Monitoring toxicants by stimulated Mandelshtam-Brillouin scattering (SMBS) in a turbulent water flow

Moguilnaya, T Yu; Bobkov, P V; Tomilin, V. A.

doi:10.1088/1742-6596/414/1/012023

Cited by 2 publications

(3 citation statements)

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“…The similar density of radiation is reached due to combination of the luminescence fields and laser radiation [2] giving the characteristic line. The modal frequency of this line depends on type DNA and accordingly from type of a pathogenic organism [3].…”

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confidence: 76%

Coherent spectroscopic methods for monitoring pathogens, genetically modified products and nanostructured materials in colloidal solution

et al. 2016

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Abstract. We developed the new automatic method that combines the method of forced luminescence and stimulated Brillouin scattering. This method is used for monitoring pathogens, genetically modified products and nanostructured materials in colloidal solution. We carried out the statistical spectral analysis of pathogens, genetically modified soy and nano-particles of silver in water from different regions in order to determine the statistical errors of the method. We studied spectral characteristics of these objects in water to perform the initial identification with 95% probability. These results were used for creation of the model of the device for monitor of pathogenic organisms and working model of the device to determine the genetically modified soy in meat.We developed the coherent spectroscopic method for the monitoring of the pathogenic organisms directly in water pipe-line, genetically modified products and nanostructured materials in colloidal solution. The method is based on an analysis of spectral characteristics of stimulated radiation, passed through the solution.We studied transmission IR spectra of a number of pathogens (salmonella, viruses of herpes genitaler, hepatitis A and C, grippe A and B ecd) and nanosilver solutions and luminescence of nanomarkers. In our experiments, the laser radiation passed through a quartz cell with water solutions of the pathogens, nanosilver or nanomarkers. Spectra were analyzed by the spectrum analyzer "Agilent" (USA) with spectral resolution 0.5 nm. The solid DPSS lasers ("Laser-export Co. Ltd.", Russia) with wavelengths 1017, 810, 670, 532, 480 nm were used as the sources of exciting radiation. The input/output waveguides bring the exciting and passing radiations from laser to the cell and from cell to the analyzer, respectively.We found that exciting radiations with wavelengths 1017 and 810 nm induce the stimulated Brillouin scattering in spectra of the water containing pathogen DNAs. We revealed that peak positions and widths of "fingerprints" for pathogens under study, and optical densities of these bands were proportional pathogen content, if their content was

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confidence: 76%

Coherent spectroscopic methods for monitoring pathogens, genetically modified products and nanostructured materials in colloidal solution

et al. 2016

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“…To identify the main informative parameters of the method, the experimental samples were statistically processed. Initial comparative analysis was based on Student's test [6].…”

Section: Spectra Of Bacteriamentioning

confidence: 99%

“…We developed proprietary software for the diagnostics of the pathogens [6]. The informative parameters for diagnostics were: (a) the reference spectra previously registered -concentration live 10 3 cml −1 ; -concentration live 10 5 c ml −1 ; -concentration live 10 8 c ml −1 ; -concentration dead 10 5 c ml −1 .…”

Section: Spectra Of Bacteriamentioning

confidence: 99%

Detecting presence of live and dead pathogenic organisms in water and the water solutions by laser spectroscopy

Moguilnaya¹,

Суминов²,

Ignatov

2017

Laser Phys. Lett.

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Presented is a new method for the recognition of live or dead pathogens in water. This method is based on the diagnostics of non-linear effects that comprise two phenomena: an induced luminescence of DNA under the influence of laser radiation, and a stimulated Brillouin scattering (SBS). The following model parameters were selected for the identification: the peak positions of spectral lines corresponding to pathogens and the difference between two wavelengths corresponding, respectively, to a laser mode maximum, and to a peak of the spectral line of a pathogen. A large volume of experimental data has been obtained and statistical analysis using Student's test was carried out in order to determine the probability of the detection of spectra. The dynamics of the spectra of direct dispersion have been studied for a number of bacteria. Based on these results, the method and the device were developed. That device was tested in a water pipe line. In addition, we have studied the dynamics of changes in the spectra for solutions containing live bacteria and dead cells.

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Monitoring toxicants by stimulated Mandelshtam-Brillouin scattering (SMBS) in a turbulent water flow

Cited by 2 publications

References 1 publication

Coherent spectroscopic methods for monitoring pathogens, genetically modified products and nanostructured materials in colloidal solution

Coherent spectroscopic methods for monitoring pathogens, genetically modified products and nanostructured materials in colloidal solution

Detecting presence of live and dead pathogenic organisms in water and the water solutions by laser spectroscopy

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