No abstract

No abstract

Presented is a new method for the recognition of live or dead pathogens in water. This method is based on the diagnostics of non-linear effects that comprise two phenomena: an induced luminescence of DNA under the influence of laser radiation, and a stimulated Brillouin scattering (SBS). The following model parameters were selected for the identification: the peak positions of spectral lines corresponding to pathogens and the difference between two wavelengths corresponding, respectively, to a laser mode maximum, and to a peak of the spectral line of a pathogen. A large volume of experimental data has been obtained and statistical analysis using Student's test was carried out in order to determine the probability of the detection of spectra. The dynamics of the spectra of direct dispersion have been studied for a number of bacteria. Based on these results, the method and the device were developed. That device was tested in a water pipe line. In addition, we have studied the dynamics of changes in the spectra for solutions containing live bacteria and dead cells.

No abstract

Hygienic standards to the quality of potable water require continuous monitoring of the absence of pathogenic microorganisms directly in water flow. Despite a great number of laboratory devices for checking the quality of potable water, there are no express analyzers for monitoring of pathogenic organisms, which could be embedded directly into the automatic checkout systems. The reasons of it are low concentration of pollutions and the presence of additional effects, which impede automatic data processing. The new method, the express analysis of pathogens in water, was developed. It shall be mentioned that the proposed method of express diagnostics allows detection of infectious agents in the water in minutes based on nonlinear effects. This research has, hopefully, laid the foundation for development of a prototype for determination of the content of the genetically modified soy in meat products. The inventive methods can be recommended for DNA diagnostics in medicine ,veterinary sciences and insanitation. The main advantage of this method is that there is no need for DNA isolation. It is sufficient to create a suspension of the product by centrifugation.