COI1-Dependent Expression of an Arabidopsis Vegetative Storage Protein in Flowers and Siliques and in Response to Coronatine or Methyl Jasmonate

Benedetti, Celso Eduardo; Xie, Daoxin; Turner, John G.

doi:10.1104/pp.109.2.567

Cited by 121 publications

(91 citation statements)

References 34 publications

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“…As shown in Figure 6, the VSP1 transcript was markedly increased under darkness, and it was further increased in response to the MeJA treatment. This result is consistent with a previous report (Benedetti et al 1995), confirming that our JA treatment experiment was rigorously performed. On the other hand, the transcript level of EPI1 was increased by not only MeJA but also the mock treatment.…”

Section: Expression Of Epi1 Is Upregulated By Darkness and Ja Treatmentssupporting

confidence: 83%

The ERF transcription factor EPI1 is a negative regulator of dark-induced and jasmonate-stimulated senescence in <i>Arabidopsis</i>

Chung

Nakano

Fujiwara

et al. 2016

Plant Biotechnology

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Identification of the factors involved in the regulation of senescence and the analysis of their function are important for both a biological understanding of the senescence mechanism and the improvement of agricultural productivity. In this study, we identified an ERF gene termed "ERF gene conferring Postharvest longevity Improvement 1" (EPI1) as a possible regulator of senescence in Arabidopsis. We found that EPI1 possesses transcriptional repression activity and that the transgenic plants overexpressing EPI1 and expressing its chimeric repressor, EPI1-SRDX, commonly suppressed the darkness-induced senescence in their excised aerial parts. These transgenic plants additionally maintained a high level of chlorophyll, even after the methyl jasmonate (MeJA) treatment, which stimulated senescence in the dark. In addition, we found that senescence-induced and -reduced genes are down-and upregulated, respectively, in the MeJA-treated transgenic plants under darkness. Our results suggest that EPI1 functions as a negative regulator of the dark-induced and JA-stimulated senescence.

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Section: Expression Of Epi1 Is Upregulated By Darkness and Ja Treatmentssupporting

confidence: 83%

The ERF transcription factor EPI1 is a negative regulator of dark-induced and jasmonate-stimulated senescence in <i>Arabidopsis</i>

Chung

Nakano

Fujiwara

et al. 2016

Plant Biotechnology

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“…When assayed for JA-inducible expression of Arabidopsis VEGETATIVE STORAGE PROTEIN (AtVSP) (Benedetti et al, 1995) and LIPOXYGENASE2 (LOX2) (Bell et al, 1995), the cos1 mutation is able to restore defect of the JA-inducible gene expression in coi1-2. Figure 1C shows that the levels of AtVSP and LOX2 transcripts upon JA induction are higher in cos1 coi1-2 than those in coi1-2.…”

Section: Mutant By Cos1mentioning

confidence: 99%

“…Specific primers were designed based on their DNA sequences to PCR amplify AtVSP (Benedetti et al, 1995), LOX2 (Bell et al, 1995), and SEN4 (Nam, 1997) and used as RNA gel blot probes. The probe labeling and RNA gel blot hybridization methods were described previously (Xu et al, 2001).…”

Section: Rna Gel Blot and Rt-pcr Analysismentioning

confidence: 99%

COS1: An Arabidopsis coronatine insensitive1 Suppressor Essential for Regulation of Jasmonate-Mediated Plant Defense and Senescence

et al. 2004

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The Arabidopsis thaliana CORONATINE INSENSITIVE1 (COI1) gene encodes an F-box protein to assemble SCF COI1 complexes essential for response to jasmonates (JAs), which are a family of plant signaling molecules required for many essential functions, including plant defense and reproduction. To better understand the molecular basis of JA action, we screened for suppressors of coi1 and isolated a coi1 suppressor1 (cos1) mutant. The cos1 mutation restores the coi1-related phenotypes, including defects in JA sensitivity, senescence, and plant defense responses. The COS1 gene was cloned through a map-based approach and found to encode lumazine synthase, a key component in the riboflavin pathway that is essential for diverse yet critical cellular processes. We demonstrated a novel function for the riboflavin pathway that acts downstream of COI1 in the JA signaling pathway and is required for suppression of the COI1-mediated root growth, senescence, and plant defense.

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“…A high enrichment for genes encoding protein kinases with putative functions in signal perception or signal transduction was observed among the genes upregulated in wrky11.1 at 5 h after inoculation (8 of 44 genes; see Supplemental Table 3A online) and in wrky17.1 at 2 h after inoculation (18 of 110 genes; see Supplemental Table 3C online). In addition, two JA-associated genes, LOX2 and AOS, were analyzed, because a high proportion of genes downregulated in wrky11.1 at 5 h (see Supplemental Table 3B online) have been described in the literature as being JA-responsive, including VSP1, VSP2, and COR1 (Benedetti et al, 1995(Benedetti et al, , 1998Tsuchiya et al, 1999), or are strongly JA-inducible according to transcriptome databases (see Supplemental Figure 5 online). Three of the genes, LOX2, AOS, and JMT, encode enzymes of JA biosynthesis or derivatization (Bell et al, 1995;Laudert et al, 1996;Seo et al, 2001).…”

Section: Functional Overlap Between Wrky11 and Wrky17 In The Regulatimentioning

confidence: 99%

The Transcription Factors WRKY11 and WRKY17 Act as Negative Regulators of Basal Resistance in Arabidopsis thaliana

et al. 2006

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Transcription factors are believed to play a pivotal role in the activation and fine-tuning of plant defense responses, but little is known about the exact function of individual transcription factors in this process. We analyzed the role of the IId subfamily of WRKY transcription factors in the regulation of basal resistance to Pseudomonas syringae pv tomato (Pst). The expression of four members of the subfamily was induced upon challenge with virulent and avirulent strains of Pst. Mutant analyses revealed that loss of WRKY11 function increased resistance toward avirulent and virulent Pst strains and that resistance was further enhanced in wrky11 wrky17 double mutant plants. Thus, WRKY11 and WRKY17 act as negative regulators of basal resistance to Pst. Genome-wide expression analysis and expression studies of selected genes in single and double mutants demonstrated that both transcription factors modulate transcriptional changes in response to pathogen challenge. Depending on the target gene, WRKY11 and WRKY17 act either specifically or in a partially redundant manner. We demonstrate complex cross-regulation within the IId WRKY subfamily and provide evidence that both WRKY transcription factors are involved in the regulation of Pst-induced jasmonic acid-dependent responses. These results provide genetic evidence for the importance of WRKY11 and WRKY17 in plant defense.

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COI1-Dependent Expression of an Arabidopsis Vegetative Storage Protein in Flowers and Siliques and in Response to Coronatine or Methyl Jasmonate

Cited by 121 publications

References 34 publications

The ERF transcription factor EPI1 is a negative regulator of dark-induced and jasmonate-stimulated senescence in <i>Arabidopsis</i>

The ERF transcription factor EPI1 is a negative regulator of dark-induced and jasmonate-stimulated senescence in <i>Arabidopsis</i>

COS1: An Arabidopsis coronatine insensitive1 Suppressor Essential for Regulation of Jasmonate-Mediated Plant Defense and Senescence

The Transcription Factors WRKY11 and WRKY17 Act as Negative Regulators of Basal Resistance in Arabidopsis thaliana

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