness of the nucleoli. These changes are similar to the nucleolar response to direct inhibitors of transcription. Hence, quantitative parameters of coherent phase microscopy describe common reaction of the nucleolus to stress; reduction of optical thickness of the nucleolus is a component of this reaction.

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“…Laser interference microscopy is also used for the studies of dynamic processes in nerve cells. Regular local changes in optical density and/or geometry of the neuron and axon correlate with its rhythmic activity or effects of neurotransmitters [1,4].…”

mentioning

confidence: 99%