Coherent spectroscopic methods for monitoring pathogens, genetically modified products and nanostructured materials in colloidal solution

Abstract

Abstract. We developed the new automatic method that combines the method of forced luminescence and stimulated Brillouin scattering. This method is used for monitoring pathogens, genetically modified products and nanostructured materials in colloidal solution. We carried out the statistical spectral analysis of pathogens, genetically modified soy and nano-particles of silver in water from different regions in order to determine the statistical errors of the method. We studied spectral characteristics of these objects in water to perform the initial identification with 95% probability. These results were used for creation of the model of the device for monitor of pathogenic organisms and working model of the device to determine the genetically modified soy in meat.We developed the coherent spectroscopic method for the monitoring of the pathogenic organisms directly in water pipe-line, genetically modified products and nanostructured materials in colloidal solution. The method is based on an analysis of spectral characteristics of stimulated radiation, passed through the solution.We studied transmission IR spectra of a number of pathogens (salmonella, viruses of herpes genitaler, hepatitis A and C, grippe A and B ecd) and nanosilver solutions and luminescence of nanomarkers. In our experiments, the laser radiation passed through a quartz cell with water solutions of the pathogens, nanosilver or nanomarkers. Spectra were analyzed by the spectrum analyzer "Agilent" (USA) with spectral resolution 0.5 nm. The solid DPSS lasers ("Laser-export Co. Ltd.", Russia) with wavelengths 1017, 810, 670, 532, 480 nm were used as the sources of exciting radiation. The input/output waveguides bring the exciting and passing radiations from laser to the cell and from cell to the analyzer, respectively.We found that exciting radiations with wavelengths 1017 and 810 nm induce the stimulated Brillouin scattering in spectra of the water containing pathogen DNAs. We revealed that peak positions and widths of "fingerprints" for pathogens under study, and optical densities of these bands were proportional pathogen content, if their content was